International
Pharma Conference and Expo

May 2-4, 2018 | Rome, Italy.

Program Schedule

  • Keynote Speaker

    Time:
    09:30-10:00

    Title

    Title: Neuroinflammation and Microglial Constitutive COX-1 Inhibition

    Maria Grazia Perrone
    University of Bari, Italy.
    Biography
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    Biography

    Maria Grazia Perrone
    University of Bari, Italy.

    Researcher of the Department of Pharmacy – Pharmaceutical Sciences of Bari University, Italy. After a stage at the University of Bioverfahrenstechnik (Stuttgart-Germany), her scientific interests have been devoting to clarify the Cyclooxygenases role in inflammation as the earliest step of both neurodegenerative disorders and oncology. Author of approximately 50 scientific publications on international journals and Principal Investigator of scientific projects, among which the grant supporting my current studies [First AIRC Grant-MFAG2015 (Project Id. 17566)].



    Abstract
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    Abstract

    Maria Grazia Perrone
    University of Bari, Italy.

    Neuroinflammation, as the earliest stage of several neurological and neurodegenerative diseases, takes please about 15-20 years before the appearance of specific neurodegenerative clinical symptoms. Among the known mechanisms involved into the neuroinflammatory complex network, the cyclooxygenase-1 (COX-1) (predominantly localized in microglia) plays a previously unrecognized role in the neuroinflammation as demonstrated by the attenuation of the inflammatory response and neuronal loss due to the genetic ablation or pharmacological inhibition of COX-1 activity. The lack of drugs to treat diseases involving the central nervous system (CNS) also resides into the shield exerted by the blood brain barrier (BBB) matrix. BBB has a low permeability, and the development of drugs able to penetrate through its network is one of the challenges of all scientists involved in projecting medicines having active principle ingredients targeting the CNS diseases. A commonly used strategy to overcome this drawback consists to incorporate into the pharmacological active molecule a sugar moiety (i.e. glucose or galactose), in turn capable to “carry” the entire molecule into the CNS by the GLUT-1 carrier, which is located on the membrane of the endothelial cells. In this context, a set of novel compounds endowed with inhibitory activity with cyclooxygenase-1 and GLUT-1 substrate will be presented. Specifically, their design rationale and biological activity will particularly detailed. The work here presented is financially supported by First AIRC Grant-MFAG2015 (Project Id. 17566) “COX inhibitors in conjunction with chemotherapy to target multiple myeloma active disease”.

    Keynote Speaker

    Time:
    10:00-10:30

    Coffee Break 10:30-10:45

    Title

    Title: COXs Inhibition role in Multiple Myeloma

    Maria Laura Pati
    University of Bari, Italy.
    Biography
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    Biography

    Maria Laura Pati
    University of Bari, Italy.

    Maria Laura Pati graduated cum laude in Medicinal Chemistry at the University of Bari (Italy). PhD at the University of Bari (Italy). One year as Visiting Researcher at Washington University in St. Louis (USA) working on the in vitro and in vivo efficacy of novel molecules for resistant tumors treatment. One month as Visiting Researcher at University of Vienna where she gained expertise on confocal microscopy analysis. Currently, she is granted by First AIRC Grant-MFAG2015 (Project Id. 17566) for the project “COX inhibitors in conjunction with chemotherapy to target multiple myeloma active disease”.



    Abstract
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    Abstract

    Maria Laura Pati
    University of Bari, Italy.

    Multiple Myeloma (MM) is an incurable malignant disease of plasma cells. PGE2 and other prostaglandins (PGs), synthesized by cyclooxygenase (COX)-mediated arachidonic acid transformation, are crucial mediators of inflammation and angiogenesis, and support the growth of several tumors. Two COX isoforms have been identified, COX-1 and COX-2. Despite considerable data concerning COX expression in solid tumors are available, their role in hematologic malignancies and in MM has been little investigated. Non-steroidal anti-inflammatory drugs (NSAIDs), mainly acting as COX inhibitors, have shown to be immunotherapeutic agents in several malignancies, including hematological tumors and MM. Some studies proven the usefulness, in the treatment of MM, of COX inhibitors like indomethacin, ibuprofen, NS-398, celecoxib endowed with a different grade of selectivity towards the inhibition of the two COX isoforms. The pharmaceutic effect of SC-560, Mofezolac, as selective COX-1 inhibitors, Celecoxib, as a selective COX-2 inhibitor, and Aspirin, Ibuprofen with a different grade of selectivity towards COX isoforms, have been evaluated by us on cellular COX status (protein expression, and enzymatic activity) of widely used hMM cell lines (i.e., U937, RPMI-8226, HPC, ARH77). COX-1 and COX-2 role in MM active disease, as well as the usefulness of their selective or non-selective inhibitors to be used as therapeutic agents to strength the action of the clinically used anticancer drugs (i.e., dexamethasone, bortezomib and thalidomide) will be presented. The work here presented is financially supported by First AIRC Grant-MFAG2015 (Project Id. 17566).

    Sessions:
    Pharmacology ,Clinical Pharmacology & Molecular Pharmacology & Pharmaceutical Technology & Pharmacy Practice

    Time:
    10:45-11:10

    Title: Orally Bioavailable Antimalarial 4(1H)-Quinolone and 4(1H)-Quinolone Prodrugs with Single-Dose Cures

    Roman Manetsch
    Northeastern University, USA

    Biography
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    Biography

    Roman Manetsch
    Northeastern University, USA

    Roman Manetsch received his PhD degree in 2002 from the University of Basel (Switzerland) working with Professor Wolf-Dietrich Woggon. After a postdoctoral experience with Professor K. Barry Sharpless at the Scripps Research Institute, he started his independent career as an Assistant Professorat the Department of Chemistry at the University of South Florida (Tampa, Florida). In 2014, Associate Professor Manetsch moved to theDepartment of Chemistry and Chemical Biology and the Department of Pharmaceutical Sciences at Northeastern University (Boston, Massachusetts). His current research focuses on the development offragment-based lead discovery strategies, the development of chemical probes for the study of specific proteins in complex biological matrices, as well as hit-to-lead optimizations of anti-malarial, anti-leishmanial, and anti-bacterial agents.



    Abstract
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    Abstract

    Roman Manetsch
    Northeastern University, USA

    For approximately half a century, 4(1H)-quinolones such as endochin or ICI 56,780 were known to be causal prophylactic and potent erythrocytic stage agents in avian but not in mammalian malaria models. Hit-to-lead optimization of endochin lead to 4(1H)-quinolones ELQ-300 and P4Q-391, which target the liver, the blood as well as the transmitting stages of the parasite (Sci. Transl. Med. 2013, 5, 177ra37). However, poor aqueous solubility severely limits absorption and oral bioavailability and therefore impedes preclinical development of this class of antimalarials Herein, we disclose a general prodrug approach that converts promising lead compounds, such as antimalarial 4(1H)-quinolones, into aminoalkoxycarbonyloxymethyl (amino AOCOM) ethers that display significantly improved aqueous solubility and enhanced oral bioavailability. The prodrug is autarkic, independent of biotransformations, and animal-independent as it activates via a pH-triggered intramolecular cyclization reaction. Amino AOCOM ether prodrugs of antimalarial 4(1H)-quinolones were shown to possess pharmacokinetic and efficacy profiles significantly improved relative to the corresponding parent compounds (> 50-fold improvement of Cmax and AUC). One of the most promising 3-aryl-4(1H)-quinolone preclinical candidates was further shown to provide single-dose cures in a rodent malaria model at an unprecedentedly low oral dose of 3 mg/kg, without the use of an advanced formulation technique.

    Time:
    11:10-11:35

    Title: The Importance of Rapid Antigen Testing on Rational Antibiotic Use

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Biography
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    Biography

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Dr Arslan is head of pharmacology department in the Ankara Yildirim Beyazıt University. His researches are focus on the experimental pathopysiology and inflammation of pulmonary and cardiovascular systems. He is PhD supervisor for pharmacology or toxicology students



    Abstract
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    Abstract

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Rapid antigen testing (RAT) has started to encourage the use of rational antibiotics in primary health care (family medicine department) in February 2017 in Turkey. The RAT is a practical test used to determine the presence of antigens of A group beta hemolytic streptococcus (AGS). This study aims to observe whether there is a difference in the rate of antibiotics prescription between years of 2016 and 2017, in which RAT was not used in the former and used in the later. The most frequent bacterial agents of acute pharyngitis (AP) and tonsillopharyngitis (AT) are AGS. It is quite difficult to distinguish between viral or bacterial agents according to anamnesis, clinical and physical examination findings. Since the result of throat cultures requires 24-48 hours, various methods have been developed for fast determination the factors in AP and AT cases. The RAT test is one of them. The use of RAT is of great importance in reducing the use of irrational antibiotics and the cost of antibiotics. A statistically significant difference was found in the antibiotic prescription of family physicians due to the use of RAT in the selected family medicine departments between the years of 2016 and 2017 (p<0.05). The RAT is a rapid and reliable method for the diagnosis of streptococcal AP and AT in outpatient. When RAT is positive, it indicates streptococcal AP and AT. However, negative detection of the testing does not remove it from the diagnosis. It is also observed that the use of RAT creates an important pharmacoeconomic awareness in physicians. Key Words: Rapid Antigen Testing, Rational antibiotics use, Acute Pharyngitis, Acute tonsillopharyngitis, A group beta hemolytic streptococcus

    Time:
    11:35-12:00

    Title: Sulfasalazine/Dasatinib: A Novel Combined Therapy in Hepatocellular Carcinoma Cell Line

    Marium Shamaa
    Arab Academy for Science, Technology and Maritime Transport, Egypt

    Biography
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    Biography

    Marium Shamaa
    Arab Academy for Science, Technology and Maritime Transport, Egypt

    Marium Shamaa; born 1986, graduate of Elekbal Elkawmeya language school in Alexandria (class 2002), studied pharmaceutical science (2003–2007) at Alexandria University,Egypt. She received her master degree (2012) in Pharmacogenomics (IGSR,Alexandria University) and PhD degree (2016) in Biochemistry and Molecular Biology (IGSR,Alexandria University). She is studying the Clinical Pharmacotherapy Course at the active training company. She is now Lecturer of Biochemistry and Molecular Biology, college of Pharmacy, Arab Academy for Science, Technology and Maritime Science,Alexandria,Egypt and simultaneously a member in the editorial board of the Universal Journal of Pharmaceutical Research. Her professional interests focus Pharmacotherapy, Pharmacogenetics, Oncology and Liver diseases.



    Abstract
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    Abstract

    Marium Shamaa
    Arab Academy for Science, Technology and Maritime Transport, Egypt

    Hepatocellular carcinoma (HCC) is one of the most common human malignancies. Lack of efficient therapy for advanced HCC is a pressing problem worldwide. Dasatinib is an oral dual BCR/ABL and Src family tyrosine kinase inhibitor approved for use in patients with chronic myelogenous leukemia (CML). The main targets of dasatinib, are BCRABL, SRC and GFR. NFκB is one of widely recognized positive regulator of cancer cell proliferation. It could be worthy to combine multikinase inhibitor with NFκB inhibitor for targeting of multiple signaling pathways involved in carcinogenesis. This study aimed to evaluate the potential anti-carcinogenic effects of either dasatinib and/or sulfasalazine on HEPG2 cell line as a model of HCC. The current study was conducted on 4 groups. For determination of the specific doses for the selected drugs MTT assay was done. While for laboratory investigations; cell lysates and nuclear extracts were subjected to ELISA and QRT-PCR. Both drugs have modulated RAF/ERK, BCR/ABL and NFκB pathways. Additionally, sulfasalazine confirmed its antiproliferative effects by lowering cyclin-D1, its apoptotic effect by improving the level of caspase-3, and its antiangiogenic effect by lowering VEGF level in a pattern similar to that of dasatinib. On the molecular level, dasatinib and sulfasalazine downregulated the gene expression level of c-MET. On almost all the parameters, the sulfasalazine effects were more superior when compared to that of dasatinib, while the combination regimen showed the best effects. The author present study showed for the first time the beneficial anticarcinogenic effects of dasatinib and sulfasalazine coadministration on HCC cells.

    Time:
    12:00-12:25

    Title: Glitazones to Gliptins and Gliflozins: Quest For Cardio-Friendly Antidiabetics

    Kiran Dubey
    Jamia Hamdard University, India

    Biography
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    Biography

    Kiran Dubey
    Jamia Hamdard University, India

    Dr. Kiran Dubey (M. Pharm, Ph.D. PGDMM) is currently associated with Jamia Hamdard as Assistant Professor in the Department of Pharmacology, School of Pharmaceutical Education and Research, New Delhi. She has also served in the Medical Information Department of Ranbaxy Laboratories, Systopic Laboratories, Dee Pharma Ltd and Skin Institute and School of Dermatology. Her areas of research include cardiovascular safety profile of NSAIDs, diabetes and related complications. She has guided twenty four post graduate students and three Ph.D. students in the field of Pharmacology and Pharmacy Practice, published articles and has been reviewer for International and National journals of repute.



    Abstract
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    Abstract

    Kiran Dubey
    Jamia Hamdard University, India

    The well-characterized associations between type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) are twofold. First, CVD is the underlying cause of more than 60% deaths in diabetic patients. Second, the hyperglycaemia of T2DM, as measured by the percentage of glycosylated haemoglobin, is believed to increase the risk of coronary artery disease and myocardial infarction (MI). Given this relationship, physicians managing T2DM must remain aware of the cardiovascular effects of their therapeutic choices. Unfortunately, most oral antidiabetic therapies made available to date have not resulted in reduced incidences of MI, stroke or cardiovascular death in diabetic subjects. While metformin appears to be safe, it may lead to lethal lactic acid acidosis, especially in patients of recent MI or heart failure. An increased risk of all-cause mortality and cardiovascular related mortality has been associated with sulfonylureas and their combination with metformin. Meglitinides effects are similar to those of sulfonylureas, due to their analogous mechanism of action. Glitazones are unsafe in NYHA class III or IV due to weight gain and oedema. The cardiovascular safety outcome trials conducted with dipeptidyl peptidase-4 (DPP-4) inhibitors, saxagliptin and alogliptin showed an increase in hospitalization due to heart failure, while sitagliptin demonstrated no such effect. Evidence for cardiovascular benefits of glucagon-like peptide-1 (GLP-1) receptor agonists has been similarly heterogeneous, with liraglutide and semaglutide reducing the risk of composite cardiovascular outcomes, but lixisenatide having no reduction or increase in cardiovascular risk. The trial conducted with sodium-glucose co-transporter-2 (SGLT2) inhibitor, empagliflozin found it to be superior in reducing major cardiac events. However, there was a non-significant increase in silent MI with empagliflozin. While the FDA regulatory mandate to demonstrate the cardiovascular safety in order to approve new antidiabetic drugs, the quest for cardio friendly antidiabetics continues.

    Time:
    12:25-12:50

    Lunch Break: 12:50-14:00

    Title: Androgen Effect on The Muscular Expression of Neurotrophins; New Pharmacological Targets and Possible Modulators

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Biography
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    Biography

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Dr Nasr Alrabadi has completed his bachelor degree in medicine (2009) and received the Jordanian board for practicing medicine (2010). Dr Alrabadi completed his master degree in pharmacology (2012) and has been awarded his PhD in medicine and nanotechnology (2016) from Sydney University. Currently, he is assistant professor at the department of Pharmacology/ faculty of medicine, Jordan University of Science and technology (JUST) and a team leader for the molecular biology and analytical facility at the Nanotechnology institute/ JUST University. As a young researcher, he has 7 publications/ abstracts.



    Abstract
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    Abstract

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Androgens are natural and essential hormones in the development and function of body organs. As well, these hormones can be manufactured and administered to the body aiming to treat different pathological conditions. Anabolic steroids are forms of these manufactured androgens which are excessively used by body builders aiming to gain excessive muscle weight and strength. However, the abuse of anabolic steroids is associated with variety of catastrophic complications. This dose-dependent effect especially with the differences in the prevalence and prognosis of androgen related diseases between different sexes, can indicate to dose and sex-dependent paradoxical effects of these hormones. Our group focus on studying the effect of wide range of androgen (sustanon) dosages/ concentrations on the differential expression of the main and well-known neurotrophins, so called; BDNF, NGF, NT3 and NT4/5. This expression is studied at the level of the heart, skeletal muscles and gastrointestinal wall (muscles) of either male/ female rats or chickens. Exploring this relationship will strengthen our understanding regarding the paradoxical effect of androgen usage among athletes. On the other hand, it may identify possible pharmacological targets and modulators for variety of pathological conditions related to what we call “androgen-neurotrophins axes”.

    Time:
    14:00-14:25

    Title: More than Three Decades of Proton Pump Inhibitors

    Abu Freha Naim
    Ben-Gurion University, Israel

    Biography
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    Biography

    Abu Freha Naim
    Ben-Gurion University, Israel

    I received my M.D. from the Tuebingen University, Germany at 2005 before becoming resident at internal medicine and then completed my gastroenterology residency at the Soroka Medical Center at 2014. I am employed as a senior physician at the Institute for Gastroenterology and Hepatology, Soroka University Medical Center and lecturer at the faculty of Health science, Ben-Gurion University, Beer-Sheva, Israel. I deal with gastroenterology and hepatology in general, and especially with genetic syndrome of the colorectal cancer. I researched different topics in the gastroenterology/Hepatology and different issues regarding the Bedouin Arab minority in southern Israel. I am one of the founders group of the Arab Medical Associations in the Negev (AMAN) and the first Chairman of the Associations since 2015.



    Abstract
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    Abstract

    Abu Freha Naim
    Ben-Gurion University, Israel

    The lecture will include a review of this medications group, including the history and development of the different type of proton pump inhibitors, the differences between the different PPIs in term of pharmacodynamic and pharmacokinetic features. Indications of treatments, efficacy of the drugs and the doses of the different PPIs and route of taking will be a part of the lecture. The PPIs were considered as safe medications, however in the last decade the are more and more reports regarding different side effects, in the short term and long-term use. The talk will include a comprehensive review of the different reported side effects in the updated literature. Important side effects will be included in the talk, osteoporosis, fracture risk, gastrointestinal infections, pneumonia, iron deficiency anemia, vitamin B12 deficiency, hypomagnesemia and increasing risk of polyps and gastric cancer. Another issue will be included in the lecture will be the updated recommendation regarding use of proton pump inhibitors during pregnancy and during breastfeeding. What about drug-drug interaction? and what about patients with chronic renal failure or liver cirrhosis? Should the dose be adjusted? These questions will be answered during the talk. The talk will be closed with the best practice advices regarding these drug group.

    Time:
    14:25-14:50

    Title: Involvement of Inflammation in the Pathophysiology and Treatment of Mood Disorders

    Abed N Azab
    Ben-Gurion University of the Negev, Israel

    Biography
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    Biography

    Abed N Azab
    Ben-Gurion University of the Negev, Israel

    Dr. Azab completed his Ph.D. in pharmacology in the Department of Clinical Pharmacology in Ben-Gurion University of the Negev (Israel), focusing on the study of anti-inflammatory drugs. Subsequently, as a post-doctoral fellow in Wayne State University (Michigan), Dr. Azab investigated the mechanisms of mood-stabilizing drugs, focusing on the role of GSK-3 in inositol biosynthesis. Currently, Dr. Azab is an assistant professor in Ben-Gurion University of the Negev. Major research projects in his lab: 1) Studying the role of inflammation in the pathophysiology and treatment of mood disorders; 2) searching for novel therapeutic strategies for mood disorders.



    Abstract
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    Abstract

    Abed N Azab
    Ben-Gurion University of the Negev, Israel

    Introduction. Accumulating data suggests that inflammation plays a role in the pathogenesis and treatment of mood disorders. Consistently, classic anti-inflammatory drugs exerted beneficial effects in randomized clinical trials of mood disorders patients. Moreover, psychotropic drugs possess anti-inflammatory effects. However, despite these supporting findings, many contradicting results have also been reported. Aims. (1) We investigated the effects of chronic treatment with psychotropic drugs on bacterial lipopolysaccharide (LPS)-induced inflammation in rats; (2) we tested the efficacy of mechanistically-different anti-inflammatory compounds in behavioral models in rats. Materials and Methods. (1) Rats were treated with psychotropic drugs (carbamazepine, lithium, haloperidol, and imipramine) for 4 weeks through a daily intraperitoneal (ip) injection. On day 29, rats were injected with vehicle or LPS. At 2 hours later, rats were sacrificed, blood was collected and different brain regions were excised. Levels of inflammatory constituents in plasma and brain were examined by ELISA. (2) Rats were treated (ip) for 2 weeks with one of the following anti-inflammatory compounds: dexamethasone, a potent corticosteroid; nimesulide, a selective cyclooxygenase-2 inhibitor; montelukast, a leukotrienes receptors antagonist; pentoxifylline, a tumor necrosis factor-inhibitor; and, JSH-23, a selective inhibitor of nuclear factor- B. At the end of drug treatment, animals were subjected to a battery of behavioral tests. Results. Psychotropic drugs treatment resulted in both anti- and pro-inflammatory effects. Some of the drugs prominently affected the levels of particular inflammatory mediators in the plasma and specific brain regions. Similarly, the various anti-inflammatory compounds differently affected the behavioral phenotypes of rats, showing both therapeutic and negative effects. Conclusions. Our results add to the ambiguity regarding the role of anti-inflammation as a therapeutic strategy for mood disorders. It is important to elucidate the therapeutic potential of mediator/pathway-specific anti-inflammatory compounds in randomized clinical trials.

    Time:
    14:50-15:15

    Title: Non-invasive NaV Measurements using Molecular Imaging

    Matthias Schoenberger
    University of Leuven, Belgium.

    Biography
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    Biography

    Matthias Schoenberger
    University of Leuven, Belgium.

    Matthias’ group in the pharmacy departmentperformsf KUinterdisciplinaryLeuven (Belgium research in Chemical Biology and Imaging with the goal of understanding ion channel dynamics in human disease.



    Abstract
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    Abstract

    Matthias Schoenberger
    University of Leuven, Belgium.

    Voltage gated sodium channels constitute an integral component of electrical conduction in the heart and the nervous system. By depolarizing cardiomyocytes or neurons, they initiate action potentials that underlie the electro-mechanical coupling and neuronal firing. Changes in cardiac NaV function or expression levels can have severe consequences. For example, patients with loss-of-function or gain-of-function mutations in the SCN5A gene, which decodes cardiac NaV1.5, are at risk of suffering sudden cardiac death due to ventricular arrhythmias. However, it has not been possible to measure NaVs in vivo due to lacking molecular probes or techniques. To fill this gap and enhance our understanding of in vivo NaV function, we have developed the first positron emission tomography (PET) radiotracer for NaVs –radiocaine. In this talk, I will present the development and in vivo characterization of radiocaine and discuss steps towards future translational perspectives.

    Time:
    15:15-15:40

    Coffee Break:15:40-15:55

    Title: Transforming Pharmacy Practice

    Allan Mathews
    Quest International University Perak, Malaysia

    Biography
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    Biography

    Allan Mathews
    Quest International University Perak, Malaysia

    Dr. Allan Mathews holds a B.Pharm (Hons) degree and post‐graduate qualifications in Clinical Pharmacy, Operations Management, Sales Management and Marketing. He is a registered pharmacist in Malaysia as well as a life‐member of the Malaysian Pharmaceutical Society. He also serves as a member of the Pharmacy Board of Malaysia. He has got 14 years of experience in Hospital/Clinical Pharmacy, 22 years in Industrial Pharmacy and 10 years in academia. He practices as a community pharmacist at the faculty‐run community pharmacy in addition to his current role as the Dean of the Faculty of Pharmacy, Quest International University Perak, Malaysia



    Abstract
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    Abstract

    Allan Mathews
    Quest International University Perak, Malaysia

    In ancient times, the pharmacist practiced side‐by‐side with the doctor in treating a patient giving the impression of equal responsibility bearing. The two professions were legally separated in the 13th century in Europe. The pharmacist retreated to the pharmacy and compounded medication according to the prescription received and dispensed to the patient carrying out instructions of the doctor. Compounding was the independent area of practice. With mass production of pharmaceutical dosage forms, the need for compounding became less and the pharmacist focused on dispensing of prescriptions expanding into prescription screening. Three main areas of practice evolved – community pharmacy, hospital/clinical pharmacy and industrial pharmacy. Community pharmacy evolved to a place where medicines are dispensed and became discount centers, shops selling items not related to healthcare and moving away from evidence‐based pharmacy. Countries vary in the extent of empowerment on the range of controlled medicines the pharmacist can deal without a prescription. Hospital/Clinical pharmacy evolved with the pharmacist being heavily involved in direct pharmaceutical care of patients while retaining custodianship of the medicine supply chain. Value creation and recognition continue to be a problem. The prescriber bears virtually the full responsibility. Prescribing by pharmacists has taken hold as well as expanded roles in administration of medicines and in medicine management. The challenge is how to develop independent areas of practice leading to responsibility bearing, value creation and recognition. Another challenge is to develop world‐wide commonality of practice. The focus of training of pharmacists should be to create full‐fledged practitioners rather than advisors.

    Time:
    15:55-16:20

    Title: Design and Synthesis of Ligands for G Protein-Coupled Receptor-1

    Thet-Thet Htar
    Monash University Malaysia, Malaysia

    Biography
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    Biography

    Thet-Thet Htar
    Monash University Malaysia, Malaysia

    Dr. Htar is an academic lecturer and researcher in School of Pharmacy, Monash University Malaysia. She graduated with Bachelor of Pharmacy (Honours) in 1999 from the University of Wales, Cardiff where she successfully completed her PhD in 2004. Her current research interest is in the area of drug design and synthesis of small molecules with potential anti-cancer activity for the treatment of breast cancer. Her research interest has been extended to chemistry of natural products and synthesis of natural products based analogues. She has published a number of research and review articles in the area of synthesis and natural products.



    Abstract
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    Abstract

    Thet-Thet Htar
    Monash University Malaysia, Malaysia

    G protein-coupled estrogen receptor-1 (GPER-1, formerly known as GPR30) is a novel membrane receptor, which is noted to be able to mediate rapid estrogen signaling. It is widely expressed in cancer cell lines and primary tumour of breast, endometrium, ovaries, thyroid, lung, prostate, testicular germ cells and the brain. The high level expression of GPER-1 in breast cancer, ovarian cancer and endometrial cancer has shown association with poor survival. Due to its significant association with numerous physiological functions and breast cancers, it is emerging as a potential therapeutic target. In the search of GPER ligands, a variety of classical ER ligands have been noted to be able to interact with GPER-1 with differences in binding affinities. Some of the traditionally known ER antagonists have shown agonistic activity with GPER protein. As X-ray crystal structure GPER protein is not available, it has been a challenge in designing of GPER ligands. In this project, we studied the molecular structure requirement of GPER-1 ligands using homology model of GPER. We reported here approaches that have been used in homology modeling of GPER protein and synthesis of potential GPER ligands.

    Time:
    16:20-16:45

    Title: Pharmacology of Endothelium and Vascular Smooth Muscle in Vascular System: The Comparison of Human Internal Mammary Artery (IMA) and Rat Aorta (RA)

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Biography
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    Biography

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Dr Arslan is head of pharmacology department in the Ankara Yildirim Beyazıt University. His researches are focus on the experimental pathopysiology and inflammation of pulmonary and cardiovascular systems. He is PhD supervisor for pharmacology or toxicology students



    Abstract
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    Abstract

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Vascular smooth muscle (VSM) and endothelium (ET) activities are the important determinants of vascular tone. This presentation is to evaluate the regulatory functions of VSM and ET in human internal mammary artery (IMA) and rat aorta (RA). Potassium (K+) channels contribute to the regulation of the membrane potential in VSM cells. Membrane hyperpolarization due to efflux of K+ results from the opening of K+ channels and in final, vasodilation can be occurs. Endogenous and exogenous various substances which form contraction and relaxation alter vascular tone by interacting with their receptors in the VSM and ET. The contraction and relaxation responses of the IMA and RA can be analyzed in vitro organ baths. Acetylcholine (ACh) produces endothelium-mediated relaxation while potassium chloride (KCl), phenylephrine (PE), and 5-hydroxytryptamine (5-HT) produce VSM-mediated contraction. ET-denuded vessels do not relax sufficiently by acetylcholine. ACh relaxes the ET-intact IMA and RA, it does not relax the ET-denuded IMA and RA. The KCl-induced contractions are more stable and longtime than those of the PE and 5-HT. However, the percentage of the PE and 5-HT induced contractions bigger than that of KCl. ET and VSM play important role in relaxation and contractions mechanisms of the IMA and RA. The physiological characteristics of IMA and RA in the formation of vascular tone highly look like similar. Different substances which occur relaxation and contraction causes to varied responses in these vessels.

    Time:
    16:45-17:10

    Title: Effects of Bulgarian Propolis on Cd34+ Cells in Vivo And on two Cell Lines in Vitro

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria

    Biography
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    Biography

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria

    Professor Dr. Lyudmil Peychev Peychev, PhD, MHM, is Dean of the Faculty of Pharmacy at the Medical University of Plovdiv, Bulgaria and Head of the Department of Pharmacology and Drug Toxicology. He is a specialist in Pharmacology, Clinical Pharmacology and Therapeutics, Master of Health Management. Professor L.Peychev is the author of scientific articles in the field of neuropharmacology, clinical pharmacology, phytotherapy and apitherapy. He is Head of research projects and author of inventions and trademarks. As a scientist he has won recognition by the academic community in Bulgaria and other countries.



    Abstract
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    Abstract

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria

    Purpose. To evaluate whether Bulgarian propolis augment the release of hematopoietic stem cells from bone marrow into the blood stream of rats and to analyze its effect on cell proliferation and surviving in vitro. Methods. Twenty male Wistar rats were divided into two groups (n=10) and treated as follows:1-st group (Controls) – aqua destillata, p.o.; 2-nd group – Bulgarian propolis in a dose 100 mg/kg bw, i.p. Two hours after single administration of the substances blood samples were analyzed by flowcytometry for CD34+ and white blood cells differential were performed using hematology analyzer. Peripheral blood mononuclear cell (PBMC) and Mouse lymphoma cell line L5178Y were cultivated in 96 well plate in culture medium RPMI-1640 with 100 U/mL Penicillin and 100 mg/mL Streptomycin. 10% Fetal bovine serum was added to the L5178Y cell line. The cells were grown in an incubator at 37оС and 5% СО2 for 24 hours and were treated with increasing propolis concentrations - 0,01; 0,1; 1,0 and 10 mg/L. To analyze the effect of the natural product on cell viability, a cytotoxic MTT-test was used. Results. Propolis mobilized significant amount of hematopoietic stem cells in the blood stream versus controls. It also caused a significant increase in the number of leukocytes and lymphocytes in rats in comparison to controls. In human PBMC cells treated with propolis in concentrations 0,01; 0,1; 1,0 and 10 mg/L the cell vitality was higher in comparison with the control cells. The percentage of living cell was increased from 106 to 135% with increasing of the propolis concentrations. In propolis treated tumor cells L5178Y the percentage of living cells decreased with increasing of propolis concentrations. The results of 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MMT) assay showed that concentration 0,1 mg/L leads to 73% cell survival, 1,0 mg/L tо 69%, and the highest concentrations - 10 mg/L decreased the percentage of surviving cells to 40% . Conclusion. Bulgarian propolis has no effect on the stem cells maturation but mobilize the rats stem cells in peripheral blood. In human PBMC the proliferative activity after treatment with propolis suppose the ability to influence cell differentiation. From the other side the cytotoxic effect of propolis on the tumor cell line determines its potential in anti-tumor therapy. Keywords: CD34+ cells, cell lines, proliferation, rats, Bulgarian propolis.

  • Keynote Speaker

    Time:
    10:00-10:30

    Title

    Title: Ovarian Cancer Diagnosis Through Genome-scale Expression

    Antonio Scilimati
    University of Bari, Italy
    Biography
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    Biography

    Antonio Scilimati
    University of Bari, Italy

    Antonio Scilimati graduated cum laude in Chemistry at the University of Bari (Bari, Italy). PhD at the University of Wisconsin (Madison, USA). Four years as a Qualified Person at MerckSerono plant producing recombinant drugs. Currently, he is an Associate Professor of Medicinal Chemistry at the University of Bari. His scientific interest focus the "pharmaceutical sciences", targeting the cyclooxygenase (COX)-1 as a novel theranostic biomarker in oncology and neuroinflammation.



    Abstract
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    Abstract

    Antonio Scilimati
    University of Bari, Italy

    Epithelial ovarian cancer (EOC) is the most lethal gynaecologic malignancy. The global EOC burden is approximately 225,000 new cases per year, with a survival rate of 30%. EOC is hallmarked by a high degree of heterogeneity. This heterogeneity is apparent in tumor histopathology such as serous, mucinous, endometrioid and clear cell histotypes. The high-grade serous ovarian cancer (HGSOC) accounts for approximately 70% of all ovarian carcinoma. Patients with HGSOC show diverse clinical outcomes and usually low survival rates (LSRs), even after the same or very similar treatment regimens. This LSR would also be ascribed to the diagnosis usually made in advanced stage, because no or specific symptoms are related to EOC onset and progression. Early diagnosis determines the patient overall survival. Currently, CA 125 and HE4 are used as early stage EOC biomarkers. Unfortunately, they are not EOC specific and have a poor diagnostic rate, so that the EOC biomarker research is still a challenge. In this presentation, we will show a comparison between the transcriptome profiles of hundreds of HGSOCs and normal tissues, obtained from RNAseq experiments and reported in big data portals. Our analysis resulted in a panel of tenths of genes that are strongly over-expressed in cancer tissues and might be novel candidates as cancer biomarkers. The expression of our selected genes was further tested on a different cohort of patients by comparing matched cancer and normal tissues by the nano-string technology to validate a panel of biomarkers suitable for a very precocious EOC diagnosis. This project is granted by First AIRC Grant-MFAG2015 (Project Id. 17566).

    Keynote Speaker

    Time:
    10:30-11:00

    Coffee Break: 11:00-11:15

    Title

    Title: Development of Injectable Drugs to Improve the lifetime and Biocompatibility of Prosthetic Articular Joints

    Seunghwan Lee
    Technical University of Denmark, Denmark
    Biography
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    Biography

    Seunghwan Lee
    Technical University of Denmark, Denmark

    Dr. Seunghwan Lee completed PhD in physical chemistry in 2000(University of Houston), then worked in the field of biotribologyand biomimetic lubrication at ETHZ, Switzerland until 2008.Since 2009, he has been leading a research group focusing on biotribology of mucin, mucus, orthopaedic implants, and antifouling properties at the Department of Mechanical Engineering, DTU, Denmark.



    Abstract
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    Abstract

    Seunghwan Lee
    Technical University of Denmark, Denmark

    Wear problems of bearing materials for prosthetic articular joints, such as total hip arthroplasty (THA) or total knee arthroplasty (TKA),have long been recognized as a major cause of degradation and failure of the implants. Thus, improvement of anti-wear properties of implant materials is a key requirement to improve lifetime of prosthetic articular joints. To date, efforts to solve this problem have been directed towards the development and application of new materials with superior anti-wear properties. The present study proposes to solve this problem by administering external lubricants to prosthetic articular joints. This approach is primarily based upon recent development of various lubricant additives that improve anti-wear properties even in aqueous environment. With an aim to reduce the wear of ultrahigh molecular weight polyethylene (UHMWPE), external lubricants were formulated by dissolving commercial amphiphilic copolymers in aqueous buffer solution. Tribological studies have shown that the tested copolymers displayed immediate reduction effects in the coefficient friction upon injection for the sliding contacts between CoCrMo pin and UHMWPE disk in calf serum as model synovial fluid.For in-vitro cytotoxicity tests, cell morphology and standard MTT assay on murine fibroblast and osteoblast showed a positive result.Near future studies pursue to investigate the impact on wear properties of UHWMPE and extended biocompatibility. Ultimately, it is the aim to develop drugs in the form of pre-filled syringe to improve lifetime and biocompatibility of prosthetic articular joints.

    Keynote Speaker

    Time:
    11:15-11:45

    Title

    Title: Importance of Pharmacogenomics in the Success of Drug Therapy

    David W. Hein
    University of Louisville, USA
    Biography
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    Biography

    David W. Hein
    University of Louisville, USA

    Dr. Hein serves as Peter K. Knoefel Endowed Chair of Pharmacology, Professor and Chairman of the Department of Pharmacology & Toxicology, and Distinguished University Scholar at the University of Louisville (USA). His research program includes studies of the molecular epidemiology of cancer susceptibility, pharmacogenetics, genomics, personalized medicine, and functional genomics. He has coauthored over 240 peer-reviewed journal articles and book chapters, 75 published gene sequences, and over 600 abstracts. The publications have over 13,000 citations with an h-index 58. He has served as principal investigator/co-investigator/mentor on over 75 research grants and contracts totaling over $50 million dollars.



    Abstract
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    Abstract

    David W. Hein
    University of Louisville, USA

    Individual patient differences in drug responsiveness are well recognized by health care professionals. Understanding the basis for these differences is of major clinical and economic importance because of the high frequency of both therapeutic failure and adverse reactions to drugs. Patients may receive inadequate or suboptimal benefit and/ or suffer adverse effects from drug treatment. In this presentation, we highlight pharmacogenetic/pharmacogenomic principles and provide illustrative examples where these principles can be applied to optimize therapeutic benefit and minimize adverse effects.

    Sessions:
    Analytical and Bioanalytical Techniques & Protien Protien Interactions on Drug Targets

    Time:
    11:45-12:10

    Title: Analysis of Isoniazid and Acetylisoniazid to Determine the Acetylation Rate of Melanesian Ethnicity from Papua

    Yahdiana Harahap
    University of Indonesia, Indonesia

    Biography
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    Biography

    Yahdiana Harahap
    University of Indonesia, Indonesia

    Dr. Yahdiana Harahap is a Professor in the field of Pharmaceutical Chemistry especially Bioanaysis related to bioequivalence study and DNA Adduct. She received her Master Degree in 1994 and Doctoral degree in 2003 from Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Institute Teknologi Bandung. She has been The Head of Bioavailability-Bioequivalence Laboratory Faculty of Pharmacy Universitas Indonesia since 2008. She has generated more than 80 scientific works published in international and national journals, thus presented them in national and international conferences.



    Abstract
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    Abstract

    Yahdiana Harahap
    University of Indonesia, Indonesia

    The major pathway of isoniazid (INH) metabolism is by acetylation to form acetylisoniazid (AcINH). The acetylation rate may differ depending on N-Acetyltransferase-2 (NAT-2) activity. The aim of this research was to determine INH acetylation rate on Melanesia ethnicity from Papua, Indonesia. The rate of acetylated INH was determined based on the ratio of AcINH and INH levels in human plasma which was analysed using HPLC. The chromatographic separation was conducted using Reliant C18 column (250x4.6mm) with temperature of 30C, a 20 mM hexane sulphonic acid pH 2.47-methanol (65:35) as mobile phase with a flow rate of 1 mL/mins, detected at 265 nm, and vitamin B6 as an internal standard. The participants in this study were healthy subjects from Papua and 102 subjects completed the study. The blood was collected at pre-dose and 3 hours after administration of 300 mg INH. The results showed that 70.59% of subjects were slow acetylators, it means that the Melanesia ethnic from Papua need more dosages to achieve the therapeutic concentration of INH.

    Time:
    12:10-12:35

    Title: Kinetic Target-Guided Synthesis: A MS-Based Fragment Evolution Platform

    Roman Manetsch
    Northeastern University, USA

    Biography
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    Biography

    Roman Manetsch
    Northeastern University, USA

    Roman Manetsch received his PhD degree in 2002 from the University of Basel (Switzerland) working with Professor Wolf-Dietrich Woggon. After a postdoctoral experience with Professor K. Barry Sharpless at the Scripps Research Institute, he started his independent career as an Assistant Professorat the Department of Chemistry at the University of South Florida (Tampa, Florida). In 2014, Associate Professor Manetsch moved to theDepartment of Chemistry and Chemical Biology and the Department of Pharmaceutical Sciences at Northeastern University (Boston, Massachusetts). His current research focuses on the development offragment-based lead discovery strategies, the development of chemical probes for the study of specific proteins in complex biological matrices, as well as hit-to-lead optimizations of anti-malarial, anti-leishmanial, and anti-bacterial agents.



    Abstract
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    Abstract

    Roman Manetsch
    Northeastern University, USA

    The Manetsch laboratory focuses on the development and implementation of LC/MS-based lead discovery and optimization platforms. Herein, the development of kinetic Target-Guided Synthesis (TGS) and its implementation for the identification of small molecules modulating protein-protein interactions will be presented. In kinetic Target-Guided Synthesis (TGS), the biological target is actively engaged in the irreversible assembly of its own inhibitory bidentate ligand from a pool of smaller reactive fragments. The screening method can be as simple as determining whether or not the inhibitory product has been formed in a given test mixture. To date, kinetic TGS has exclusively been applied to enzymatic targets and these TGS applications have been successful because of a unique combination of (a) the slow nature of the chemical reaction combining the two fragments into a single molecule and (b) the use of reactive fragments showing moderate to high affinity towards binding pockets of the enzyme. Compared to kinetic TGS screening of enzymes, however, the discovery of inhibitory compounds against "undruggable" targets is more challenging and thus requires major modifications over the existing kinetic TGS approaches. The Manetsch laboratory demonstrated that the sulfo-click reaction, an amidation reaction between thio acids and sulfonyl azides, is suitable for a kinetic TGS approach targeting the proteins of the Bcl-2 family. Furthermore, the use of a triple quadrupole mass analyzer improved the data quality and increased the throughput by approximately 200-fold rendering the platform industrial robustness.

    Time:
    12:35-13:00

    Lunch Break 13:00-14:00

    Title: Scaffolds Based on Electrospun Nanofibers for Wound Healing

    Giuseppina Sandri
    University of Pavia, Italy

    Biography
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    Biography

    Giuseppina Sandri
    University of Pavia, Italy

    Sandri Giuseppina (PhD in Chemistry and Pharmaceutical Technology) is Associate Professor at Drug Science Department (Faculty of Pharmacy) at University of Pavia, Italy. Current research concerns the development medical devicesand antimicrobials based formulations based on electrospun nanofibers for skin and hearth regeneration. Her research activities were awarded with national and international awards and were founded with public and private grants. She takes part in joint research projects with Universities andpharmaceutical companies. She serves as reviewer for several scientific journals. Total number of referred publications:86 contributions published on scientific journals, 12 book chapters, 3 patents;Hindex:25 (Scopus).



    Abstract
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    Abstract

    Giuseppina Sandri
    University of Pavia, Italy

    Electrospinning is a one-step and simple method to manufacture membranes based on (nano)fibers having diameters ranging from 20 nm up to 1m and more. Scaffolds based on nanofibrous membranes could allow protecting chronic skin lesion them from microbial contamination and optimal wound hydration and gas exchanges, crucial for healing, and should be a substrate able to induce cell adhesion and growth, speeding up the healing process. Given this premises the aim was the development of electrospun nanofibers based membranes as scaffolds to enhance cutaneous wound healing of chronic lesions and burns. The nanofibers were prepared starting from aqueous polymeric solutions to obtain insoluble membranes in aqueous fluids able to act as a support for cell growth, migration and proliferation. Nanofibers were loaded with either silver nanoparticles ornorfloxacin, as antimicrobial agents. Chitosan and a glycosaminoglycan (hyaluronic acid or chondroitin sulfate)wereselected as biomaterials. The developed scaffolds based on biopolymers are characterized by flexible area and shape with suitable elasticity and mechanical properties, are able to maintain optimal hydration and to absorb excess of fluids, to form barrier against microbial contaminations and to release antimicrobial agents. The presence of antimicrobials agents do not impair cell adhesion (fibroblasts and endothelial cells) and proliferation in an in vitro models. The in vivo evaluation in murine burn model suggests that scaffolds are effective to enhance wound closure without signs of inflammation and adverse effects.

    Sessions:
    Novel Drug Delivery Systems & Drug Discovery and Development

    Time:
    14:00-14:25

    Title: Development and Efficacy Evaluation of Topical Preparations for Nail Disorders

    Paola Perugini
    University of Pavia, Italy.

    Biography
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    Biography

    Paola Perugini
    University of Pavia, Italy.

    Prof. Perugini, graduated in Pharmaceutical Technology and Chemistry (CTF) and in Pharmacy, earned her PhD in Pharmaceutical Technology and Chemistry in 1998. Now she is an associate Professor at the University of Pavia in which she teaches in Pharmacy and in CTF degree courses. Furthermore she is the Coordination of the Master Degree in "Cosmetological Sciences" at the University of Pavia from 2009-2010. The main research activities of Prof. Perugini concerning pharmaceutical and cosmetic technology. The scientific work of Dr. Perugini has resulted in over 60 publications, 2 patents, more than 100 communications on topics of technology pharmaceutical and cosmetics.



    Abstract
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    Abstract

    Paola Perugini
    University of Pavia, Italy.

    This presentation proposes an overview of the development and testing of effective treatment for nail disorders, in particular onychodystrophy that is an alteration of the tropism of the nail, due to systemic and local causes. Topical treatment avoids adverse effects associated with systemic therapy. However, the effectiveness of topical therapies is limited because of the poor permeability of the lamina to the therapeutic agents applied. In fact, it must be borne in mind that the nail is basically a tortuous twist of crystalline and amorphous fibrous proteins in which only small molecules pass through. In recent years the research has mainly focused on improving transdermal permeability by chemical treatments, penetration enhancers, mechanical and physical methods. For this purpose, several "ex vivo" methods have been studied, including the use of bovine membranes as a human nail substitute for penetration studies. The aim of recent works was to evaluate whether and to what extent such in vitro tests can correctly predict the in vivo fate of nail lacquers. In the application of pharmaceutical lacquers used to treat nail alterations and fungal infections, the location of the film on the nail lamina and the transungueal passage of the active substance are the key factors. Hence the importance of developing topical products with appropriate formulation both in terms of medical treatment and associated cosmetic treatment. In fact, the constant use of an inappropriate cosmetic formulation can severely compromise the effectiveness of medical treatment.

    Time:
    14:25-14:50

    Title: Hepatoprotective Properties of the Salt-Like Derivatives of the Drug Xymedon with Biogenic Acids

    Vyshtakalyuk A B
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Biography
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    Biography

    Vyshtakalyuk A B
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Alexandra Vyshtakalyuk is a senior researcher, Doctor of biology in the International research and innovation center of neurochemistry and pharmacology of A.E. Arbuzov Institute of Organic and Physical Chemistry - Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of Russian Academy of Sciences", Kazan, Russia. She graduated from Kazan State University in 1994, got PhD in Physiology of Human and Animals in 2000 and degree of Doctor in biology in 2015. She is head of the small team that studies hepatoprotective, anti-anemic and anti-inflammatory properties of new compounds. Her science interest is in the area of pharmacology, immunology, hematology and metabolism.



    Abstract
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    Abstract

    Vyshtakalyuk A B
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    The objective of this work was an evaluation of hepatoprotective activity of derivatives of drug Xymedon (1-(ß-oxyethyl)-4.6-dimethyl-1.2-dihydro-2-oxopirimidon) (I) with biogenic acids succinic, L-ascorbic, para-aminobenzoic, nicotinic, L-2-amino-4-(metyltio)butane (L-methionine) (II-VI respectively).Formerly, we have shown that (I) possesses ability to stimulate liver recovery after toxic damage by CCl4 [1]. New data are on derivatives (III, IV) that have a better specific capability if compared with (I) to stimulate spinal marrow recovery after trauma [2], improve body's adaptation under physical stress [3, 4], as well as demonstrate hepatoprotective properties [5-7].We observed the most pronounced hepatoprotective properties of derivative (III) [6], because the compound results to minimum liver injury of steatosis and necrosisand normalize of biochemical parameters in rats with toxic hepatitis induced by CCl4. It was revealed the effect of the compound (III) on the antioxidative system and on the cytokines level in liver during toxic influence of CCl4. The number of pathological changed hepatocytes as fatty, balloon and hydropic dystrophy were decreased if injected with (III). The derivative (III) is more effective in comparison with other derivatives ofXymedon as well as with Xymedon, L-ascorbic acid, their mix and their co-administration and the drug Thiotriazolinum.

    Time:
    14:50-15:15

    Title: In Vitro Permeation and Pharmacokinetic Evaluations of a Novel Transdermal Formulation of CZ48, Lactone-Stabilized Camptothecin-C20-propionate, for Cancer Treatment

    Yousif Rojeab
    Ohio Northern University, USA.

    Biography
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    Biography

    Yousif Rojeab
    Ohio Northern University, USA.

    Dr. Rojeab is currently an Associate Professor of Pharmaceutics at the Raabe College of Pharmacy, Ohio Northern University in Ada, OH, USA. He received his B.Sc. in Pharmacy in 1999 and Ph.D. in 2007 in Pharmaceutics from University of Houston, TX. He is also a licensed pharmacists in the states of OH, MI & TX. Current research in Dr. Rojeab’s lab involves two main areas. First is the physicochemical characterization, preformulation, formulation and delivery of anti-cancer agents. The second research area involves bioavailability/bioequivalence evaluations of novel and conventional orally administered solid dosage forms in man.



    Abstract
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    Abstract

    Yousif Rojeab
    Ohio Northern University, USA.

    Camptothecin (CPT) is a promising anti-cancer agent with high efficacy against a variety of solid tumors. The lactone ring of CPT is essential for the anti-tumor activity but is unstable in vivo. A pro-drug of CPT, CZ48, has been developed where CZ48 is enzymatically hydrolyzed in vivo to active CPT. For its anti-cancer activity, CPT levels need to be sustained and maintained over a period of time, which means the requirement for a continuous delivery of CZ48. It has been demonstrated that I.V. bolus injection of CZ48 provided higher concentrations of the active CPT compared to direct dosing of CPT at the same dosing level in Sprague-Dawley rats. Also, it was shown that CZ48 dosing resulted in sustained plasma levels of CPT for five hours in this animal model. Therefore, if we extrapolate these observations to man, we would expect to see this pattern of sustained CPT levels upon administration of the pro-drug, CZ48. This means delivery of CZ48 can achieve a continuous, therapeutically relevant drug concentration of CPT for anti-neoplastic indications. Transdermal delivery satisfies the requirement for continuous CZ48 delivery since it allows for predictable and controlled drug permeation through the skin. A promising approach to deliver drug molecules transdermally is through a microemulsion formulation. Our in vitro permeation data through excised rat skin as well as in vivo data upon topical application in Swiss-nude mice demonstrate the feasibility of achieving sustained plasma levels of CPT, required for anti-tumor activity, upon transdermal application of the pro-drug, CZ48.

    Time:
    15:15-15:40

    Title: Nanotechnology-based Drug Delivery to Skin

    Fiorenza Rancan
    Freie University Berlin, Germany

    Biography
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    Biography

    Fiorenza Rancan
    Freie University Berlin, Germany

    Fiorenza Rancan is private lecturer and associated scientist in the Clinical Research Center for Hair and Skin Science at the Dermatology Department of the Charité – Universitätsmedizin Berlin. She earned a doctorate in Chemistryfrom the Humboldt University of Berlin and adegree in Pharmaceutical Chemistry and Technology from the University of Padua, Italy.Dr. Rancan expertise lays ondermal and transdermal drug delivery. Her main research topics are the development of new antimicrobial treatments using skin models for infected wounds,antigen and adjuvant delivery for transcutaneous vaccination, stimuli-responsive nanocarriers for the treatment of skin inflammatory conditions.



    Abstract
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    Abstract

    Fiorenza Rancan
    Freie University Berlin, Germany

    Nanotechnology-based carrier systems allowimproving drug permeation,spreading within the tissue, retention, and even targeted and stimuli-responsive drug delivery. In skin inflammatory diseases, immune cells are the main key actors and thus the target of therapeutic treatments. Targeting of certain cell populations like dendritic cells may be achieved by means of biocompatible nanocarriers that can translocate to the skin viable layers. Thermoresponsive polyglycerol-based nanogels (tNGs) have been shown to penetrate very efficiently the outermost skin barrier and to be valuable tools for delivery of drugs to skin. In order to develop new types of formulations for anti-inflammatory dermatotherapy,tacrolimus, a high molecular weight, poorly skin penetrating drug, was loaded onthermoresponsive nanogels.Compared toa commercial formulation (Protopic 0.1%),the water-based nanogel formulation had comparable effects on an inflammatory skin model.On the other side, in the case of skin and wound infections, high local concentrations of the active materialare necessary to eradicate the invading microorganism and avoid the development of resistances.At this purpose, we used PVP-based foils and nanofibers to deliver antimicrobial drugs in an infected wound model based on ex vivo human skin. Different delivery profiles were achieved depending on the useddrug delivery system. Nanocarriers efficiently delivered drugs across the skin barrierwith significant inhibitory effects on immune cells whereasnanofibers and foils provided a sustained drug concentration within the skin tissue as well as very efficient antimicrobial effects. We conclude that nanotechnology-based drug delivery systems offerattractive alternatives to conventional drug formulations.

    Time:
    15:40-16:05

    Coffee break 16:05-16:20

    Title: Activation of the AIM2 Inflammasome in Peripheral Blood Mononuclear Cells (Pbmcs) From Idiopathic Pulmonary Fibrosis Patients Leads to the Release of Pro-Fibrotic Mediators

    Rosalinda Sorrentino
    University of Salerno, Italy

    Biography
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    Biography

    Rosalinda Sorrentino
    University of Salerno, Italy

    Dr. Rosalinda Sorrentino, PhD, is actually a Tenure Track Research Scientist at the University of Salerno. During her PhD training, Dr. Sorrentino joined Prof. Jane Mitchell’s laboratory at Imperial College-NHLI (UK) as a visiting scientist. In 2007 Dr. Sorrentino obtained a PostDoctoral Position at Cedars Sinai Medical Center, USA. In 2008 she moved to Italy to establish her own research project to understand how chronic inflammation in the lung favors malignancy.



    Abstract
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    Abstract

    Rosalinda Sorrentino
    University of Salerno, Italy

    Idiopathic pulmonary fibrosis (IPF) is a chronic fibro-proliferative disease characterized by poor prognosis, with a mean survival of ~2–3 years after definite diagnosis. The cause of IPF is still unknown but it is a heterogeneous condition in which the aberrant deposition of extracellular matrix leads to extensive lung remodeling. This remodeling is a consequence of inflammatory responses, but the mechanisms involved are poorly understood. In this study, we first analyzed a bleomycin-induced mouse model, which showed that higher expression of IL-1β, but not IL-18, was correlated to pulmonary cell infiltration and fibrosis. Then, we found that peripheral blood mononuclear cells (PBMCs) from IPF patients released higher levels of IL-1α and IL-18 in a NLRP3- and calpain-independent manner after LPS±ATP. Instead, the activation of the AIM2 inflammasome induced the release of IL-1α in a caspase-1/-8 independent manner; whereas IL-18 release was caspase-1-dependent. These effects correlated with the release of the pro-fibrotic TGF-β, which was induced by AIM2 activation in a caspase-1- and TLR4-independent manner, but dependent on IL-1α. In this context, the activation of AIM2 induced the release of caspase-4 from IPF-derived PBMCs, which correlated with the mRNA levels of this caspase that was higher in IPF than in healthy PBMCs. In conclusion, our findings identify a novel molecular mechanism whereby the activation of AIM2 could lead to the activation of the non-canonical inflammasome (caspase-4-dependent), that induces the release of IL-1α responsible for the release of TGF-β from PBMCs of IPF patients.

    Time:

    Title: Penetration of Microparticles across Mucus Barriers: A Colloidal Probe Microscopic Approach

    SeunghwanLee
    Technical University of Denmark

    Biography
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    Biography

    SeunghwanLee
    Technical University of Denmark

    Dr. Seunghwan Lee completed PhD in physical chemistry in 2000(University of Houston), then worked in the field of biotribology and biomimetic lubrication at ETHZ, Switzerland until 2008.Since 2009, he has been leading a research group focusing on biotribology of mucin, mucus, orthopaedic implants, and antifouling properties at the Department of Mechanical Engineering, DTU, Denmark



    Abstract
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    Abstract

    SeunghwanLee
    Technical University of Denmark

    Mucus represents “first defense line” of all mucosal surfaces, such as gastrointestinal, cervical, ocular, respiratory lines against chemical, enzymatic, microbial, and mechanical insult. While pathogenic colonization by microbes starts from adhesion on mucus surface, they have to overcome the mechanical integrity of mucus gels in order to reach the apical surface of epithelial cells. Ironically, this defensive behavior of mucus gels acts as physical barrier when it is needed to deliver drug molecules or functional foods to target cells or tissues. Mucoadhesive polymers have been proposed as one of potent ways to enable the traverse of drug-loaded particles through mucus gel layers based on strong entanglement with mucin network. Nevertheless, it is often not sufficient to reach ultimate goal, i.e. delivery of drugs to target cells, due to relatively short turnover time and frequent clearance of mucus gels. It is thus necessary to develop more effective means to engineer particles that can efficiently “penetrate through” the barrier for this purpose. In this study, we have employed atomic force microscopy (AFM) as an experimental approach/model to characterize the nanomechanical properties of mucus gels and the interaction with microbes and drug/function nutrients. Mucus was acquired by scraping from a freshly slaughtered pig’s stomach and intestine, and was further cleaned according to a standard procedure. Polyethylene (PE) or silica (SiOx) colloidal particles were attached to AFM probes, representing model microbes or drug molecules interacting with mucus layers. Young’s moduli of both mucus samples were determined to be in kPa range. In-depth discussion on the mechanical properties of pig intestine mucus, the influence of size and surface chemistry of the colloidal probe on the compression/penetration, as well as the force/energy required for a micro-sized particle to overcome and penetrate through the mucus layers will be provided.

    Time:
    16:45-17:10

    Title: Organolithium Chemistry Using Flow Microreactors

    Aiichiro Nagaki
    Kyoto University, Japan

    Biography
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    Biography

    Aiichiro Nagaki
    Kyoto University, Japan

    Aiichiro Nagaki has received his Ph.D. in 2005 from Kyoto University under the supervision of Professor Jun-ichi Yoshida. He worked with Professor Hiroaki Suga, Tokyo University from 2005 as a postdoctoral fellow. In 2006, he became an assistant professor of Kyoto University. In the year 2013 he promoted to Junior Associate Professor. His current research interests are organic synthesis, polymer synthesis, and microreactor synthesis. Awards: Takeda Pharmaceutical Co., Ltd. Award in Synthetic Organic Chemistry, Japan (2012), Incentive Award in Synthetic Organic Chemistry, Japan (2012), and Young Innovator Award on Chemistry and Micro-Nano Systems (2013).



    Abstract
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    Abstract

    Aiichiro Nagaki
    Kyoto University, Japan

    Many successful applications reported in the literature speak well for the power of the flow-microreactor method in chemical synthesis. The reaction time in a flow microreactor is defined as the residence time between a reagent inlet and the quencher inlet, which can be controlled precisely and reduced to millisecond order by adjusting the length between these positions and the flow speed. Such a feature of flow microreactors enables the use of short-lived highly reactive intermediates for synthesis. Various intermediates can be rapidly generated and transferred to another location in the flow system for use in subsequent reactions before they decompose. Protecting-group-free synthesis has received significant recent research interest in the context of ideal synthesis and green sustainable chemistry. In general, organolithium species react with electrophilic functional groups very rapidly, and therefore such functional groups should be protected before an organolithium reaction, if they are not involved in the desired transformation. If organolithium chemistry could be free from such a limitation, its power would be greatly enhanced. A flow microreactor enables such protecting-group-free organolithium reactions by choosing the appropriate residence time and the reaction temperature. Organolithium species bearing alkoxycarbonyl, nitro, ketone and aldehyde carbonyl groups can be generated by lithiation of the corresponding organic halides and reacted with various electrophiles using a flow microreactor system. In this presentation, we report that a flow microreactor system enables the generation of various unstable organolithium compounds.

    Time:
    17:10-17:25

    Title: New Discovery of Treatment of Complicated Hemorrhoids without Surgery

    Ahmed M A Masaad
    Taif University, Saudi Arabia

    Biography
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    Biography

    Ahmed M A Masaad
    Taif University, Saudi Arabia

    Dr. Ahmed M. A. Massad is concern with discovery of new drugs and innovation in many new drugs and drug delivery system. Dr. Ahmed has memberships in many scientific societies and editor of PSC journal. He published more than twenty scientific researches in many sides of pharmacy, he discovered new treatment of cancer of colon and skin, possess innovative certificates in drugs delivery system and new drugs which are published in scientific journal.



    Abstract
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    Abstract

    Ahmed M A Masaad
    Taif University, Saudi Arabia

    Hemorrhoids, also called piles, are vascular structures in the anal canal. In their normal state, they are cushions that help with stool control. They become a disease when swollen or inflamed; the unqualifiedly term "hemorrhoid" is often used to refer to the disease. The signs and symptoms of hemorrhoids depend on the type present. Internal hemorrhoids are usually present with painless, bright red rectal bleeding when defecating. External hemorrhoids often result in pain and swelling in the area of the anus. If bleeding occurs it is usually darker. The new treatment is mainly depend on mechanism of contract the connective tissue surrounding the venous around anus by effervescent tannin base with strong anti-bacterial, antifungal and anti-viral effect of formula. The safety of drugs was tested in rabbits, rats first and then the experiment was done in hundreds of patient under license of ethics committee of Taif University. The percentage of cure conducted was 99%, this success leads to relive of pain over millions of patients around the world and minimize the risk of surgery treatment and cost beside quick relive of disease in two to three weeks with no chance of relapse of disease again.

  • Sessions:
    Pharmaceutical Chemistry & Pharma Applications in Microbiology and Biotechnology & Clinical Pharmacy & Biopharmaceutics and Pharmaceutics & Nutraceuticals

    Time:
    10:00-10:25

    Title: Antibacterial Activity of Ethanolic Extract and Nanoparticles Ethanolic Extract of Javanese Turmeric Rhizome (Curcuma Xanthorrhiza Roxb.)

    Shirly Kumala
    Pancasila University, Indonesia

    Biography
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    Biography

    Shirly Kumala
    Pancasila University, Indonesia

    Shirly Kumala has completed her PhD from Biomedical Faculty, University of Indonesia, Jakarta. She is the Dean of Pharmacy Faculty, Universitas Pancasila, Jakarta, Indonesia. She has published more than 25 papers both International and national journals, and has been serving as a reviewer in Journal of Pharmacy.



    Abstract
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    Abstract

    Shirly Kumala
    Pancasila University, Indonesia

    The aim of this study was to compare the antibacterial activity of 96% (v/v)ethanolic extract of Javanese turmeric(Curcuma xanthorrhizaRoxb.) and nanoparticle containing the extract against4 pathogenic bacterias. The method to formulate the nanoparticles extract was ionic gelation using chitosan and sodium tripolyphosphate. The resulting nanoparticles were evaluated for particle size, zeta potential and morphology.The nanoparticle showed a good result, as the particle size was 53.25 nm and polydispersity index was 0.442, with the zeta potential value of + 31.5. The antibacterial activity result showed that the extract could inhibit the growth of Salmonella thypi, Staphylococcus aureus, and Bacillus subtilis in concentration of 500 ppm. However,the extract could not inhibit the growth of Escherichia coli in all tested concentrations. The nanoparticles showed an antibacterial activity against Staphylococcus aureus and Salmonella thypi in the concentrationof 250 up to 500 ppm whereasEscherichia coli and Bacillus subtilis in concentration of 500 ppm. The minimum inhibitory concentration (MIC) of the extract against Staphylococcus aureus and Bacillus subtilis were 400 ppm and againstSalmonella thypi in concentration of 500 ppm.In addition, the MIC of Escherichia coliwas more than 500 ppm. The MIC of the nanoparticles against Staphylococcus aureus was 300 ppm while against Bacillus subtilis and Salmonella thypi 400 ppm andagainst Escherichia coli500 ppm. Accordingly, the nanoparticles have a promising strategy to formulate the extracts to improve the antibacterial activity. Keywords: Antibacterial activity, ethanolic extract, Javanese turmeric, nanoparticles

    Time:
    10:25-10:50

    Coffee Break 10:50-11:05

    Title: Brusatol Nanoparticles to Facilitate Clinical Translation for Chemotherapy

    Simeon K Adesina
    Howard University, USA

    Biography
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    Biography

    Simeon K Adesina
    Howard University, USA

    Dr. Simeon K. Adesina completed his Ph.D. at the Department of Pharmaceutical Sciences, Howard University. He has published several articles in peer reviewed journals and several abstracts and conference proceedings. He has a patent and another patent submission. He joined Howard University as Assistant Professor in 2012 and is currently an Associate Professor in the Department of Pharmaceutical Sciences, College of Pharmacy at Howard University



    Abstract
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    Abstract

    Simeon K Adesina
    Howard University, USA

    Brusatol has been reported to possess a unique potential to target two distinct pathways that are important in the progression of cancers by its specific action on nuclear factor erythroid 2-related factor 2 (Nrf2) and as a global protein synthesis inhibitor. Due to its non-selective mechanisms of action, it is unlikely that brusatol can be administered as a therapeutic agent without substantial host toxicity. The oil-in-water emulsification solvent diffusion method was modified to prepare brusatol-loaded, polyethylene glycol-coated polylactide-co-glycolide nanoparticles. Scanning electron microscopy revealed the formation of nanoparticles and average size was determined by dynamic light scattering. Drug content of the nanoparticle formulation was determined by high performance liquid chromatography. The in vitro release isotherm showed a biphasic and sustained release of the encapsulated drug. Toxicity of the brusatol-loaded nanoparticles in prostate cancer cell lines was evaluated over 120 hours using the Cell Titer 96® Non-Radioactive Cell Proliferation Assay. In vitro cytotoxicity data revealed that both brusatol-loaded nanoparticles and the control brusatol solutions at the same concentrations exhibited dose-dependent toxicity to PC-3 and LNCaP prostate cancer cell lines at the concentrations used in this study with the nanoparticle formulation showing more toxicity to both cell lines. Cellular uptake of nanoparticles was evaluated using confocal microscopy after a 6-hour incubation of rhodamine-123-loaded nanoparticles with PC-3 cells. Data showed internalization of the nanoparticles. This study shows that the nanoparticle formulation of brusatol can overcome delivery challenges and facilitate the clinical translation of the compound for the treatment of cancers.

    Time:
    11:05-11:30

    Title: Microbiological and Cytological Study of the 1,3-Bis(Alkyl)-6-Methyluracils With 1,2,3– and 1,2,4- Triazolium Moieties Fragments

    A S Sapunova
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Biography
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    Biography

    A S Sapunova
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Anastasia Sapunova is a research assistant in the International research and innovation center of neurochemistry and pharmacology of A.E. Arbuzov Institute of Organic and Physical Chemistry. In 2016 she has had started her PhD in the Microbiology laboratory, A.E. Arbuzov Institute of Organic and Physical Chemistry Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of Russian Academy of Sciences", Kazan, Russia. Her main research topic is the development of new antimicrobial and anticancer drugs using new chemical compounds.”



    Abstract
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    Abstract

    A S Sapunova
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Currently, the drugs used in chemotherapy do not have a desired effect, due to their low selectivity and bioavailability, as well as high toxicity, which causes many side effects. It is known that compounds containing uracil fragment can exhibit biological activity through binding to different substrates. It is also found that the introduction of azole heterocycles leads to increased antimicrobial activity of compounds. Previously it was shown thatthe derivatives of 1,3-bis(alkyl)-6(5)-substituted uracil containing onium groups exhibit antimicrobial activity and low cytotoxicity in experiments on mammalian cells [1]. We have studied antimicrobial and anticancer activity, cytotoxicity and genotoxicity of new 1,3-bis(alkyl)-6-methyluracil with 1,2,3- and 1,2,4-triazolium moieties. The studied compounds have been tested for antimicrobial activity in relation to standard test strains of bacteria and fungi. Minimal Inhibitory Concentration of the leader compounds against Gram-positive bacteria is 0.4-4.0 mg/l and against Gram-negative bacteria - 8.0-31.3 mg/l. Antifungal activity of the investigated drugs in concentrations of 0.9-4.0 mg/l was shown. Then the mechanism of DNA damaging effect in the SOS-lux test was investigated. The given compounds do not have genotoxic properties and showed no DNA-damaging effect. The study of cytotoxic effect was carried out using the Cytell Cell Imaging system (GE Helthcare Life Science, Sweden). The compounds exhibiting high activity against tumor cell lines (MCF-7, A549, M-HeLa) and possessing low toxicity on normal human cell lines (WI-38 VA 13 subline 2RA, Chang liver) were identified. The results of this work show that the investigated compounds can be considered as potential antimicrobial agents and used as key components for new drugs. The work is financially supported by Russian Scientific Foundation (project No. 14-50-00014). References: 1 Voloshina A.D., Semenov V.E., Strobykina A.S., Kulik N.V., Krylova E.S., Zobov V.V., Reznik V.C. Synthesis, antimicrobial and toxicity properties of novel derivatives of 1,3-bis(alkyl)-6-methyluracil with 1,2,3- and 1,2,4-triazolium moieties // Bioorganic chemistry. -2017. –Vol.43. -№2. – P.197-204.

    Time:
    11:30-11:55

    Title: Antimicrobial Activity and Toxicity of New Macrocyclic and Acyclic Derivatives Of 1,3-Bis(Alkyl)-Chinazoline-2,4-Dione

    A D Voloshina
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Biography
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    Biography

    A D Voloshina
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    Alexandra Voloshina is a senior researcher with PhD degree in the International research and innovation center of neurochemistry and pharmacology of A.E. Arbuzov Institute of Organic and Physical Chemistry;the head of the microbiology laboratory in A.E. Arbuzov Institute of Organic and Physical Chemistry Subdivision of the Federal State Budgetary Institution of Science "Kazan Scientific Center of Russian Academy of Sciences", Kazan, Russia. Her professional interests focus microbiological and cytologic researches in the field of pharmacology and medicine.



    Abstract
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    Abstract

    A D Voloshina
    Kazan Scientific Center of Russian Academy of Sciences, Russia

    The urgency of the problem of new antimicrobial drugs development is determined by constant appearance of multiresistantmicroorganisms. Previously it was shown that 1,3-bis(alkyl)-6(5)-substituteduracilcontaining onium groups have a wide range of antimicrobial activity and low cytotoxicity in experiments on mammalian cells [1,2 ]. We have studied antimicrobial activity, mechanism of action, cytotoxicity and genotoxicity of new macrocyclic and acyclic derivatives of 1,3-bis(alkyl)-chinazoline-2,4-dione. The studied compounds have been tested for antimicrobial activity in relation to standard test strains of several pathogenic bacteria andfungi.The most active compoundswere investigated against resistant strains of Staphylococcus aureus.It has been found that the main structural factor influencing antimicrobial activity of compounds is atype of an alkyl radical in the onium group. It is shown that the macrocyclic and acyclic derivatives of 1,3-bis(alkyl)-chinazoline-2,4- dione have a high antibacterial activity against S. aureus strains resistant to ciprofloxacin and ammoxicillin. Antimicrobial activity against standard test strains of bacteria and fungi is manifested at the level of well-known drugs. The investigated compounds do not exhibit genotoxicity, show low cytotoxicity to mammalian cells andinhibite the glucose dehydrogenases activity of Staphylococcus aureus 209P and Candida albicans 855-653. Thus, a new class of biologically active compounds, which may be of significant interest for the development of new promising antimicrobial agents, was investigated. References: 1. V. E. Semenov, A.D.Voloshina, N.V. Kulik, A.S. Strobykina, R.Kh. Giniyatullin, L.F. Saifina, A.E. Nikolaev, E.S. Krylova, V.V. Zobov and V.S. Reznik. Russian Chemical Bulletin, International Edition. 2015, N.12, 2885—2896. 2. A.D.Voloshina, V.E.Semenov, A.S.Strobykina, N.V.Kulik, E.S.Krylova, V.V.Zobov, V.S.Reznik. Russian Journal of Bioorganic Chemistry.2017, 43, 170-176 The work is financial supported by Russian Scientific Foundation (project No. 14-50-00014).

    Time:
    11:55-12:20

    Title: Anti-Cancer Activity of the Phytochemical Indicaxanthin: in Vitro and in vivo Studies Against Melanoma

    A Attanzio
    University of Palermo, Italy

    Biography
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    Biography

    A Attanzio
    University of Palermo, Italy

    Alessandro Attanzio is a Researcher in- BIO/10 Biochemistry and completed his PhD in Pharmaceutical Sciences and got a Graduate degree in Biomedicine with a vote of 110/110 and praise. He is a Patent Inventor and Guest Editor for Journal of Food Quality. And is the Author of 25 publications on international biochemical and nutraceutical journals.



    Abstract
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    Abstract

    A Attanzio
    University of Palermo, Italy

    Epidemiologic studies show promising results supporting the role of natural compounds in the chemoprevention of melanoma. Indicaxanthin is a betalain pigment from cactus pear fruit, capable of modulating specific redox-driven pathways involved in the inflammatory reaction in vitro (1). Interestingly, indicaxanthin is bioavailable and exerts strong anti-inflammatory effects when orally administered at nutritionally-relevant doses in rats (2). A causative link between inflammation and melanoma has recently been explored. In line with this, we investigated the antitumor potential of indicaxanthin vs melanoma both in vitro and in in vivo mouse model. Indicaxanthin inhibited proliferation of human A375 melanoma cells. The inhibition measured at 24h was concentration-dependent in the range between 50 and 200 μM, with a maximum of 52% at the highest concentration. Indicaxanthin induced cell apoptosis as cytofluorimetrically revealed by double AnnexinV/PI staining. Moreover indicaxanthin time-dependently inhibited the activation of NF-kB, a transcriptional factor conferring tumor survival capacity and escape from apoptosis. In addition, the expression of Bcl-2 and c-FLIP, two inhibitors of apoptosis the expression of which is modulated by NF-kB, was decreased. More importantly, indicaxanthin (3.2 mg/kg) orally-administered for 15 days to mice when the injected tumor had reached an average 3-4 mm diameter, induced a reduction of tumor volume (86%) and weight (83%).

    Time:
    12:20-12:45

    Title: Evaluation of the Antimicrobial Effect of Expired Oral Antibiotics In-Vitro

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy, Saudi Arabia

    Biography
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    Biography

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy, Saudi Arabia

    Dr. Sasha Suliman holds an MSc degree in clinical pharmacy, from the University of Kabangsaan Malaysia (UKM) , As human beings experience a disease or trauma, her great passion is bringing healing to them. She finds herself in providing knowledge to others specially, for students to help sick people getting better. So keen in building a transparent relationship with her students’ because she believes that through them and she will be able to achieve her goal in healing people. There is no single approach is the right one for every individual, and got trained herfelf to deal with each individual.



    Abstract
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    Abstract

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy, Saudi Arabia

    Introduction: In spite of significant risks and the non-clinical importance due to loss of potency, stiff penalties against administration of expired medications are still not appropriately enforced by health policy makers in developing countries [1] Objective: To investigate the effect of expiration on in vitro potency of oral antibiotic Methodology: Azithromycin, Clarithromycin, levofloxacin, cloxacillin, Tetracycline, Doxycycline; both control [un expired] and investigated [expired] antibiotic were compered based on the antimicrobial potentials by challenging local isolates of different concentration of S.aureus using agar diffusion technique Results: All Azithromycin, Clarithomycin, Levofloxacin and Cloxacillin showed significant difference (P- values: 0.005, 0.000, 0.000 and 0.001 respectively). The Difference in means of zone inhibition were 4.3mm ,7.3 mm ,8.3mm and 5.29mm respectively, which is still within the accepted efficacy range

    Time:
    12:45-13:10

    Lunch Break: 13:10-14:10

    Title: Effects of Mesoglycan on Tissue Repair by Modulating Keratinocytes, Fibroblast and Endothelial Cells Functions

    Antonello Petrella
    University of Salerno, Italy

    Biography
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    Biography

    Antonello Petrella
    University of Salerno, Italy

    Antonello Petrella is a Professor of Pharmacology in the Department of Pharmacy at the University of Salerno, Italy. His professional interests involve the role of Annexin A1 in migration and invasion of human pancreatic carcinoma cells and in prostate cancer progression. Moreover studying the role of mesoglycan in skin wound healing. He is the Author, and has published about 60 scientific papers in refereed journals.



    Abstract
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    Abstract

    Antonello Petrella
    University of Salerno, Italy

    Glycosaminoglycans are polysaccharides of the extracellular matrix supporting skin wound closure. Mesoglycan is a mixture of glycosaminoglycans such as chondroitin-, dermatan-, heparan-sulfate and heparin. Glycosaminoglycans may contribute to the re-epithelialization in the skin wound healing, as components of the extracellular matrix. Here we describe, for the first time, the effects of mesoglycan in vitro cultures of adult epidermal keratinocytes and dermal fibroblasts. Once confirmed the lack of cytotoxicity by mesoglycan, we have shown the increase of S and G2 phases of fibroblasts cell cycle distribution. We further report the strong induction of cell migration rate and invasion capability on both cell lines, two key processes of wound repair. In support of these results, we found significant cytoskeletal reorganization, following the treatments with mesoglycan, as confirmed by the formation of F-actin stress fibers. Additionally, together with a significant reduction of E-cadherin, keratinocytes showed an increase of CD44 expression and the translocation of ezrin to the plasma membrane. Furthermore, as showed by immunofluorescence assay, fibroblasts treated with mesoglycan exhibited the increase of Fibroblast Activated Protein α and a remarkable change in shape and orientation, two common features of reactive stromal fibroblasts. Finally, we show the in vitro effects of mesoglycan in the new vessels formation by endothelial cells, since angiogenesis represents a key moment in wound healing. We found a strong increase of migration and invasion rates of these cells treated with mesoglycan, which mediate the activation of the pathway triggered by CD44 receptor. Interestingly, endothelial cells form longer capillary-like structures with a great number of branches, in the presence of the same treatments. Thus, the device, thanks to the mesoglycan, leads the cells to the Endothelial-to-Mesenchymal Transition, suggesting the switch to a fibroblast-like phenotype, as shown by immunofluorescence assays. In conclusion, based on these findings we suggest that mesoglycanmay be able to accelerate the healing process in venous skin ulcers, principally enhancing re-epithelialization granulation processes.

    Time:
    14:10-14:35

    Title: Overexpression of Ifnβ1 Gene in Mice Prevents Diet-Induced Obesity and Its Complications in Mice

    Mohammad Alsaggar
    Jordan University of Science and Technology, Jordan

    Biography
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    Biography

    Mohammad Alsaggar
    Jordan University of Science and Technology, Jordan

    Mohammad Alsaggar is an Assistant Professor of Pharmaceutical Biotechnology and Gene Therapy from Jordan. Mohammad received his BS in pharmacy in 2009 from the Jordan University of Science and Technology (Irbid, Jordan), and his PhD in Pharmaceutical and Biomedical Sciences from University of Georgia (Georgia, USA) in 2016. Mohammad’s research focusses on the use of gene therapy strategies for management of cancer and obesity diseases. In addition, Mohammad is working on cancer metastasis modeling using the hydrodynamic cell delivery technique for the assessment of tumor microenvironment impacts on the behavior of tumor cells growing in different organs.



    Abstract
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    Abstract

    Mohammad Alsaggar
    Jordan University of Science and Technology, Jordan

    The increased prevalence of obesity is raising global concerns, largely because of associated complications of the disease. In spite of significant research in the field, few therapies have been approved, and therapeutic outcomes remain insufficient. Therefore, alternative therapies are in need. Gene therapy has emerged as powerful strategy to tackle many of human diseases by modulation of gene expression levels. In this work, murine interferon beta 1 (IFNβ1)gene was overexpression using hydrodynamic gene delivery method in mice to prevent development of obesity. Using diet induced obesity model in mice, we showed that IFNβ1 suppressed inflammation in adipose tissue adipose tissue hypertrophy. Also, it modulated cytokines expression toward anti-inflammatory interleukins. Importantly, IFNβ1 overexpression blocked weight gain without impacting food intake, and restored glucose homeostasis in treated mice. Together, these data suggest that IFNβ1 is a powerful anti-inflammatory cytokine with promising potential to treat inflammatory disorders. In addition, gene therapy is promising strategy to treat obesity and its related complications.

    Sessions:
    Pharmaceutical Management & Pharmacognosy & Phytochemistry & Pharmaceutical Nanotechnologies

    Time:
    14:35-15:00

    Title: Attitudes and Opinion of Hospital Pharmacists towards Extemporaneous Compounding and Related Issues in Khartoum City: Part II

    Shayoub M EL
    Khartoum University, Sudan

    Biography
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    Biography

    Shayoub M EL
    Khartoum University, Sudan



    Abstract
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    Abstract

    Shayoub M EL
    Khartoum University, Sudan

    Objectives: To examine the frequency, nature and extent of extemporaneous compounding undertaken by Sudanese hospital pharmacists and determine their attitude on aspects related to extemporaneous compounding. Methods: 200 questionnaires were administered to randomly selected hospital pharmacies in Khartoum city. Feedback to most question was measures on 1-5 Likert scales. Data was collected from December 2015 to March 2016. Results: Response rate was 60%; 75.2% were females and 24.8% were males. 28.2% of respondents compounded 1-5 prescriptions weekly that comprised 3-5% of total prescriptions; most prescriptions were dermatological preparations; major reasons for compounding were product (53%), pediatric strength 42% and official preparations 41%; Major prescribers were dermatologist; Pharmacist who often compound prescriptions; appreciable number of respondents rated standard of equipments and quality of ingredients as high, respectively; 25% and 15.8% of respondents indicated that lack of ingredients and lack of time limited their immediate extemporaneous dispensing; Factors that greatly inhibit compounding were lack of equipments, lack of practice in compounding and time constrains; 49.6% of respondents stated that demand for compounding greatly decreased over the pat few years; 52.2% of the respondents thought that, extemporaneous compounding will be decreased in the future; 54.0% of respondents felt that their skill in compounding were increased since graduation; most respondents had high confidence and professional satisfaction in extemporaneous compounding; 54% of respondents felt that their training was inadequate and emphasis should be placed in training 31,7%; 71.2% agreed for periodical assessment of pharmacists in compounding; overall respondents felt that a refreshing course in extemporaneous compounding is useful. Conclusion Extemporaneous compounding constitutes small part of hospital pharmacy activities, and respondents were strongly agreed that it should be kept within the profession. Several areas of improvement were identified and continuous education was highly demanded.

    Time:
    15:00-15:25

    Title: The Effect of Nutrition Program On Health Status Among Dialysis Patients

    Mazen El-Sakka
    Al Azhar University-Gaza, Palestine

    Biography
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    Biography

    Mazen El-Sakka
    Al Azhar University-Gaza, Palestine



    Abstract
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    Abstract

    Mazen El-Sakka
    Al Azhar University-Gaza, Palestine

    Poor access to food among low-income adults has been recognized as a risk factor for chronic kidney disease (CKD), but there are no data for the impact of food insecurity on progression to end-stage renal disease. Diet and anthropometrics measurements are associated with increased morbidity and mortality and a rapid deterioration of kidney function in patients with chronic kidney disease. However, there is little information regarding the effect of nutrition intervention. The aims of this study were to investigate whether of diet patterns and changes in biochemical parameters as well as eGFR among adults with CKD on dialysis, as well as,to evaluate the efficacy and safety of a nutrition education program in patients with dialysis, based on the diagnostic criteria for Protein–energy wasting. The design of the study was a 2-month Control experimental design, prospective, and interventional study. The study was started from May2017and expected to finish onDecember 2011 in the Dialysis Department of Al Shifa Hospital in Gaza, Palestine. Subjects A total of 133 patients with ESRD started the research study, 102 finished it and 51 subjected on very well controlled diet program. Intervention The 2-month nutrition education program consisted of designing an individualized diet plan based on the patient's initial nutritional status, and 4 nutrition education sessions. Results and Conclusion Promising results reveal the effectof diet program on anthropometric, biochemical, andeGFR. Further research is needed to investigate the reliability and utility of this tool in a larger population group.

    Time:

    coffee Break: 15:50-16:05

    Title: Anti-Alopecia Activity of Hantap (Serculia coccinea Jack.) Leaves Ethanol Extract

    Resmi Mustarichie
    Padjadjaran University, Indonesia

    Biography
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    Biography

    Resmi Mustarichie
    Padjadjaran University, Indonesia

    Resmi Mustarichie is Professor of Pharmaceutical Chemistry . He was graduated as PhD. Graduate supervised by Prof. Allan F.M Barton from Murdoch University, Western Australia. He is currently active as lecturer and researcher at Faculty of Pharmacy, UniversitasPadjadjaran, Indonesia, 45363.His interest in Analysis and determination of bioactive compounds from natural materials, Herbal anti-alopecia, and molecular computing



    Abstract
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    Abstract

    Resmi Mustarichie
    Padjadjaran University, Indonesia

    Objective: This study aims to determine and verify the use of ethanol extract of Sterculiacoccinealeaves ethanol extract as stimulant of hair growth or anti-alopecia. Methods: The S. coccinealeaves was collected from Salawu tribe, Tasikmalaya, West Java. The extraction was done by maceration which was based on standard method of Indonesian Herbal Pharmacopeae. The viscous concentrated extract was fractionated by liquid-liquid extraction. Phytochemical screening according to the Farnsworth method. Method of hair growth on Angora type rabbit was modified of Tanaka method. Results: The results of phytochemical screening using Farnsworth method showed the ethanol extract containing secondary metabolite of tannin compound, polyphenol, steroid, triterpenoid, quinone, monoterpenoid and sesquiterpenoid. The results of hair fertilizer testing using Tanaka method showed that ethanol extract and water extract with 20%, 15%, 10% concentration significantly could fertilize hair with test for 18 days. Extracts of ethanol with levels greater than 10% showed better results. Water fraction of 10% appeared to show the best result for rabbit hair growth overcome minoxidil. Conclusions: This work found that the concentration of 10% ethanol extract of S. coccinea and its water fractions were affective for hair growth on male rabbits. It was suggested, however, for further study to determine the compound which was responsible by using elucidation methods and being tested to volunteers. Keywords: Hair fertilization, ethanol extract, Sterculiacoccinea, hair grower, Alopecia, phytochemical screening

    Sessions:
    Poster Presentations

    Time:

    Title: Impact Of Subcutaneous Trastuzumab On the Uptake Of Intravenous Trastuzumab In UK

    Mohammed Aladul
    Keele University, UK

    Biography
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    Biography

    Mohammed Aladul
    Keele University, UK

    I qualified as a pharmacist in 2007 after graduating from University of Mosul, Iraq and preregistration training at Avicenna General Hospital in Mosul. I then worked as a hospital pharmacist and studied for an MSc in Clinical Pharmacy, graduating in 2013. Subsequently I moved to the University of Mosul as Director of Pharmacy. I started my PhD at the School of Pharmacy Keele University, in 2015, undertaking both quantitative and qualitative analysis of biosimilar, have published 5 research articles to date based on this work.



    Abstract
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    Abstract

    Mohammed Aladul
    Keele University, UK

    Trastuzumab is indicated for the treatment of early stage breast cancer which over expresses human epidermal growth factor receptor 2 (HER-2), metastatic breast cancer and metastatic gastric cancer with HER-2 positive tumours. A subcutaneous (SC) formulation for trastuzumab has been marketed in UK since September 2013. Studies showed that SC trastuzumab are more cost effective and more preferred by healthcare professionals and patients than intravenous (IV) trastuzumab. Biosimilar trastuzumab launch is anticipated in the UK market in 2018 in IV dosage form. Purpose To investigate the impact of the introduction of SC trastuzumab on the utilisation of intravenous trastuzumab and size of accessible market for biosimilar trastuzumab in the UK. Methods An autoregressive integrated moving average model was conducted on the secondary care utilisation data of trastuzumab (in grams) from the DEFINE database, between 2012-2017. Results The overall utilisation of trastuzumab doubled following the launch of SC trastuzumab from 2831g in January 2012 to 5697g in December 2017. The utilisation of IV trastuzumab decreased significantly following the introduction of SC trastuzumab. The utilisation trend of the IV formulation before and after the introduction of SC trastuzumab were 28.3 and -57.8 g/month (p>0.004) respectively. The utilisation trend of SC trastuzumab increased significantly by 58.1 g/month (p>0.001) and achieved 74% of the trastuzumab market by December 2017. Conclusion SC trastuzumab changed dramatically the market profile of trastuzumab and reduces the accessible IV market share for competition with upcoming biosimilar trastuzumab. Previous research has shown that biosimilars launched in recent years have entered the market at 30% less than the originator brand and prices have stabilised at approximately 50%, but these have all been with similar formulations. It is not yet clear whether such price decreases will be enough to change the current market profile of trastuzumab in the UK.

    Time:

    Title: Inhibition of Nuclear Factor- B Reduces Brain Inflammation in Rats

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Biography
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    Biography

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Prof. Kaplanski completed his Ph.D. in pharmacology in the Department of Pharmacology in the Hebrew University in Jerusalem (Israel). Thereafter, he completed his post-doctoral fellowship in the Department of Pharmacology in the Free University of Amsterdam (The Netherland). He mentored numerous M.Sc. and Ph.D. students in the fields of Pharmacology and exercise physiology. He has authored more than 100 peer-reviewed research papers.



    Abstract
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    Abstract

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Introduction. A large body of evidence suggests that inflammation is involved in the pathophysiology of several neurological disorders, including psychiatric illnesses, neurodegenerative diseases and stroke. Nuclear Factor- B (NF-B) is a cellular pathway that plays a prominent role in numerous immune and inflammatory responses in mammals. Aims. This study was undertaken to examine the effects of a selective NF-B inhibitor, JSH-23, on lipopolysaccharide (LPS)-induced inflammation in rats. Materials and Methods. Rats were treated with JSH-23 (10 mg/kg) through a single intraperitoneal (ip) injection. JSH-23 was administered at (i) 2.5 h before or at (ii) 1.5 h after LPS (1 mg/kg, ip) injection. At 4 h (i) and 2.5 h (ii) after JSH-23 administration, rats were sacrificed, blood was collected and different brain regions were excised. Levels of the inflammatory constituents interleukin (IL)-6, prostaglandin (PG) E2, tumor necrosis factor (TNF)- and nuclear phosphorylated p65 (P-p65) in plasma and brain were examined by specific ELISA kits. Results. The effects of JSH-23 on plasma and brain inflammatory mediators' levels differed between the pre- and - post-LPS administration schedule and between the various brain regions. Mostly, JSH-23 treatment reversed the changes in IL-6, PGE2, TNF- and P-p65 levels in plasma, frontal cortex, hippocampus and hypothalamus of LPS-treated rats. Conclusions. These results suggest that inhibition of NF-B may have a therapeutic potential for the treatment of inflammation-associated brain disorders.

    Time:

    Title: Anthocyanidins and Anti-Obesity Drug Liraglutide Promote Human Adipose Mesenchymal Cell Differentiation into Chondrocyte and Osteocyte Lineages

    Liga Saulite
    University of Latvia,Latvia

    Biography
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    Biography

    Liga Saulite
    University of Latvia,Latvia

    Liga Saulite is a PhD student at Pharmacy study program of Faculty of Medicine of University of Latvia. She has received BSc and MSc degrees in Biology at University of Latvia. She has studied Biochemistry and Molecular Biology at University of Bremen and Molecular Cancer biology and laboratory animal welfare in University of Oslo. Her main expertise includes mesenchymal stem cells, breast cancer cells, nanoparticles, neurodifferentiation and Schwann cells. She is co-author in four scientific publications.



    Abstract
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    Abstract

    Liga Saulite
    University of Latvia,Latvia

    Adipose tissue derived mesenchymal stem cells (aMSCs) are an attractive source for regenerative therapy due to their unique ability to form osteocytes, chondrocytes and adipocytes in vitro after appropriate stimuli. Therefore, aMSCs may serve as a valuable tool for screening of pharmacologically active compound effect on tissue engineering. Plant pigments anthocyanidins are known for their anti-oxidative, anti-inflammatory and anti-tumor properties as well as for influence on wound healing. Anthocyanidin effect on aMSC differentiation may lead to design of new therapeutic strategies for regenerative medicine. The objective of the current study was to evaluate the effect of anthocyanidins malvidin, cyanidin, delphinidin on the adipogenic, osteogenic and chondrogenic differentiation of human aMSCs. aMSCs (ATCC, PCS-500-011) were differentiated into adipocytes, osteocytes and chondrocytes by Gibco®StemPro® differentiation kits according to manufacturer’s instructions. 25µM malvidin, cyanidin and delphinidin (all from Sigma Aldrich) were added to aMSCs during the differentiation. The expression of adipogenesis, osteogenenesis and chondrogenesis related markers was analyzed by qPCR and ELISA. 10 and 100nM liraglutide (TRC Research) was used to compare anthocyanidin effects on aMSCs differentiation. Delphinidin decreased the expression of FABP4 and Adiponectin similarly to liraglutide in newly formed adipocytes. Malvidin and liraglutide increased the accumulation of calcium deposits and expression of Runx2 and BMP-2 in the osteocytes. Delphinidin, cyanidin and liraglutide significantly induced the expression of Col2a1 and Aggrecan in the chondrocytes. Delphinidin and anti-obesity drug liraglutide inhibit formation of adipocytes from aMSCs. Malvidin and liraglutide induce the osteogenesis, whereas cyanidin, delphinidin and liraglutide promote osteogenic and chondrogenic differentiation. Acknowledgements/Funding. This study was supported by the European Regional Development Fund within the project No.1.1.1.1/16/A/047.

    Time:

    Title: Identification of Metabolite for mTOR Inhibitor, AZD8055 in Rats After a Single Oral Administration Using Ultra-Performance Liquid Chromatography and Mass Spectrometry

    Byung Hwa Jung
    Korea Institute of Science and Technology, Republic of Korea

    Biography
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    Biography

    Byung Hwa Jung
    Korea Institute of Science and Technology, Republic of Korea

    Byung Hwa Jung has her expertise in metabolomics and DMPK (drug metabolism and pharmacokinetics). She has developed analytical methods for the quantitative and qualitative determination of endogenous metabolites and xenobiotics (drugs). The platforms for the non-targeted metabolomics and lipidomics using LC-MS for the evaluation of metabolic change in in vivo and in vitro systems were already established in her lab. Along with these systems, the quantitative analytical platforms for more than 170 metabolites and several drugs were also set up. The mechanisms and biomarkers for disease generation and development, and drug effect and adverse effects are studied with those systems.



    Abstract
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    Abstract

    Byung Hwa Jung
    Korea Institute of Science and Technology, Republic of Korea

    Metabolite identification of AZD8055, which is an ATP-competitive specific dual mTOR inhibitorand exhibited potent antitumor activity on several types of solid tumors, was performed using ultra high-performance liquid chromatography-ion trap mass spectrometry (UHPLC-IT-MS) through both in vitro and in vivo approaches using rat liver microsomes (RLMs) and rat plasma, urine and feces, respectively. A total of eight putative metabolites (five phase I and three phase II) were identified, and a tentative metabolic pathway was suggested for the first time. Considering the accurate mass and mass fragmentations of the detected metabolites, their plausible structures were suggested. Demethylation, hydroxylation, oxidation and morpholine ring opening were the major biotransformation processes for the phase-I metabolism, while phase-II metabolites were merely generated by the glucuronide conjugation reaction. The cumulative excretion of AZD8055 in urine and feces was 0.13% and 1.11% of the dose, respectively. When the semi-quantitative analysis of the metabolites was performed using UHPLC-MS/MS (ultra-performance liquid chromatography tandem mass spectrometry) to evaluate the overall trend of metabolites formation and excretion, AZD8055 was excreted more in the form of the metabolites than itself and their formation was very fast. Therefore it was presumed that biotransformation was playing a crucial role in its elimination. Ultimately, this study provides novel insights regarding the in vitro and in vivo biotransformations of AZD8055. Further investigations of metabolites of this potent anti-cancer compound could be beneficial for the antitumor drug design and development process

    Time:

    Title: Inhibitory Effect of Mangiferin on Tyrosinase

    Renata Ochocka
    Medical University of Gdańsk,Poland

    Biography
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    Biography

    Renata Ochocka
    Medical University of Gdańsk,Poland

    Renata Ochocka is Professor of Pharmacy. She is the head of the Department of Biology and Pharmaceutical Botany at Faculty of Pharmacy, Medical University of Gdańsk, Poland. Her professional interests focus determination of bioactive compounds from medicinal plants and the investigation their biological activity.



    Abstract
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    Abstract

    Renata Ochocka
    Medical University of Gdańsk,Poland

    Mangiferin (2-C--glucopyranosyl-1,3,6,7-tetrahydroxyxanthone) is the polyphenol with strong antioxidant properties.Mangiferin is obtained from mango tree (Mangiferaindica, L. Anacardiaceae). Significant amount of mangiferin is observed in honeybush (Cyclopiasp., Fabaceace)and Anemarrhenaasphodeloides(Liliaceae ).It was proved that mangiferin exhibit many pharmacological activities. In our previous study we identified that mangiferin enhances apoptotic effects of hesperidin in human cervix adenocarcinoma cell line (HeLa) [1] and thatmangiferinhas the ability to penetratethrough the stratumcorneumbarrier and to the living skin layers, wherecollagenase and elastaseoccur [2]. We indicatedthat, mangiferininhibitselastase and collagenaseactivity in a reversiblemanner, referred to as a non-competitiveinhibition [2]. In the presentstudy, inhibitory effect of mangiferin on mushroomtyrosinaseactivitywereexamined.Tyrosinaseis a coppercontainingenzymewhichisresponsible for the first two steps of melanogenesis, process of melanin synthesis.Tyrosinasecatalyses melanin biosynthesis in human skin and epidermalhyperpigmentationmaycausevariousdermatologicaldisorders. The research proved that mangiferin inhibits tyrosinase activity in a dose dependent manner. Mechanism of inhibition was described as mixed inhibition with predominance of non-competitive inhibition.

    Time:

    Title: Anti-neuroinflammatory Effects of 12-Dehydrogingerdione in LPS-Activated Microglia Through Inhibiting Akt/IKK /NF-κB Pathway and Activating Nrf-2/HO-1 Pathway

    Hyun Ok Yang
    Korea Institute of Science and Technology,Republic of Korea

    Biography
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    Biography

    Hyun Ok Yang
    Korea Institute of Science and Technology,Republic of Korea

    Hyun Ok Yang has complete her PhD on pharmacognogy at Seoul National University in 1993. After Post-doctoral study at University of Iowa, USA, she is now a principal research scientist at Natural Products Research Center in Korea Institute of Science and Technology (KIST) & a professor of UST, KIST School, Republic of Korea



    Abstract
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    Abstract

    Hyun Ok Yang
    Korea Institute of Science and Technology,Republic of Korea

    Ginger, one of worldwide consumed dietary spice, however, it is not only famous as food supplements, and also believed to exert a variety of remarkable pharmacological activity as herbal remedies. In this study, a ginger constituent, 12-dehydrogingerdione (DHGD) was proven that has anti-inflammatory effect with comparable activity to positive control 6-shogaol in lipopolysaccharide (LPS)-activated microglial cells, including interleukin (IL)-6, tumor necrosis factor (TNF)-α,prostaglandin (PG) E2, nitric oxide (NO), inducible NO synthase (iNOS) and cyclooxygenase (COX)-2, without interfering with COX-1. Subsequent mechanistic studies indicate that 12-DHGD may inhibit neuro-inflammation through suppressing the LPS-activated Akt/IKK/NF-κB pathway. Furthermore, 12-DHGD markedly promoted the activation of NF-E2-related factor (Nrf)-2 and heme oxygenase (HO)-1, and we demonstrated that the involvement of HO-1 on pro-inflammatory mediators such as NO and TNF-α by using HO-1 inhibitor. These results indicate that 12-DHGD protects against neuro-inflammation by inhibiting Akt/IKK/IκB/NF-κB pathway and promoting Nrf-2/HO-1 pathway.

    Time:

    Title: :Vasodilator Effect in Human Internal Mammary Artery of Propofol: The Role of Potassium Channels

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Biography
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    Biography

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Dr Arslan is the head of pharmacology department in the Ankara Yildirim Beyazit University. His research interests focus on the experimental pathophysiology and inflammation of pulmonary and cardiovascular systems. He is the PhD supervisor for pharmacology and toxicology students.



    Abstract
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    Abstract

    Seyfullah Oktay Arslan
    Ankara Yildirim Beyazit University, Turkey

    Propofol is an intravenous anesthetic that can be used for the induction and maintaining of anesthesia. The purpose of this study is to investigate the mechanism of the vasodilator action of propofol in the human internal mammary artery (IMA). The IMA rings were hung in isolated organ baths and the changes in tension were recorded isometrically. Potassium chloride (KCl) and phenylephrine (PE) were added to organ baths to form precontraction. Propofol (1, 10, 100 μM) was added cumulatively when the precontractions were stable. The antagonistic effect of propofol on KCl (mM), PE (1 µM), 5-hydroxytryptamine (5-HT, 30 µM) and calcium chloride (CaCl2, 10 μM-10 mM)-induced contractions in the vascular rings were investigated. Propofol-induced relaxations were also tested in the presence of the large conductance calcium-activated potassium channel inhibitor tetraethylammonium (TEA, 1 mM), the adenosine triphosphate-sensitive potassium channel inhibitor glibenclamide (GLY, 10 µM), the voltage-sensitive potassium channel inhibitor 4-aminopyridine (4-AP, 1 mM) and the inward rectifier potassium channel inhibitor barium chloride (BaCl2, 30 µM). Preincubation with propofol (1, 10, 100 μM) did not affect the basal tone but inhibited the KCl, PE, 5-HT and CaCl2-induced contractions. Propofol-induced relaxation was not affected by 4-AP, GLI, BaCl2. But, TEA inhibited propofol-induced relaxations significantly. Present experiments show that propofol relaxes contracted IMA and inhibits the KCl, FE, 5-HT and CaCl2-induced contractions. The results demonstrate that the mechanism of action of propofol-induced vasodilation in the IMA may be related to BKCa activation. Key Words: Propofol, Internal Mammary Artery, Potassium Channels, Vasodilation

    Time:

    Title: Vasodilator Effect of Propofol in Rat Aorta

    Seyfullah OktayArslan
    Ankara Yildirim Beyazit University, Turkey

    Biography
    χ

    Biography

    Seyfullah OktayArslan
    Ankara Yildirim Beyazit University, Turkey

    Dr Arslan is the head of pharmacology department in the Ankara Yildirim Beyazit University. His research interests focus on the experimental pathophysiology and inflammation of pulmonary and cardiovascular systems. He is the PhD supervisor for pharmacology and toxicology students.



    Abstract
    χ

    Abstract

    Seyfullah OktayArslan
    Ankara Yildirim Beyazit University, Turkey

    Propofol is an intravenous anesthetic that can be used for the induction and maintaining of anesthesia. In this study, it was aimed to investigate the mechanism of vasodilator action of propofol in rat aorta (RA). The RA rings were suspended in isolated organ bath and tension was recorded isometrically. First, potassium chloride (KCl) and phenylephrine (PE) were added to organ baths to form precontraction. When the precontractions were stable, propofol (1, 10, 100 μM) was added cumulatively to the baths. The antagonistic effect of propofol on KCl (mM), PE (1 µM), 5-hydroxytryptamine (5-HT, 30 µM) and calcium chloride (CaCl2, 10 μM-10 mM)-induced contractions in the vascular rings were investigated. Propofol-induced relaxations were also tested in the presence of the large conductance calcium-activated potassium channel inhibitor tetraethylammonium (TEA, 1 mM), the adenosine triphosphate-sensitive potassium channel inhibitor glibenclamide (GLY, 10 µM), the voltage-sensitive potassium channel inhibitor 4-aminopyridine (4-AP, 1 mM) and the inward rectifier potassium channel inhibitor barium chloride (BaCl2, 30 µM). Preincubation with propofol (1, 10, 100 μM) did not affect the basal tone but inhibited the KCl, PE, 5-HT and CaCl2-induced contractions. Propofol-induced relaxation was not affected by 4-AP, GLI, BaCl2. But, TEA inhibited propofol-induced relaxations significantly. Comparison among multiple groups was made by using a one-way ANOVA followed by Scheffe's post hoc test to determine significant differences among the means of the data groups. The propofol induces relaxation in contracted RA and inhibits KCl, FE, 5-HT and CaCl2-induced contractions. The results demonstrate that the mechanism of action of propofol-induced vasodilation in the RA may be related to BKCa activation. Key Words: Propofol, Rat Aorta, Potassium Channels, Vasodilation

    Time:

    Title: Small Molecule Discovery for the Treatment of Alzheimer's Disease by Virtual Screening and Pharmacological Evaluation

    Nerlis Pajaro Castro
    University of Sucre, USA.

    Biography
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    Biography

    Nerlis Pajaro Castro
    University of Sucre, USA.

    I am a pharmaceutical chemist, with a masters degree in pharmaceutical sciences and PhD candidate in environmental toxicology. At the present time I am associate professor at the faculty of health sciences of the University of Sucre. My work in research is focused on the area of famacology, toxicology, in silico drug design and chemistry medicine. Therefore, since several years I have been working on several research projects related to discovering molecules for the treatment of Alzheimer through in silico search and pharmacological evaluation, and evaluation of the toxicity of organic compounds using the insect Tribolium castaneum as in vivo model.



    Abstract
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    Abstract

    Nerlis Pajaro Castro
    University of Sucre, USA.

    Current treatments for Alzheimer's disease are focused on symptomatic threrapy, since not targets have been identified. The aim of this study was to discover small molecules acting on β-Secretase, γ-Secretase, Pin1 and Cdk5 proteins, and its pharmacological evaluation. The proteins 3D structures were downloaded from the PDB database and were docked, using AutoDock Vina, with molecules having drug-like properties. Initial analysis results reveal four potential compounds capable of binding to evaluated proteins which have affinity values ranging between -6.8 and -9.1 Kcal/mol. The interaction of ligands 84554447, 84577234, 84577855 and 84378305 with 4U84 protein (PDB ID), allowed the identification of residues SER32, ALA31 and LYS97 as the main amino acids involved in the protein-ligand docking. In the case of the 4UPC protein, the residues identified were CYS230, VAL51, ALA172, PHE287, ARG285, TYR173 and THR227. With 3VF3 protein, the amino acids were GLN73, THR218 and GLY217 and with 3O0G, the residues were ALA31, ASN144 and VAL64. Of the compounds having the highest affinity value, one was selected to perform the pharmacological evaluation. Thus, the compound 84378305 (Pubchem ID) was synthesized by chemical synthesis, to subsequently evaluate its neuroprotective effect, by the MTT and DHL release assays. The pharmacological evaluation showed that the synthesized compound has low cytotoxicity and is an effective neuroprotect. In conclusion, it was possible to find by in silico and experimental evaluation a ligand capable of acting against proteins related to Alzheimer's disease and which possess neuroprotective activity.

    Time:

    Title: Substance-Use Attitudes, Behaviors, Education and Prevention in Colleges of Pharmacy

    Samah AL-Shatnawi
    Jordan University of Science and Technology, Jordan

    Biography
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    Biography

    Samah AL-Shatnawi
    Jordan University of Science and Technology, Jordan

    Samah AL-Shatnawi Ph.D, is an assistant professor at the Clinical Pharmacy department, Jordan University of Science and Technology. Her research interests span over several areas related administrative pharmacy fields, including pharmaceutical behaviors, behavioral substance use, substance use disorders, and health outcomes. Within Pharmacy Practice, substance-use behaviors and disorders among healthcare professionals (pharmacists and student pharmacists) is one of her favorite research topics. Samah AL-Shatnawi earned Pharm.D degree from Jordan University of Science and Technology (JUST), Jordan in 2009. Immediately after graduation, she worked as a researcher and teaching assistant at JUST and she was awarded JUST Clinical Pharmacy Research Scholarship (2012-2015) for pursuing her Ph.D. degree. For Ph.D. dissertation, she applied theoretical models and utilized quantitative research methods to study Alcohol Use Behaviors and Outcomes in Professional Student Pharmacists. During her graduation study period, she co-authored articles and several presentations as a primary and secondary author. Currently she is supervising many master-degree students to conduct prospective (primary data) and retrospective (secondary data) research within the field of pharmacy practice.



    Abstract
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    Abstract

    Samah AL-Shatnawi
    Jordan University of Science and Technology, Jordan

    Among healthcare professionals, substance-use behaviors and disorders greatly affect the provision of healthcare services (e.g. pharmaceutical services). Pharmacists play a central role in medical care and are medication experts, however, they are considered as highly vulnerable to substance-use disorders. Thus, pharmacy practice and patients’ health might be threatened. As compared to general college students, research suggests that healthcare professional students are at higher risk for problematic substance-use behaviors. However, less research has examined substance-use among student pharmacists in comparison to other healthcare professional students. This study represents a literature review that describes and summarizes student pharmacists’ substance-use behavior in the United States. The purposes of literature review were: to highlight what is known about substance-use behaviors among student pharmacists, and to identify factors that might influence problematic substance-use behaviors among them. This review includes studies completed within US colleges and universities identified through multiple databases. Articles on student pharmacists’ substance-use behaviors focusing on substance-use rates or levels, motives for any substance-use, and substance-use related problems were included. The literature search identified 16 studies. Current literature indicates that there are problems with alcohol and other drug use among student pharmacists. Although researchers have found variations in the type and rate of reported substance-use, significant proportions of student pharmacists were identified as being at high risk for substance-use disorders. Findings suggest that pharmacy schools should encourage and stimulate more research in order to implement effective screening and early intervention programs in an effort to address this important student health issue.

    Time:

    Title: Biochemical and Physicochemical Study of a Virgin Oil of Pistacia

    Merzougui Imene
    University Mouhamed kheidar Biskra, Algeria

    Biography
    χ

    Biography

    Merzougui Imene
    University Mouhamed kheidar Biskra, Algeria



    Abstract
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    Abstract

    Merzougui Imene
    University Mouhamed kheidar Biskra, Algeria

    This study has allowed to confirm the physicochemical characteristics and fatty acid composition by GC of the oil of Pistacia lentiscus extracted by traditional method and evaluate its effect on some blood lipid parameters. The results showed that the main physicochemical characteristics of Pistacia lentiscus oil are: moisture (0.84%), a relatively high iodine value (80,44) indicating that this oil has an important degree of unsaturation. The oil is mainly composed of unsaturated fatty acids (MUFA) where oleic acid dominate with 47,01% of total fatty acids and PUFAs represented by linoleic acid (19,26%). Concerning the biological survey, oil, at 10% and 20% doses of diet for 15 and 30 days of two periods of treatment, resulted in beneficial effects on the lipid profile of Wistar albinos rats previously fed with animal and vegetable fats .We observed decreases in total cholesterol, triglycerides (TGA), total lipids and LDL-C, and an increase in HDL-C "good cholesterol" probably related to the presence of a large amount of (MUFA) and (PUFA). Keywords: Pistacia Lentiscus, oil, lipid profile, monounsaturated fatty acids, polyunsaturated fatty acids

    Time:

    Title: Ivstigation of the Susceptibility of Standard Biofilm Forming Bactrial Isolates of Methicillin Resistantstaphylococcusaureus (Mrsa) To Selected Antibiotics from the Fluoroquinolone Family, an In-Vitro Study

    Majed M. Masadeh
    Jordan University of Science and Technology, Jordan

    Biography
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    Biography

    Majed M. Masadeh
    Jordan University of Science and Technology, Jordan

    Dr.MajedMasadeh is an Associate Professor of Pharmaceutical Microbiology at the Pharmacy College of Jordan University of Science and Technology, where he teaches pharmaceutical microbiology and biotechnology, pharmacology and basic microbiology courses (since Sep 2005). Dr.Majed holds a Doctor of Philosophy (Ph.D) degree in Pharmacy (majoring in Pharmaceutical Microbiology) from The University of Abertay Dundee at United Kingdom (UK). In addition, Dr.Masadeh holds a Bachelor of Science degree in Microbiology (India), Master of Science in Medical Microbiology (India). Dr.Majed has expertise in bacterialbiofilm resistance investigation towards antimicrobial agents, research design, and scientific writing. Dr.Majed has published 38 papers in peer reviewed journals including the Current Microbiology, Drug Design, Development and Therapy, Annals of Clinical Microbiology and Antimicrobials , Infection and Drug Resistance, International Journal of Integrative Biology, Clinical Medicine Research, Pharmaceutical Biology, Cytotechnology, International Journal of Occupational Medicine and Environmental Health,Journal of Infection in Developing Countries,Electromagnetic Biology and Medicine, Prevalence of Depression among Relatives of Cancer Patient in Jordan .Palliative & Supportive Care, Journal of Pharmacy and Bioallied Sciences, Pakistan Journal of Pharmaceutical Sciences, etc...In Sep 2013, Dr.Majed wasselected inthe Academickeys Who's Who in Pharmacy Higher Education (WWPHE) from September 2013. Dr. Majedreviewer for: International Journal of Nanomedicine: Dove Medical Press,International Journal of Medical Devices: Evidence and Research,British Journal of Pharmaceutical Research (BJPR), European Journal of Medicinal Plants,British Microbiology Research Journal,International Journal of Cell Culture and Biotechnology, (Cytotechnology), JJBS for Jordan Journal of Biological Sciences etc...



    Abstract
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    Abstract

    Majed M. Masadeh
    Jordan University of Science and Technology, Jordan

    Bacteria have the ability to form complex surface-associated communities known as biofilms. These biofilms are commonly associated with many health problems, as many persistent and chronic bacterial infections are thought to be linked to biofilm formation. Compared to planktonic cells, biofilms are characterized by significant antibiotic resistance as well as high virulence potential, which explain why biofilms are associated with tremendous impact on health including increased morbidity and mortality. Moreover, complications related to biofilms often result in additional hospitalization and medical care for patients, leading to substantial economic impact.In this study we are proposing to investigate in vitro susceptibility of methicillin resistant Staphylococcusaureus (MRSA) strain ATCC 43300 to selected antibiotics from the fluoroquinolone class. Antibiotics efficacy against MRSA biofilm bacteria is compared to planktonic state. The susceptibility of the six fluoroquinolones demonstrated variable activity against MRSAbacteria as they were not equipotent. For planktonic cells, the MIC values showed that moxifloxacin (0.049µg/ml), Gatifloxacin (0.078µg/ml) and levofloxacin (0.156µg/ml) were the most effective, while norfloxacin (1.172µg/ml) was the least effective. This was confirmed by measuring the diameter of the zone of inhibition. The results showed the same order of activity as the MIC values against planktonic cells. The biofilm cells, on the other hand, demonstrated less sensitivity against toward the same antibiotics used against planktonic counterparts. In this case, Gatifloxacin (328.13µg/ml) was the most effective, followed by moxifloxacin (390.63µg/ml) and ciprofloxacin (437.5µg/ml). However, similar to the planktonic bacteria, norfloxacin (875µg/ml) was the least effective against biofilm cells as well. These data conclude that biofilm cells are less susceptible to antibiotics compared to the planktonic cells. It is also evident from these data that some antibiotics are more effective when used against biofilm cells, while others perform better on the planktonic counterparts. Results of this project are expected to provide insight into the efficacy of various fluoroquinolone antibiotics against MRSA biofilms. This study could form the basis for future clinical studies that could recommend special guidelines for management of infections that are likely to involve bacteria in their biofilm state.

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