International
Pharma Conference and Expo

May 2-4, 2018 | Rome, Italy.

Program Schedule

  • Keynote Speaker

    Time:

    Title

    Title: Ovarian Cancer Diagnosis through Genome-scale Expression

    Antonio Scilimati
    University of Bari, Italy.
    Biography
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    Biography

    Antonio Scilimati
    University of Bari, Italy.

    Antonio Scilimati graduated cum laude in Chemistry at the University of Bari (Bari, Italy). PhD at the University of Wisconsin (Madison, USA). Four years as a Qualified Person at MerckSerono plant producing recombinant drugs. Currently, he is an Associate Professor of Medicinal Chemistry at the University of Bari. His scientific interest focus the "pharmaceutical sciences", targeting the cyclooxygenase (COX)-1 as a novel theranostic biomarker in oncology and neuroinflammation.



    Abstract
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    Abstract

    Antonio Scilimati
    University of Bari, Italy.

    Abstract Epithelial ovarian cancer (EOC) is the most lethal gynaecologic malignancy. The global EOC burden is approximately 225,000 new cases per year, with a survival rate of 30%. EOC is hallmarked by a high degree of heterogeneity. This heterogeneity is apparent in tumor histopathology such as serous, mucinous, endometrioid and clear cell histotypes. The high-grade serous ovarian cancer (HGSOC) accounts for approximately 70% of all ovarian carcinoma. Patients with HGSOC show diverse clinical outcomes and usually low survival rates (LSRs), even after the same or very similar treatment regimens. This LSR would also be ascribed to the diagnosis usually made in advanced stage, because no or specific symptoms are related to EOC onset and progression. Early diagnosis determines the patient overall survival. Currently, CA 125 and HE4 are used as early stage EOC biomarkers. Unfortunately, they are not EOC specific and have a poor diagnostic rate, so that the EOC biomarker research is still a challenge. In this presentation, we will show a comparison between the transcriptome profiles of hundreds of HGSOCs and normal tissues, obtained from RNAseq experiments and reported in big data portals. Our analysis resulted in a panel of tenths of genes that are strongly over-expressed in cancer tissues and might be novel candidates as cancer biomarkers. The expression of our selected genes was further tested on a different cohort of patients by comparing matched cancer and normal tissues by the nano-string technology to validate a panel of biomarkers suitable for a very precocious EOC diagnosis.

    Keynote Speaker

    Time:

    Title

    Title: Neuroinflammation and Microglial Constitutive COX-1 Inhibition

    Maria Grazia Perrone
    University of Bari, Italy.
    Biography
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    Biography

    Maria Grazia Perrone
    University of Bari, Italy.

    Researcher of the Department of Pharmacy – Pharmaceutical Sciences of Bari University, Italy. After a stage at the University of Bioverfahrenstechnik (Stuttgart-Germany), her scientific interests have been devoting to clarify the Cyclooxygenases role in inflammation as the earliest step of both neurodegenerative disorders and oncology. Author of approximately 50 scientific publications on international journals and Principal Investigator of scientific projects, among which the grant supporting my current studies [First AIRC Grant-MFAG2015 (Project Id. 17566)].



    Abstract
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    Abstract

    Maria Grazia Perrone
    University of Bari, Italy.

    Neuroinflammation, as the earliest stage of several neurological and neurodegenerative diseases, takes please about 15-20 years before the appearance of specific neurodegenerative clinical symptoms. Among the known mechanisms involved into the neuroinflammatory complex network, the cyclooxygenase-1 (COX-1) (predominantly localized in microglia) plays a previously unrecognized role in the neuroinflammation as demonstrated by the attenuation of the inflammatory response and neuronal loss due to the genetic ablation or pharmacological inhibition of COX-1 activity. The lack of drugs to treat diseases involving the central nervous system (CNS) also resides into the shield exerted by the blood brain barrier (BBB) matrix. BBB has a low permeability, and the development of drugs able to penetrate through its network is one of the challenges of all scientists involved in projecting medicines having active principle ingredients targeting the CNS diseases. A commonly used strategy to overcome this drawback consists to incorporate into the pharmacological active molecule a sugar moiety (i.e. glucose or galactose), in turn capable to “carry” the entire molecule into the CNS by the GLUT-1 carrier, which is located on the membrane of the endothelial cells. In this context, a set of novel compounds endowed with inhibitory activity with cyclooxygenase-1 and GLUT-1 substrate will be presented. Specifically, their design rationale and biological activity will particularly detailed. The work here presented is financially supported by First AIRC Grant-MFAG2015 (Project Id. 17566) “COX inhibitors in conjunction with chemotherapy to target multiple myeloma active disease”.

    Keynote Speaker

    Time:

    Title

    Title: COXs inhibition role in Multiple Myeloma

    Maria Laura Pati
    University of Bari, Italy.
    Biography
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    Biography

    Maria Laura Pati
    University of Bari, Italy.

    Maria Laura Pati graduated cum laude in Medicinal Chemistry at the University of Bari (Italy). PhD at the University of Bari (Italy). One year as Visiting Researcher at Washington University in St. Louis (USA) working on the in vitro and in vivo efficacy of novel molecules for resistant tumors treatment. One month as Visiting Researcher at University of Vienna where she gained expertise on confocal microscopy analysis. Currently, she is granted by First AIRC Grant-MFAG2015 (Project Id. 17566) for the project “COX inhibitors in conjunction with chemotherapy to target multiple myeloma active disease”.



    Abstract
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    Abstract

    Maria Laura Pati
    University of Bari, Italy.

    Multiple Myeloma (MM) is an incurable malignant disease of plasma cells. PGE2 and other prostaglandins (PGs), synthesized by cyclooxygenase (COX)-mediated arachidonic acid transformation, are crucial mediators of inflammation and angiogenesis, and support the growth of several tumors. Two COX isoforms have been identified, COX-1 and COX-2. Despite considerable data concerning COX expression in solid tumors are available, their role in hematologic malignancies and in MM has been little investigated. Non-steroidal anti-inflammatory drugs (NSAIDs), mainly acting as COX inhibitors, have shown to be immunotherapeutic agents in several malignancies, including hematological tumors and MM. Some studies proven the usefulness, in the treatment of MM, of COX inhibitors like indomethacin, ibuprofen, NS-398, celecoxib endowed with a different grade of selectivity towards the inhibition of the two COX isoforms. The pharmaceutic effect of SC-560, Mofezolac, as selective COX-1 inhibitors, Celecoxib, as a selective COX-2 inhibitor, and Aspirin, Ibuprofen with a different grade of selectivity towards COX isoforms, have been evaluated by us on cellular COX status (protein expression, and enzymatic activity) of widely used hMM cell lines (i.e., U937, RPMI-8226, HPC, ARH77). COX-1 and COX-2 role in MM active disease, as well as the usefulness of their selective or non-selective inhibitors to be used as therapeutic agents to strength the action of the clinically used anticancer drugs (i.e., dexamethasone, bortezomib and thalidomide) will be presented. The work here presented is financially supported by First AIRC Grant-MFAG2015 (Project Id. 17566).

    Keynote Speaker

    Time:

    Title

    Title: New Advances in Drug Target Selection and Pharmacogenetics

    Tahani Alrahbeni
    Riyadh Colleges, Saudi Arabia
    Biography
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    Biography

    Tahani Alrahbeni
    Riyadh Colleges, Saudi Arabia

    Tahani Alrahbeni has completed her PhD from Aberdeen University, UK, in Molecular Toxicology and Genetics. Prior to it, she acquired her Masters in Neuropharmacology and Bachelors in Pharmacy from King Saud University, Saudi Arabia. Currently, a postdoctoral staff at King Faisal Specialist Hospital and Research Center, Immunocompromised Department. She is the Vice Dean at Riyadh Colleges and Dentistry and Pharmacy, in addition to being the Clinical and Administrative coordinator at the Postgraduate department. She has published a number of articles on neuronal genetics and neurological disorders, HIV & AIDS in addition to Public health articles.



    Abstract
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    Abstract

    Tahani Alrahbeni
    Riyadh Colleges, Saudi Arabia

    One of the most pressing issues facing the pharmaceutical industry is the decreased rate of lead drug targets. Genomics and proteomics are today well established in drug discovery and development is helping to bring forward a matchless number of potential lead drugs. Over the last two decades, several new genomic technologies have been developed in hopes of addressing the issues of target identification and lead drug targets optimization. Proteomics is a technology platform that is gaining widespread use in drug discovery and drug development programs. Defined as the protein complement of the genome, the proteome is a varied and dynamic repertoire of molecules that in many ways dictates the functional form that is taken by the genome. Furthermore, Personalized Medicine is more than just a metabolic activity of a person. Pharmacogenomics, pharmacogenetics, pharmacoproteomics, and metabolomics play an important role in the development of personalized medicines. Personalized medicine uses information about a person’s genes, proteins, enzyme activities, and cellular environment to diagnose and treat disease, such as cancer, chronic pain and neurological disorders. A major problem of personalized medicine is its high coast. This technology could play a significant role in defining the dosage setting for subsets of the population. The success of the personalized therapy is possible through the application of technology, which can provide a bridge between metabolism status and an individual’s response to a particular drug and therapeutic modality.

    Sessions:
    Pharmacology,Clinical Pharmacology & Molecular Pharmacology

    Time:

    Title: Sulfasalazine/Dasatinib: A Novel Combined Therapy in Hepatocellular Carcinoma Cell Line

    Marium
    Arab Academy for Science, Technology and Maritime Science, Egypt

    Biography
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    Biography

    Marium
    Arab Academy for Science, Technology and Maritime Science, Egypt

    Marium Shamaa; born 1986, graduate of Elekbal Elkawmeya language school in Alexandria (class 2002), studied pharmaceutical science (2003–2007) at Alexandria University,Egypt. She received her master degree (2012) in Pharmacogenomics (IGSR,Alexandria University) and PhD degree (2016) in Biochemistry and Molecular Biology (IGSR,Alexandria University). She is studying the Clinical Pharmacotherapy Course at the active training company. She is now Lecturer of Biochemistry and Molecular Biology, college of Pharmacy, Arab Academy for Science, Technology and Maritime Science,Alexandria,Egypt and simultaneously a member in the editorial board of the Universal Journal of Pharmaceutical Research. Her professional interests focus Pharmacotherapy, Pharmacogenetics, Oncology and Liver diseases.



    Abstract
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    Abstract

    Marium
    Arab Academy for Science, Technology and Maritime Science, Egypt

    Hepatocellular carcinoma (HCC) is one of the most common human malignancies. Lack of efficient therapy for advanced HCC is a pressing problem worldwide. Dasatinib is an oral dual BCR/ABL and Src family tyrosine kinase inhibitor approved for use in patients with chronic myelogenous leukemia (CML). The main targets of dasatinib, are BCRABL, SRC and GFR. NFκB is one of widely recognized positive regulator of cancer cell proliferation. It could be worthy to combine multikinase inhibitor with NFκB inhibitor for targeting of multiple signaling pathways involved in carcinogenesis. This study aimed to evaluate the potential anti-carcinogenic effects of either dasatinib and/or sulfasalazine on HEPG2 cell line as a model of HCC. The current study was conducted on 4 groups. For determination of the specific doses for the selected drugs MTT assay was done. While for laboratory investigations; cell lysates and nuclear extracts were subjected to ELISA and QRT-PCR. Both drugs have modulated RAF/ERK, BCR/ABL and NFκB pathways. Additionally, sulfasalazine confirmed its antiproliferative effects by lowering cyclin-D1, its apoptotic effect by improving the level of caspase-3, and its antiangiogenic effect by lowering VEGF level in a pattern similar to that of dasatinib. On the molecular level, dasatinib and sulfasalazine downregulated the gene expression level of c-MET. On almost all the parameters, the sulfasalazine effects were more superior when compared to that of dasatinib, while the combination regimen showed the best effects. The author present study showed for the first time the beneficial anticarcinogenic effects of dasatinib and sulfasalazine coadministration on HCC cells.

    Time:

    Title: Glitazones to Gliptins and Gliflozins: Quest For Cardio-Friendly Antidiabetics

    Kiran Dubey
    Jamia Hamdard University, India

    Biography
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    Biography

    Kiran Dubey
    Jamia Hamdard University, India

    Dr. Kiran Dubey (M. Pharm, Ph.D. PGDMM) is currently associated with Jamia Hamdard as Assistant Professor in the Department of Pharmacology, School of Pharmaceutical Education and Research, New Delhi. She has also served in the Medical Information Department of Ranbaxy Laboratories, Systopic Laboratories, Dee Pharma Ltd and Skin Institute and School of Dermatology. Her areas of research include cardiovascular safety profile of NSAIDs, diabetes and related complications. She has guided twenty four post graduate students and three Ph.D. students in the field of Pharmacology and Pharmacy Practice, published articles and has been reviewer for International and National journals of repute.



    Abstract
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    Abstract

    Kiran Dubey
    Jamia Hamdard University, India

    The well-characterized associations between type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) are twofold. First, CVD is the underlying cause of more than 60% deaths in diabetic patients. Second, the hyperglycaemia of T2DM, as measured by the percentage of glycosylated haemoglobin, is believed to increase the risk of coronary artery disease and myocardial infarction (MI). Given this relationship, physicians managing T2DM must remain aware of the cardiovascular effects of their therapeutic choices. Unfortunately, most oral antidiabetic therapies made available to date have not resulted in reduced incidences of MI, stroke or cardiovascular death in diabetic subjects. While metformin appears to be safe, it may lead to lethal lactic acid acidosis, especially in patients of recent MI or heart failure. An increased risk of all-cause mortality and cardiovascular related mortality has been associated with sulfonylureas and their combination with metformin. Meglitinides effects are similar to those of sulfonylureas, due to their analogous mechanism of action. Glitazones are unsafe in NYHA class III or IV due to weight gain and oedema. The cardiovascular safety outcome trials conducted with dipeptidyl peptidase-4 (DPP-4) inhibitors, saxagliptin and alogliptin showed an increase in hospitalization due to heart failure, while sitagliptin demonstrated no such effect. Evidence for cardiovascular benefits of glucagon-like peptide-1 (GLP-1) receptor agonists has been similarly heterogeneous, with liraglutide and semaglutide reducing the risk of composite cardiovascular outcomes, but lixisenatide having no reduction or increase in cardiovascular risk. The trial conducted with sodium-glucose co-transporter-2 (SGLT2) inhibitor, empagliflozin found it to be superior in reducing major cardiac events. However, there was a non-significant increase in silent MI with empagliflozin. While the FDA regulatory mandate to demonstrate the cardiovascular safety in order to approve new antidiabetic drugs, the quest for cardio friendly antidiabetics continues.

    Time:

    Title: Th17 and IL-17 Regulatory Pathways in Chronic Inflammation Progression and Autoimmune Diseases Therapy

    Francesco Maione
    University of Naples Federico II, Italy

    Biography
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    Biography

    Francesco Maione
    University of Naples Federico II, Italy

    (1) D'Acquisto F, Maione F, Pederzoli-Ribeil M. From IL-15 to IL-33: the never-ending list of new players in inflammation. Is it time to forget the humble aspirin and move ahead? 2010 Feb 15;79(4):525-34. doi: 10.1016/j.bcp.2009.09.015. (2)Beringer A, Noack M, Miossec P. IL-17 in Chronic Inflammation: From Discovery to Targeting. Trends Mol Med. 2016 Mar;22(3):230-41. doi: 10.1016/j.molmed.2016.01.001. Epub 2016 Jan 31. (3) Maione F. ommentary: IL-17 in Chronic Inflammation: From Discovery to Targeting. Front Pharmacol. 2016 Aug 11;7:250. doi: 10.3389/fphar.2016.00250. (4) Robert M, Miossec P. Effects of Interleukin 17 on the cardiovascular system. Autoimmun Rev. 2017 Sep;16(9):984-991. doi: 10.1016/j.autrev.2017.07.009. Epub 2017.



    Abstract
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    Abstract

    Francesco Maione
    University of Naples Federico II, Italy

    Interleukin-17A (IL-17A) is the most studied member of the IL-17 cytokine family. It is mainly produced by T-helper (Th)-17 lymphocytes, and also by cytotoxic CD8+ T cells (Tc17), γδT cells (γδ-17), natural killer T (NKT) cells and neutrophils (1). This cytokine elicits protection against extracellular bacterial and fungal infections and, when produced in excess, it contributes to chronic inflammation (2). Since its discovery, much attention has been given to mediators and factors responsible for the development of IL-17-producing cells while very few studies have investigated the inflammatory properties of this cytokine and signaling pathways involved in its physio-pathological functions. A growing number of evidences gathered over the last few years indicate that IL-17A might play a key role in sustaining chronic inflammation in patients suffering from autoimmune diseases (such as rheumatoid arthritis, psoriasis, multiple sclerosis and some bowel inflammatory diseases) and probably in the hemostatic disorders observed in these patients (3,4). Here, I summarize the most recent findings on the biological effects of these new inflammatory cytokine and discuss how these discoveries might influence our current view on therapeutic approaches to treat ongoing inflammation and inflammatory-related diseases.

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    Title: Androgen Effect on The Muscular Expression of Neurotrophins; New Pharmacological Targets and Possible Modulators

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Biography
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    Biography

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Dr Nasr Alrabadi has completed his bachelor degree in medicine (2009) and received the Jordanian board for practicing medicine (2010). Dr Alrabadi completed his master degree in pharmacology (2012) and has been awarded his PhD in medicine and nanotechnology (2016) from Sydney University. Currently, he is assistant professor at the department of Pharmacology/ faculty of medicine, Jordan University of Science and technology (JUST) and a team leader for the molecular biology and analytical facility at the Nanotechnology institute/ JUST University. As a young researcher, he has 7 publications/ abstracts.



    Abstract
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    Abstract

    Nasr Alrabadi
    Jordan University of Science and Technology, Jordan

    Androgens are natural and essential hormones in the development and function of body organs. As well, these hormones can be manufactured and administered to the body aiming to treat different pathological conditions. Anabolic steroids are forms of these manufactured androgens which are excessively used by body builders aiming to gain excessive muscle weight and strength. However, the abuse of anabolic steroids is associated with variety of catastrophic complications. This dose-dependent effect especially with the differences in the prevalence and prognosis of androgen related diseases between different sexes, can indicate to dose and sex-dependent paradoxical effects of these hormones. Our group focus on studying the effect of wide range of androgen (sustanon) dosages/ concentrations on the differential expression of the main and well-known neurotrophins, so called; BDNF, NGF, NT3 and NT4/5. This expression is studied at the level of the heart, skeletal muscles and gastrointestinal wall (muscles) of either male/ female rats or chickens. Exploring this relationship will strengthen our understanding regarding the paradoxical effect of androgen usage among athletes. On the other hand, it may identify possible pharmacological targets and modulators for variety of pathological conditions related to what we call “androgen-neurotrophins axes”.

    Time:

    Title: Role Of Cellular Bio-Markers In Pharmacotherapy For Cancer Detection And Prognosis

    Vanitha Samuel
    Annamalai University, India

    Biography
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    Biography

    Vanitha Samuel
    Annamalai University, India

    Professor Dr. Vanitha Samuel is a Medical graduate from Stanley Medical College,(1986-1991) Chennai,Tamil Nadu, India .She has completed her Master degree in Pharmacology (1999) at Madras Medical College, Chennai and her Ph.d in Pharmacology at Rajah Muthaiah Medical College , Annamalai University, Chidambaram , where she is currently working as Professor in the Department of Pharmacology . She is a member of the Pharmacovigilance Committee in RMMC and her speciality and field of research includes Toxicology related drug effects and study of drugs that ameliorate them. Her professional interests involves the pre-clinical evaluation of various flavonoid compounds in some selective cancers.



    Abstract
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    Abstract

    Vanitha Samuel
    Annamalai University, India

    The two elements of key importance in the treatment of cancer includes early correct diagnosis and effective interventional therapy .Predicting the response to pharmacological intervention is an optimal goal for healthcare professionals especially in cancer prognosis. Metastasis of cancer indicates a morbid state resistant to standard pharmacotherapeutic interventions. A terminology ‘Companion Diagnostics ’improves predictability of outcomes and holds the promise as a strategic tool for the oncologist to decide which drug would be best suited to treat a particular cancer. Immunohistochemistry (IHC) has emerged as a reliable and sensitive investigative tool that provides supplemental information to the routine morphological assessment of tissues. The use of IHC to study cellular markers that define specific phenotypes has provided important diagnostic ,prognostic and predictive information relative to the disease status and normal cellular biology. Application of antibodies to the molecular study of tissue pathology has been refined and improvised , particularly for use in fixed tissues .In contrast to solution based immunoassays that detect relatively abundant native proteins , the preservation of antigen in fixed tissues is variable and unpredictable . Evolution of newer techniques has resolved these issues and has helped detect proteins in tissues with great sensitivity .It also provides a semi-quantitative assessment , with the ultimate goal of integrating tissue based analysis with proteomic information. Targeted therapies have created a need for a more profound quantitative biomarker information. These newly launched IHC tests are termed as ‘prognostic markers’, ‘predictive markers’ and “advanced personalized diagnostics”.

    Time:

    Title: More than Three Decades of Proton Pump Inhibitors

    Abu Freha Naim
    Soroka University Medical Center, Israel

    Biography
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    Biography

    Abu Freha Naim
    Soroka University Medical Center, Israel

    I received my M.D. from the Tuebingen University, Germany at 2005 before becoming resident at internal medicine and then completed my gastroenterology residency at the Soroka Medical Center at 2014. I am employed as a senior physician at the Institute for Gastroenterology and Hepatology, Soroka University Medical Center and lecturer at the faculty of Health science, Ben-Gurion University, Beer-Sheva, Israel. I deal with gastroenterology and hepatology in general, and especially with genetic syndrome of the colorectal cancer. I researched different topics in the gastroenterology/Hepatology and different issues regarding the Bedouin Arab minority in southern Israel. I am one of the founders group of the Arab Medical Associations in the Negev (AMAN) and the first Chairman of the Associations since 2015.



    Abstract
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    Abstract

    Abu Freha Naim
    Soroka University Medical Center, Israel

    The lecture will include a review of this medications group, including the history and development of the different type of proton pump inhibitors, the differences between the different PPIs in term of pharmacodynamic and pharmacokinetic features. Indications of treatments, efficacy of the drugs and the doses of the different PPIs and route of taking will be a part of the lecture. The PPIs were considered as safe medications, however in the last decade the are more and more reports regarding different side effects, in the short term and long-term use. The talk will include a comprehensive review of the different reported side effects in the updated literature. Important side effects will be included in the talk, osteoporosis, fracture risk, gastrointestinal infections, pneumonia, iron deficiency anemia, vitamin B12 deficiency, hypomagnesemia and increasing risk of polyps and gastric cancer. Another issue will be included in the lecture will be the updated recommendation regarding use of proton pump inhibitors during pregnancy and during breastfeeding. What about drug-drug interaction? and what about patients with chronic renal failure or liver cirrhosis? Should the dose be adjusted? These questions will be answered during the talk. The talk will be closed with the best practice advices regarding these drug group.

    Time:

    Title: Involvement of Inflammation in the Pathophysiology and Treatment of Mood Disorders

    Abed N. Azab
    Ben-Gurion University of the Negev,Israel

    Biography
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    Biography

    Abed N. Azab
    Ben-Gurion University of the Negev,Israel

    Dr. Azab completed his Ph.D. in pharmacology in the Department of Clinical Pharmacology in Ben-Gurion University of the Negev (Israel), focusing on the study of anti-inflammatory drugs. Subsequently, as a post-doctoral fellow in Wayne State University (Michigan), Dr. Azab investigated the mechanisms of mood-stabilizing drugs, focusing on the role of GSK-3 in inositol biosynthesis. Currently, Dr. Azab is an assistant professor in Ben-Gurion University of the Negev. Major research projects in his lab: 1) Studying the role of inflammation in the pathophysiology and treatment of mood disorders; 2) searching for novel therapeutic strategies for mood disorders.



    Abstract
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    Abstract

    Abed N. Azab
    Ben-Gurion University of the Negev,Israel

    Introduction. Accumulating data suggests that inflammation plays a role in the pathogenesis and treatment of mood disorders. Consistently, classic anti-inflammatory drugs exerted beneficial effects in randomized clinical trials of mood disorders patients. Moreover, psychotropic drugs possess anti-inflammatory effects. However, despite these supporting findings, many contradicting results have also been reported. Aims. (1) We investigated the effects of chronic treatment with psychotropic drugs on bacterial lipopolysaccharide (LPS)-induced inflammation in rats; (2) we tested the efficacy of mechanistically-different anti-inflammatory compounds in behavioral models in rats. Materials and Methods. (1) Rats were treated with psychotropic drugs (carbamazepine, lithium, haloperidol, and imipramine) for 4 weeks through a daily intraperitoneal (ip) injection. On day 29, rats were injected with vehicle or LPS. At 2 hours later, rats were sacrificed, blood was collected and different brain regions were excised. Levels of inflammatory constituents in plasma and brain were examined by ELISA. (2) Rats were treated (ip) for 2 weeks with one of the following anti-inflammatory compounds: dexamethasone, a potent corticosteroid; nimesulide, a selective cyclooxygenase-2 inhibitor; montelukast, a leukotrienes receptors antagonist; pentoxifylline, a tumor necrosis factor- inhibitor; and, JSH-23, a selective inhibitor of nuclear factor- B. At the end of drug treatment, animals were subjected to a battery of behavioral tests. Results. Psychotropic drugs treatment resulted in both anti- and pro-inflammatory effects. Some of the drugs prominently affected the levels of particular inflammatory mediators in the plasma and specific brain regions. Similarly, the various anti-inflammatory compounds differently affected the behavioral phenotypes of rats, showing both therapeutic and negative effects. Conclusions. Our results add to the ambiguity regarding the role of anti-inflammation as a therapeutic strategy for mood disorders. It is important to elucidate the therapeutic potential of mediator/pathway-specific anti-inflammatory compounds in randomized clinical trials.

    Time:

    Title: Kinetic Target-Guided Synthesis: a MS-Based Fragment Evolution Platform

    Roman Manetsch
    Northeastern University, USA.

    Biography
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    Biography

    Roman Manetsch
    Northeastern University, USA.

    Dr. Roman Manetsch received his PhD in Chemistry in 2002 from the University of Basel under the guidance of Wolf-Dietrich Woggon. He joined the group of K. Barry Sharpless at the Scripps Research Institute working on click chemistry. In 2005, he moved to the University of South Florida and established a research group focusing on medicinal chemistry. In 2014, Dr. Manetsch assumed a position of an Associate Professor at Northeastern University. His current research focuses on lead discovery and optimization using synthetic chemistry in close conjunction with mass spectrometry.



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    Abstract

    Roman Manetsch
    Northeastern University, USA.

    The Manetsch laboratory focuses on the development and implementation of LC/MS-based lead discovery and optimization platforms. Herein, the development of kinetic Target-Guided Synthesis (TGS) and its implementation for the identification of small molecules modulating protein-protein interactions will be presented. In kinetic Target-Guided Synthesis (TGS), the biological target is actively engaged in the irreversible assembly of its own inhibitory bidentate ligand from a pool of smaller reactive fragments. The screening method can be as simple as determining whether or not the inhibitory product has been formed in a given test mixture. To date, kinetic TGS has exclusively been applied to enzymatic targets and these TGS applications have been successful because of a unique combination of (a) the slow nature of the chemical reaction combining the two fragments into a single molecule and (b) the use of reactive fragments showing moderate to high affinity towards binding pockets of the enzyme. Compared to kinetic TGS screening of enzymes, however, the discovery of inhibitory compounds against "undruggable" targets is more challenging and thus requires major modifications over the existing kinetic TGS approaches. The Manetsch laboratory demonstrated that the sulfo-click reaction, an amidation reaction between thio acids and sulfonyl azides, is suitable for a kinetic TGS approach targeting the proteins of the Bcl-2 family. Furthermore, the use of a triple quadrupole mass analyzer improved the data quality and increased the throughput by approximately 200-fold rendering the platform industrial robustness.

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    Title: Study of factors influencing the medication errors

    Majed Isa
    Taif University, Saudi Arabia.

    Biography
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    Biography

    Majed Isa
    Taif University, Saudi Arabia.

    Citizenship of both Jordan and Belgium Married and have four children. All my high education conducted in Belgium at Brussels and Louvain University. Ph D degree in Pharmaceutical sciences obtained with the highest consideration in 1989 from Brussels University. Director and manager of research projects for many Pharmaceutical industrials in Belgium and Jordan. Prof. of Pharmacology and Toxicology in Jordan, Palestine, Saudi Arabia Universities. Dean of the Pharmacy Faculty, Alqods University- 2001.2003. Director of Academic and Development Administration of King Fahad Hospital in Dammam- KSA- 2008. Teaching staff member in Taif University, Pharmacy Faculty- Department of Pharmacology and Toxicology- 2010. Research interest in Pharmacology of Antibiotics and Anticancer drugs. Metabolism of drugs. Addiction, Biotechnology and environmental pollution. Publication more than 50 Articles, four of them original in different international Journal. Participate in more than 30 conference, symposium and training staff in the world.



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    Abstract

    Majed Isa
    Taif University, Saudi Arabia.

    Medication error is any preventable event that may cause or lead to inappropriate medication use or patient harm while the medication is in control of the health care professional, patient or consumer. Studies done since the 1970's have shown the high incidences of medical errors and deaths resulting from them. Only 3% of physicians believe that medical errors are a principal health concern and yet, there is more concern with car accidents. Medical error statistics in the United States are enormous and alarming. The American medical system is the number one killer in the U.S. In ten years; the deaths caused by conventional medicine are approximately 8 million. The proposal concerns with the determination of errors during the process of treatment such as self-medication, prescribing errors, lack of knowledge, lack of attention, poor drug selection and poor monitoring. The community pharmacy errors such as, wrong quantity, wrong drug, wrong dosage form, wrong strength and wrong information (label). Most of patient errors come from the drug administration, patient personality, education and culture. . Health care cost savings, Improves the image of the pharmacist and helps pharmacy to become a true clinical profession. Teaching patients about their medication and their disease also help to prevent medication errors. Most of countries have a statistical data concerning the medication errors and comparing these data may lead to reduce and detect the parameters of medication errors. This study Investigates the parameters of self-medication in the population and their relationship with the medication errors. The sample size of our study was 600 (n=600); healthcare professions (200) and public (400). The study suggests that participants agree that factors such as, knowledge, information, self-medication and organization have an impact on medication errors with 78.2%, 61.8%, 67.2%, 83.6%, respectively. The rate of medication errors in one tertiary hospital was about 40%.

    Time:

    Title: Effects of Bulgarian Propolis on Cd34+ Cells in Vivo And on two Cell Lines in Vitro

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria.

    Biography
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    Biography

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria.

    Professor Dr. Lyudmil Peychev Peychev, PhD, MHM, is Dean of the Faculty of Pharmacy at the Medical University of Plovdiv, Bulgaria and Head of the Department of Pharmacology and Drug Toxicology. He is a specialist in Pharmacology, Clinical Pharmacology and Therapeutics, Master of Health Management. Professor L.Peychev is the author of scientific articles in the field of neuropharmacology, clinical pharmacology, phytotherapy and apitherapy. He is Head of research projects and author of inventions and trademarks. As a scientist he has won recognition by the academic community in Bulgaria and other countries.



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    Abstract

    Lyudmil P Peychev
    Medical University of Plovdiv, Bulgaria.

    Purpose. To evaluate whether Bulgarian propolis augment the release of hematopoietic stem cells from bone marrow into the blood stream of rats and to analyze its effect on cell proliferation and surviving in vitro. Methods. Twenty male Wistar rats were divided into two groups (n=10) and treated as follows:1-st group (Controls) – aqua destillata, p.o.; 2-nd group – Bulgarian propolis in a dose 100 mg/kg bw, i.p. Two hours after single administration of the substances blood samples were analyzed by flowcytometry for CD34+ and white blood cells differential were performed using hematology analyzer. Peripheral blood mononuclear cell (PBMC) and Mouse lymphoma cell line L5178Y were cultivated in 96 well plate in culture medium RPMI-1640 with 100 U/mL Penicillin and 100 mg/mL Streptomycin. 10% Fetal bovine serum was added to the L5178Y cell line. The cells were grown in an incubator at 37оС and 5% СО2 for 24 hours and were treated with increasing propolis concentrations - 0,01; 0,1; 1,0 and 10 mg/L. To analyze the effect of the natural product on cell viability, a cytotoxic MTT-test was used. Results. Propolis mobilized significant amount of hematopoietic stem cells in the blood stream versus controls. It also caused a significant increase in the number of leukocytes and lymphocytes in rats in comparison to controls. In human PBMC cells treated with propolis in concentrations 0,01; 0,1; 1,0 and 10 mg/L the cell vitality was higher in comparison with the control cells. The percentage of living cell was increased from 106 to 135% with increasing of the propolis concentrations. In propolis treated tumor cells L5178Y the percentage of living cells decreased with increasing of propolis concentrations. The results of 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MMT) assay showed that concentration 0,1 mg/L leads to 73% cell survival, 1,0 mg/L tо 69%, and the highest concentration – 10 mg/L decreased the percentage of surviving cells to 40% . Conclusion. Bulgarian propolis has no effect on the stem cells maturation but mobilize the rats stem cells in peripheral blood. In human PBMC the proliferative activity after treatment with propolis suppose the ability to influence cell differentiation. From the other side the cytotoxic effect of propolis on the tumor cell line determines its potential in anti-tumor therapy. Keywords: CD34+ cells, cell lines, proliferation, rats, Bulgarian propolis.

    Sessions:
    Pharmaceutical Chemistry

    Time:

    Title: Transition Metal Complexes/Organometallic Compounds As Anticancer/Anti HIV Drugs Or In Pharmaceutical Industry

    Prakash kinthada
    JNTU University, India.

    Biography
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    Biography

    Prakash kinthada
    JNTU University, India.

    Prakash Kinthada is a Professor in Chemistry at Sri Vidyanikethan Engineering college, JNTU University in Ananthapur, A. Rangam Peta, Tirupathi, India.



    Abstract
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    Abstract

    Prakash kinthada
    JNTU University, India.

    Cancer is a dreadful disease and any practical solution in combating this disease is of paramount importance to public health. Cancer patients have burdened by drug induced toxic side effects, and no turned to seek help from the complementary and alternative medicine hoping for a better cure. Research on Platinum based drugs and Non Platinum based drugs is a Multi-Million Dollar Industry in USA and there is every need to produce safe drugs for the cure of this monstrous disease. Flavonoids have a long history of use in traditional medicines in many cultures. The phytochemical, curcumin is one of the major dietary flavonoid, belonging to a group of flavonol, Curcumin is a natural polyphenol. It is highly potential molecule capable of preventing and treating various cancers. Various dietary chemo preventive agents, turmeric powder or its extract are broadly used as therapeutic preparations in Indian System of medicine. We provide a summarized synthesis and structural determination of Curcumin Oxime, Curcumin Thiosemicarbazone derivative of Gold (III) complex. The use of these analogs for prevention of cancer tumor progression and treatments of human malignancies. A pharmacologic agent for treating and/or preventing cancer, among other diseases and conditions, and particularly breast, prostate, and pancreatic cancer, in humans and animals. The novel pharmacologic agent is an isoflavonoid or isoflavonoid mimetic covalently attached to a cytotoxic pharmacophore that, preferably has the ability to conjugate with a metal salt to form a more potent metal complex, particularly a Au (III) complex and other complexes of Platinum, Palladium, Ruthenium, Copper etc. My talk would mainly encompass different Transition Metal Complexes/Organometallic Compounds that are presently used as drugs, especially Anticancer and Anti-HIV drugs, apart from Anti-inflammatory, Antimicrobial, Antibacterial and diseases like Arthritis and Parkinson’s Disease etc. The talk would mainly focus on the use of Medicinal Chemistry and it’s application to Drug Design and Development in Pharmaceutical Industry , especially Transition Metal Complexes and Organometallic Compounds viz. Gold, Platinum, Palladium And Ruthenium apart from Copper, Cobalt, Iron, Nickel, Zinc, Cadmium etc. The main emphasis of my talk would be on Different class of Ligands, their Schiff’s Bases and Transition Metal Complexes especially Au, Pt, Pd and Ru, with the main aim of designing, developing very novel small molecules, as possible and extremely potential candidates as Anti-cancer and Anti-HIV drugs. The talk would provide an overview of current programs being undertaken in our laboratories, especially focused on the development of potent ligands capable of recognizing Binding sites and diverse strategies employed by my group for elucidation of Anti-Cancer and Anti-HIV drug Leads to Circumvent the problem caused by Cis-Platin. We have synthesized and characterized several phytochemicals from Traditional Medicinal Plants and isolated some phytochemicals and made the corresponding Oximes, Thiosemicarbazones and Substituted thiosemicarbazones as ligands and synthesized, characterized, structurally elucidated their Transition Metal Complexes especially with Gold, Platinum, Palladium, Ruthenium, Copper etc. and Studied their Anticancer Activity, Nuclease activity etc. and tested their potential as Anticancer Drugs. The main aim of our extensive/preclinical Pharmaceutical development program is to investigate the use of these extremely novel small molecules-metal complexes/compounds of phytochemicals, flavanoids etc., which have very interesting structural features and properties and hence are excellent candidates as Anti-Cancer and Anti-HIV drugs .The main aim of our research is Design ,Development and Synthesis of Transition Metal Complexes/ Organometallic Compounds that would certainly help to bring this force of nature from BENCH to BEDSIDE and enhance Cancer Killing with less toxic effects and would certainly lead to initiation of clinical trials.

    Time:

    Title: Design and Synthesis of Ligands for G Protein-Coupled Receptor-1

    Thet-Thet Htar
    Monash University Malaysia, Malaysia.

    Biography
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    Biography

    Thet-Thet Htar
    Monash University Malaysia, Malaysia.

    Dr. Htar is an academic lecturer and researcher in School of Pharmacy, Monash University Malaysia. She graduated with Bachelor of Pharmacy (Honours) in 1999 from the University of Wales, Cardiff where she successfully completed her PhD in 2004. Her current research interest is in the area of drug design and synthesis of small molecules with potential anti-cancer activity for the treatment of breast cancer. Her research interest has been extended to chemistry of natural products and synthesis of natural products based analogues. She has published a number of research and review articles in the area of synthesis and natural products.



    Abstract
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    Abstract

    Thet-Thet Htar
    Monash University Malaysia, Malaysia.

    G protein-coupled estrogen receptor-1 (GPER-1, formerly known as GPR30) is a novel membrane receptor, which is noted to be able to mediate rapid estrogen signaling. It is widely expressed in cancer cell lines and primary tumour of breast, endometrium, ovaries, thyroid, lung, prostate, testicular germ cells and the brain. The high level expression of GPER-1 in breast cancer, ovarian cancer and endometrial cancer has shown association with poor survival. Due to its significant association with numerous physiological functions and breast cancers, it is emerging as a potential therapeutic target. In the search of GPER ligands, a variety of classical ER ligands have been noted to be able to interact with GPER-1 with differences in binding affinities. Some of the traditionally known ER antagonists have shown agonistic activity with GPER protein. As X-ray crystal structure GPER protein is not available, it has been a challenge in designing of GPER ligands. In this project, we studied the molecular structure requirement of GPER-1 ligands using homology model of GPER. We reported here approaches that have been used in homology modeling of GPER protein and synthesis of potential GPER ligands.

    Time:

    Title: Synthesis and Biological Evaluation of Some New Thiazolodiazepine Analogues as CNS Active Agent.

    Sara Al-Rashood
    King Saud University, Saudi Arabia

    Biography
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    Biography

    Sara Al-Rashood
    King Saud University, Saudi Arabia



    Abstract
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    Abstract

    Sara Al-Rashood
    King Saud University, Saudi Arabia

    The ultra-short acting activity of ethyl 8-oxo-5,6,7,8-tetrahydro-thiazolo[3,2 a] [1,3]diazepin-3-carboxylate (HIE-124, 3), as a member of a novel class, which might overcome many of the disadvantages and problems that usually associated the use of thiopental or benzodiazepines as intravenous anesthetic agents has been reported. The present investigation is directed toward the synthesis of new derivatives of the parent thiazolo[3,2-a][1,3]diazepine (HIE-124, 3), in continuation to the previous patented efforts in this area. These derivatives possess the potential to exhibit a promising and varying range of CNS activities, including, among others, hypnotic and anticonvulsant activities. The proposed compounds were synthesized according to the designed strategy. Structure elucidation of the synthesized intermediates and final products was attained by the aid of IR, 1H, 13C NMR, and mass spectrometry. The synthesized compounds were evaluated for various CNS activities. The new analogues (E)-Ethyl 3-methyl-8-oxo-5,6,7,8-tetrahydro-thiazolo[3,2-a] [1,3]diazepine-2-carboxylate (15) and (E)-2-Bromo-3-methyl-6,7-dihydro-thiazolo[3,2-a] [1,3]diazepin-8(5H)-one (20) showed marginal hypnotic potency compared to what was reported about the parent compound HIE-124 (12). Biological screening results allowed the allocation of two potent anticonvulsant agents worth patency. Compounds 15 and 20 proved to be the most active compounds in the present investigation as anticonvulsants with remarkable 100% protection against PTZ induced convulsions as compared with the standard drug valproic acid. It is worth mentioning that compounds 15 (0.78 mmole/kg) and 20 (0.39 mmole/kg) proved to be almost two and four fold more active, respectively; than the used positive control sodium valproate (1.38 mmole/kg). Structure activity correlation of the obtained results revealed that, CNS activity is embedded in the structure core (8-oxo-5,6,7,8-tetrahydro-thiazolo[3,2-a][1,3]diazepine) of the investigated compounds. Structure activity relationships (SAR) were deduced from the obtained data. These studied 8-oxo-5,6,7,8-tetrahydro-thiazolo[3,2-a][1,3]diazepine analogues could be considered as useful templates for future development and further derivatization or modification to obtain more potent CNS active compounds.

    Time:

    Title: Comparison of conventional and microwave-assisted synthesis of some novel phthalimide derivatives and evaluation of their anticancer activity

    Tapan Kumar Maity
    Jadavpur University,India

    Biography
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    Biography

    Tapan Kumar Maity
    Jadavpur University,India

    Professor Tapan Kumar Maity, Ex-Head of the Dept of Pharm Technology, Jadavpur University, Kolkata, India has the experience in Teaching and Research over 26 years. He has guided 14 PhD scholars, 25 M. Pharm thesis and 30 B.pharm projects. Recently two centrally funded project works have been completed successfully under his supervision. His area of interest in synthetic medicinal chemistry and natural products targeting cancer and diabetes in different cell lines. Already 85 papers were published in the national and international journal till now. He has visited USA, Austria, Switzerland and The Netherlands.



    Abstract
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    Abstract

    Tapan Kumar Maity
    Jadavpur University,India

    A series of novel phthalimide derivatives were synthesized from various 4-Amino -5-mercapto-3-(substituted phenyl)-1, 2, 4-triazole derivatives by fusion of phthalic anhydride. Although synthetic method is a multistep procedure but final step of synthesis was done by conventional and microwave irradiation method for comparative study. The structures of final synthesized compounds were confirmed by using FT-IR, 1HNMR, LC-MS and in vivo anticancer activity was evaluated on the Ehrlich Ascites Carcinoma (EAC) bearing mice model. Here, EAC cell was used as tumor cell for the present study. All synthesized compounds were given at a dose of 100mg/kg; body weight orally. 5-Fluorouracil (20 mg/kg; body weight intraperitoneally) was used as standard drug. From the present study, it can be concluded that triazole containing phthalimide derivatives showed significant (P<0.01) anticancer activity compared to EAC control group and microwave irradiation method was much better than conventional reflux method because microwave irradiation decreased the reaction time by improving the yield value. Keywords: Synthesis, conventional, microwave, EAC cell, anticancer activity

    Sessions:
    Pharmaceutical Nanotechnologies

    Time:

    Title: Engineering Smart Nanoparticles for Targeted Cancer Therapy

    Wafa Al-Jamal
    Queen's University Belfast, UK.

    Biography
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    Biography

    Wafa Al-Jamal
    Queen's University Belfast, UK.

    Dr Al-Jamal is an overseas and a UK-registered pharmacist. She completed her PhD in Drug Delivery and Nanomedicine in 2008 at UCL School of Pharmacy, London. She is currently a Reader in Drug Delivery and Nanomedicine at The School of Pharmacy, Queen’s University Belfast, UK. She is also a Prostate Cancer Research Fellow working on developing novel nanomedicine to treat advanced prostate cancer in men. She worked at the University of Ease Anglia, Norwich, as a Lecturer in Drug Delivery and Nanomedicine (2013-2017), after working as a Senior Research Fellow at University College London and King’s College London (2009-2013). Dr Al-Jamal’s main research interests focus on engineering novel nanomaterials for biomedical applications. Her current research, in Cancer Nanomedicine, aims to design smart vectors to deliver a wide range of therapeutic agents and targeting moieties, and to fabricate multifunctional nanoparticles for combinatory therapy and theranostic applications. Her long-term research career is to facilitate the translation of nanoparticle-based therapeutics from the lab to the clinic. Wafa is the GSK Emerging Scientist Award winner for 2015, and Gro Brundtland Award winner for 2017. She has published over 40 papers in high impact journals. Currently, she is a Visiting Professor at Guizhou Medical School, China.



    Abstract
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    Abstract

    Wafa Al-Jamal
    Queen's University Belfast, UK.

    Most cancer chemotherapeutics lack tissue specificity, resulting in many undesirable side effects. Selective drug delivery to the tumour tissues could ultimately increase local drug concentrations at the tumor without the need to escalate the administrated doses in patients. A wide range of drug delivery systems has been developed to alter the pharmacokinetics of drug molecules, and enhance their tumour targeting. Furthermore, several approaches have been explored to increase the bioavailability of drugs at the site of action, utilising the unique characteristics of the tumour microenvironment, such as overexpressed enzymes, acidic pH, and hypoxia, or using external triggers, such as heat, ultrasound, and light. In this talk will describe the latest delivery systems that we have developed in our laboratory to enhance the tumour accumulation of anticancer drugs, utilising internal and external triggers.

    Time:

    Title: A novel oral drug delivery system for AMB

    Bushra AlQuadeib
    King Saud University, Saudi arebia.

    Biography
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    Biography

    Bushra AlQuadeib
    King Saud University, Saudi arebia.



    Abstract
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    Abstract

    Bushra AlQuadeib
    King Saud University, Saudi arebia.

    Objectives: Oral AMB delivery system is is the utmost invention to overcome its adverse effects and nephrotoxicity for better patient continence’ and minimal hospitalization time. Therefore, the feasibility of selected formulations of AMB loaded to PEGylated polylacic-polyglycolic (PLGA-PEG) nanoparticles (NP) as novel oral AMB delivery system in rats with and without an absorption enhancer, glycyrrhizic acid (GA), was investigated. Methods: The absolute and relative bioavailabilities of AMB from the selected formulations were conducted in nine groups of rats (n=6), using Fungizone® oral dose as reference standard. Results: A novel oral drug delivery system was developed for AMB. Oral administration of AMB loaded to PLGA-PEG NP to rats resulted in considerable oral absorption into the systemic circulation compared to Fungizone®. The addition of 2% of GA to the AMB formulation significantly (P<0.05) improved the bioavailability to 10.5% and the relative bioavailability was > 790% than that of Fungizone®. Conclusions: Oral regimen of AMB-loaded to PLGA-PEG NP formulation to rats was achieved with significant oral absorption. Further investigation is needed in the area of low bioavailability drugs. Keywords: Amphotericin B; Oral delivery; Nanoparticles; Bioavailability; Toxicity Objectives: An effective oral treatment of amphotericin B (AMB) is the utmost invention of its delivery system to overcome its adverse effects and nephrotoxicity for better patient’s quality of life and minimal hospitalization stay. Therefore, the in vitro efficacy and toxicity and in vivo nephrotoxicity of selected formulations of AMB loaded to PEGylated polylacic-polyglycolic (PLGA-PEG) nanoparticles (NP) as novel oral AMB delivery system in rats, after single and multiple administrations, were investigated. Methods: The toxicity of AMB in these formulations was examined by in vitro blood hemolysis test and the in vivo nephrotoxicity was carried out in five groups of rats (n=3) dosed as 1.0 mg/kg via iv route after single and multiple dosing (once daily) for a week by measuring the blood urea nitrogen (BUN) and plasma creatinine (PCr). The in vitro AMB antifungal activity of these formulations on C. albicans was also assessed. Results: A novel oral drug delivery system was developed for AMB. The developed AMB formulations show minimal toxicity and better efficacy compared to Fungizone®. No nephrotoxicity, in rats, was developed after a week of multiple dosing of AMB NP as the BUN and PCr were within the normal levels. Conclusions: Oral regimen of AMB-loaded to PLGA-PEG NP formulation to rats was achieved with significant efficacy and minimal toxicity. Further investigation is needed in the area of low bioavailability drugs. Keywords: Amphotericin B; Nanoparticles; PLGA-PEG; Efficacy; Nephrotoxicity

    Sessions:
    Pharmaceutical Technology

    Time:

    Title: Non-invasive NaV measurements using molecular imaging

    Matthias Schoenberger
    University of Leuven, Belgium.

    Biography
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    Biography

    Matthias Schoenberger
    University of Leuven, Belgium.

    Matthias was born in Trier (Germany) and moved to Mainz University (Germany) for studying Biomedical Chemistry in 2004. During his study, he spend two research semesters at the Brookhaven National Laboratory (USA) and joined the laboratory of Joanna Fowler, pursuing research in radiochemical method development (11C) and in vivo positron emission tomography (PET) imaging. Matthias returned to Germany for his graduate studies in the International Max Planck Research School for Life Sciences (IMPRS-LS) in Munich with Dirk Trauner. His PhD-research was dedicated to organic chemistry, chemical biology and electrophysiology, yielding multiple first-in-classphotoswitchable ligands for ion channels and GPCRs. For his postdoctoral training, Matthias returned to the USA and the field of molecular imaging as a Marie-Slodowska-Curie fellow at Stanford University (Chemistry) and Harvard Medical School/MGH (Biomedical Imaging). Matthias’ group in the pharmacy departmentperformsf KUinterdisciplinaryLeuven (Belgium research in Chemical Biology and Imaging with the goal of understanding ion channel dynamics in human disease.



    Abstract
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    Abstract

    Matthias Schoenberger
    University of Leuven, Belgium.

    Voltage gated sodium channels constitute an integral component of electrical conduction in the heart and the nervous system. By depolarizing cardiomyocytes or neurons, they initiate action potentials that underlie the electro-mechanical coupling and neuronal firing. Changes in cardiac NaV function or expression levels can have severe consequences. For example, patients with loss-of-function or gain-of-function mutations in the SCN5A gene, which decodes cardiac NaV1.5, are at risk of suffering sudden cardiac death due to ventricular arrhythmias. However, it has not been possible to measure NaVs in vivo due to lacking molecular probes or techniques. To fill this gap and enhance our understanding of in vivo NaV function, we have developed the first positron emission tomography (PET) radiotracer for NaVs –radiocaine. In this talk, I will present the development and in vivo characterization of radiocaine and discuss steps towards future translational perspectives.

    Time:

    Title: Innovative Child Resistant Packaging for Pharmaceutical Solid Dosage Forms

    Lalji Baldaniya
    Anand Pharmacy College, India.

    Biography
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    Biography

    Lalji Baldaniya
    Anand Pharmacy College, India.

    Dr. Lalji Baldaniya is presently working as Assistant Professor at Anand Pharmacy College, Anand. He has 10 years of teaching and research experience. His area of research is formulation development of novel drug delivery systems, dissolution testing, PKPD study and innovation in pharmaceutical packaging systems. He has published more than 20 research papers in journals of national & international repute. He has also presented several papers in national & international conferences. He is recipient of the prestigious award from IPA ACG – SciTech Innovation 2016 in Solid dosage form development, and Pedagogical Innovation Award 2017. He has filed two patents. He involved in various research project sponsored by GUJCOST, BIRAC-SRISTI. He is reviewer of national & international journal in his research area of interest.



    Abstract
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    Abstract

    Lalji Baldaniya
    Anand Pharmacy College, India.

    The major oral solid medicines are sold in blister and strips packaging, which are packaged in a carton box. Children, who manage to open such boxes while unsupervised, are in great danger of being poisoned by swallowing the contents. A child resistant packaging (CRP) usually requires a special trick to open it. The aim was to design and validate performance of innovative child resistant packaging (CRP) system with unique features. Materials used were plastic grade, click lock system, and blister pack. The test was conducted on test panels of 100 healthy children between 42 to 51 months old were asked to open a pack. Each panel was divided into 3 groups, viz. 30 children of 42 to 44 months old, 40 children 45 to 48 months and 30 children 49 to 51 months. The panel of 100 healthy adults were divided into 3 groups, viz. 25 adults 18 to 40 years old, 25 adults 40 to 59 years old, and 50 adults 60 to 70 years old. Results showed only 6 % children succeeded to open CRP, while 94 % were failed to open CRP within 5 min. In case of adults, 96 % succeeded to open CRP without imposing any difficulties. There was a successful development of prototype of CRP, which can be used for the packaging of pharmaceutical products. Child resistant packaging deprived children to have access of medicated packaging system which resulted in devoid of any poisonous effect to the children.

    Sessions:
    Clinical pharmacy

    Time:

    Title: Optimizing Clinical Pharmacy Service Emergency Department

    Hussain Bakhsh
    King Abdulaziz University, Saudi Arabia.

    Biography
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    Biography

    Hussain Bakhsh
    King Abdulaziz University, Saudi Arabia.

    Dr. Hussain Bakhsh, Pharm.D., BCPS, is assistant professor at The Faculty of Pharmacy, King Abdulaziz University. Dr. Bakhsh earned his Doctor of Pharmacy degree from King Abdulaziz University after which time he was the first Saudi who completed Pharmacy Practice Residency and Emergency Medicine Specialty Residency training at The University of Arizona. Dr. Bakhsh completed a Postdoctoral Fellowship at The University of Arizona. His area of research includes: emergency medicine and toxicology. Dr. Bakhsh is a Board-Certified Pharmacotherapy Specialist. He is a member of the American Society of Health-System Pharmacist (ASHP) and the American College of Clinical Pharmacy (ACCP).



    Abstract
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    Abstract

    Hussain Bakhsh
    King Abdulaziz University, Saudi Arabia.

    The number of hospitals in the United States who reported the presence of emergency department (ED) clinical pharmacy services increased from 3.4% in 2006 to 16.4% in 2014 according to survey data. In 2011, the American Society of Health-System Pharmacists (ASHP) published Guidelines on Emergency Medicine Pharmacist Services. In Saudi Arabia, the provision of clinical pharmacy services in the ED is relatively new. Currently, ED pharmacy services in Saudi Arabia are limited to outpatient dispensing and medication history collection. A small number of Saudi EDs have recently started to assign pharmacists to provide limited direct patient care services such as (Patient endorsement in the ED, order verification, and code blue participation). Optimizing the clinical pharmacy services in the ED shows reduction of medication errors, improvement of therapeutic plans and facilitate cost saving.

    Time:

    Title: Impact of a pharmacist designed protocol for the management of acute alcohol withdrawal syndrome (AAWS) in the intensive care units of a community based health care system

    Roaa Khinkar
    King Abdulaziz University, Saudi Arabia.

    Biography
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    Biography

    Roaa Khinkar
    King Abdulaziz University, Saudi Arabia.

    Dr. Roaa M. Khinkar, PharmD, is a teaching assistant at College of Pharmacy, Department of Clinical Pharmacy, King Abdulaziz University. Dr. Khinkar graduated and earned her PharmD degree from King Abdulaziz University. Dr. Khinkar received King Abdulaziz University President’s Reward for achieving the highest GPA among medical candidates. Dr. Khinkar has completed her PGY1 Pharmacy Practice Residency at Hallmark Health System in Medford, Massachusetts. Currently, she is a PGY2 Quality and Safety Resident at Brigham and Women’s Hospital (BWH) in Boston, Massachusetts.



    Abstract
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    Abstract

    Roaa Khinkar
    King Abdulaziz University, Saudi Arabia.

    Background: Many cases of acute alcohol withdrawal syndromes are refractory to benzodiazepines. The demand for an appropriate protocol to manage such cases is on the rise. Previous studies done on phenobarbital have shown its superiority to benzodiazepines in terms of managing acute alcohol withdrawal syndromes. By other means, phenobarbital decreases the use of benzodiazepines, need for intubation, and ICU length of stay (ICU LOS). Objectives: This study was conducted to evaluate a new alcohol detoxification protocol using phenobarbital monotherapy as an alternative to benzodiazepines monotherapy. Methods: Design: Prospective cohort. Primary endpoint: Number of ventilation free days. Secondary endpoint: Total dose of benzodiazepines used, and ICU length of stay (ICU LOS). Inclusion criteria: Physicians utilized phenobarbital protocol when managing severe cases of alcohol detoxification resistant to benzodiazepines. Physicians evaluated if a patient is an appropriate candidate for the new alcohol detoxification protocol using protocol parameters and available patient data. Statistical analysis: For continuous data analysis, a two-sample t-test was used. Results: The number of ventilation free days was 6 days for pre-intervention group and 8 days for post-intervention group (P=0.27). The total dose of benzodiazepines used equivalent to lorazepam was 235 mg for pre-intervention group and 41 mg for post-intervention group (P=0.21). The total ICU length of stay (ICU LOS) was 6 days for pre-intervention group and 3 days for post-intervention group (P=0.05) Conclusion: The phenobarbital protocol has shown promise in terms of managing acute alcohol withdrawal syndromes even though the study did not have enough data to base conclusion.

    Time:

    Title: Evaluation of the antimicrobial effect of expired oral antibiotics In-Vitro

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy

    Biography
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    Biography

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy

    When human beings experience a disease or trauma, my great passion is bringing healing to them. I find myself in providing knowledge to others specially, my students to help sick people getting better. So keen in building a transparent relationship with my students’ because I believe that through them I will be able to achieve my goal in healing people. I know that no single approach is the right one for every individual, and so I trained myself to deal with each individually My educational background includes MSc in clinical pharmacy, University of Kabangsaan Malaysia (UKM)



    Abstract
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    Abstract

    Sasha Suliman
    Riyadh Colleges of Dentistry and Pharmacy

    In spite of significant risks and the non-clinical importance due to loss of potency, stiff penalties against administration of expired medications are still not appropriately enforced by health policy makers in developing countries [1] Objective: To investigate the effect of expiration on in vitro potency of oral antibiotic Methodology: Azithromycin, Clarithromycin, levofloxacin, cloxacillin, Tetracycline, Doxycycline; both control [un expired] and investigated [expired] antibiotic were compered based on the antimicrobial potentials by challenging local isolates of different concentration of S.aureus using agar diffusion technique Results: All Azithromycin, Clarithomycin, Levofloxacin and Cloxacillin showed significant difference (P- values: 0.005, 0.000, 0.000 and 0.001 respectively). The Difference in means of zone inhibition were 4.3mm ,7.3 mm ,8.3mm and 5.29mm respectively, which is still within the accepted efficacy range

  • Sessions:
    Drug Discovery and Development

    Time:

    Title: A fresh shine on cystic fibrosis inhalation therapy: antimicrobial synergy of polymyxin B in combination with silver nanoparticles.

    Raad Jasim
    Monash University, Australia.

    Biography
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    Biography

    Raad Jasim
    Monash University, Australia.

    Raad Jasim was born in Babylon, Iraq, in 1977. He received the bachelor degree in pharmaceutical sciences from Baghdad University, Iraq, in 2002. He worked as a pharmacist in the City of Medicine / Hospital of Special Surgeries, in Baghdad/ Iraq from December 2002 to October 2004. In November 2004 he joined the department of pharmacology and Therapeutics, Almustansria University/ College of Pharmacy as a demonstrator. In 2008 he received the Master degree in Pharmacology & Therapeutics from Kufa University/College of Medicine/Department of Pharmacology & Therapeutics, Iraq. In 2014 he has had started his PhD in the department of Drug Delivery, Disposition and Dynamics (D4), Monash Institute of Pharmaceutical Sciences, Monash University, Australia.



    Abstract
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    Abstract

    Raad Jasim
    Monash University, Australia.

    This in vitro study aimed to investigate the synergistic antibacterial activity of polymyxin B in combination with 2 nm silver nanoparticles (NPs) against Gram-negative pathogens commonly isolated from the cystic fibrosis (CF) lung. The in vitro synergistic activity of polymyxin B with silver NPs was assessed using the checkerboard assay against polymyxin-susceptible and polymyxin-resistant Pseudomonas aeruginosa isolates from the lungs of CF patients. The combination was also examined against the Gram-negative species Haemophilus influenzae, Burkholderia cepacia, Burkholderia pseudomallei, Stenotrophomonas maltophilia, Klebsiella pneumoniae and Acinetobacter baumannii that are less common in the CF lung. The killing kinetics of the polymyxin B-silver NPs combinations was assessed against P. aeruginosa by static time-kill assays over 24 h. Polymyxin B and silver NPs alone were not active against polymyxin-resistant (MIC ≥ 4 mg/L) P. aeruginosa. Whereas, the combination of a clinically-relevant concentration of polymyxin B (2 mg/L) with silver NPs (4 mg/L) successfully inhibited the growth of polymyxin-resistant P. aeruginosa isolates from CF patients as demonstrated by ≥2 log10 decrease in bacterial count (CFU/mL) after 24 h. Treatment of P. aeruginosa cells with the combination induced cytosolic GFP release and an increase of cellular reactive oxygen species. In the nitrocefin assay, the combination displayed a membrane permeabilizing activity superior to each of the drugs alone. The combination of polymyxin B and silver NPs displays excellent synergistic activity against highly polymyxin-resistant P. aeruginosa and is potentially of considerable clinical utility for the treatment of problematic CF lung infections.

    Time:

    Title: New discovery of treatment of complicated hemorrhoids without surgery

    Ahmed Msaad
    Taif University, Saudi Arabia.

    Biography
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    Biography

    Ahmed Msaad
    Taif University, Saudi Arabia.

    Dr Ahmed M. A. Masaad (PhD, MSc, BSc of Pharmacy). I concern with discovery of new drugs I have innovation in many new drugs and drug delivery system. I have memberships of many scientific society and editor of PSC journal. I published more than twenty scientific researches in many sides of pharmacy, I have discover new treatment of cancer of colon and skin I possess innovative certificates in drugs delivery system and new drugs which published in scientific journal.



    Abstract
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    Abstract

    Ahmed Msaad
    Taif University, Saudi Arabia.

    Hemorrhoids, also called piles, are vascular structures in the anal canal. In their normal state, they are cushions that help with stool control. They become a disease when swollen or inflamed; the unqualifiedly term "hemorrhoid" is often used to refer to the disease. The signs and symptoms of hemorrhoids depend on the type present. Internal hemorrhoids are usually present with painless, bright red rectal bleeding when defecating. External hemorrhoids often result in pain and swelling in the area of the anus. If bleeding occurs it is usually darker. The new treatment is mainly depend on mechanism of contract the connective tissue surrounding the venous around anus by effervescent tannin base with strong anti-bacterial, antifungal and anti-viral effect of formula. The safety of drugs was tested in rabbits, rats first and then the experiment was done in hundreds of patient under license of ethics committee of Taif University. The percentage of cure conducted was 99%, this success leads to relive of pain over millions of patients around the world and minimize the risk of surgery treatment and cost beside quick relive of disease in two to three weeks with no chance of relapse of disease again.

    Sessions:
    Protien-protien Interactions on Drug Targets

    Time:

    Title: Orally Bioavailable Antimalarial 4(1H)-Quinolone and 4(1H)-Quinolone Prodrugs with Single-Dose Cures

    RomanManetsch
    Northeastern University, USA.

    Biography
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    Biography

    RomanManetsch
    Northeastern University, USA.

    Dr. Roman Manetsch received his PhD in Chemistry in 2002 from the University of Basel under the guidance of Wolf-Dietrich Woggon. He joined the group of K. Barry Sharpless at the Scripps Research Institute working on click chemistry. In 2005, he moved to the University of South Florida and established a research group focusing on medicinal chemistry. In 2014, Dr. Manetsch assumed a position of an Associate Professor at Northeastern University. His current research focuses on lead discovery and optimization using synthetic chemistry in close conjunction with mass spectrometry.



    Abstract
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    Abstract

    RomanManetsch
    Northeastern University, USA.

    For approximately half a century, 4(1H)-quinolones such as endochin or ICI 56,780 were known to be causal prophylactic and potent erythrocytic stage agents in avian but not in mammalian malaria models. Hit-to-lead optimization of endochin lead to 4(1H)-quinolones ELQ-300 and P4Q-391, which target the liver, the blood as well as the transmitting stages of the parasite (Sci. Transl. Med. 2013, 5, 177ra37). However, poor aqueous solubility severely limits absorption and oral bioavailability and therefore impedes preclinical development of this class of antimalarials Herein, we disclose a general prodrug approach that converts promising lead compounds, such as antimalarial 4(1H)-quinolones, into aminoalkoxycarbonyloxymethyl (amino AOCOM) ethers that display significantly improved aqueous solubility and enhanced oral bioavailability. The prodrug is autarkic, independent of biotransformations, and animal-independent as it activates via a pH-triggered intramolecular cyclization reaction. Amino AOCOM ether prodrugs of antimalarial 4(1H)-quinolones were shown to possess pharmacokinetic and efficacy profiles significantly improved relative to the corresponding parent compounds (> 50-fold improvement of Cmax and AUC). One of the most promising 3-aryl-4(1H)-quinolone preclinical candidates was further shown to provide single-dose cures in a rodent malaria model at an unprecedentedly low oral dose of 3 mg/kg, without the use of an advanced formulation technique.

    Time:

    Title: Role of Impaired Aldehyde Detoxification in Aging and Parkinson Disease

    Elizabeth Fernandez
    University of Texas San Antonio, USA.

    Biography
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    Biography

    Elizabeth Fernandez
    University of Texas San Antonio, USA.

    I am currently Assistant Professor at the University of Texas Health Center and Research Health Scientist at the South Texas Veterans Healthcare System, San Antonio, Texas, USA. I am investigating the role of aldehyde detoxification in Parkinson’s disease. We developed colonies of mouse lines to examine the role of reduced expression of aldehyde dehydrogenase genes (Aldh1a1 and Aldh2), on pathology and behaviors associated with neurodegeneration of the nigrostriatal pathway. Our data indicates that reduced expression of each of these genes in the nigrostriatal exacerbate the behavioral and neurochemical deficits that are associated with increased expression of α-Syn.



    Abstract
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    Abstract

    Elizabeth Fernandez
    University of Texas San Antonio, USA.

    Parkinson’s disease (PD) is the second leading neurodegenerative disease affecting ~1% of people over the age of 65 years. Clinical features include, resting tremor, bradykinesia, and muscular rigidity. Pathologically, PD is characterized by the progressive loss of dopaminergic neurons in the nigrostriatal pathway, ultimately leading to dopamine deficits in the striatum. Surviving dopamine neurons have been found to contain cytoplasmic Lewy bodies primarily composed of the pre-synaptic protein, alpha-synulcein (aSyn). In its native form, aSyn remains unfolded. However, under certain conditions of oxidative stress, aSyn may aggregate and alter the release of neurotransmitters and the activity of mitochondrial complex I. Toxic aldehydes, including the dopamine metabolite, 3,4-dihydroxyphenylacetaldehyde, and the lipid peroxidation end product, 4-hydroxynonenal, promote aSyn aggregation. The two enzymes primarily responsible for the detoxification of aldehydes in dopamine neurons, are aldehyde dehydrogenase ALDH1a1 and ALDH2. Decreased expression of ALDH1a1 has been observed in the substantia nigra of patients who died with PD. Polymorphisms in ALDH2 have been found to increase the risk of PD in individuals exposed to pesticides. We previously reported that Aldh1a1-/-x Aldh2-/- knockout mice exhibit pathological manifestations of PD, including elevated biogenic aldehydes, motor deficits that are ameliorated by L-dopa, and the loss of TH-immunoreactive neurons. We tested the hypothesis that elevated biogenic aldehydes exacerbate the behavioral and neurochemical deficits that are associated with increased expression of α-Syn. The results show that elevated biogenic aldehydes, in the presence of overexpressed human wild-type aSyn, exacerbates neurochemical and behavioral deficits in the TTG mouse model of PD.

    Sessions:
    Novel Drug Delivery Systems

    Time:

    Title: In Vitro Permeation and Pharmacokinetic Evaluations of a Novel Transdermal Formulation of CZ48, Lactone-Stabilized Camptothecin-C20-propionate, for Cancer Treatment

    Yousif Rojeab
    Ohio Northern University, USA.

    Biography
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    Biography

    Yousif Rojeab
    Ohio Northern University, USA.

    Dr. Rojeab is currently an Associate Professor of Pharmaceutics at the Raabe College of Pharmacy, Ohio Northern University in Ada, OH, USA. He received his B.Sc. in Pharmacy in 1999 and Ph.D. in 2007 in Pharmaceutics from University of Houston, TX. He is also a licensed pharmacists in the states of OH, MI & TX. Current research in Dr. Rojeab’s lab involves two main areas. First is the physicochemical characterization, preformulation, formulation and delivery of anti-cancer agents. The second research area involves bioavailability/bioequivalence evaluations of novel and conventional orally administered solid dosage forms in man.



    Abstract
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    Abstract

    Yousif Rojeab
    Ohio Northern University, USA.

    Camptothecin (CPT) is a promising anti-cancer agent with high efficacy against a variety of solid tumors. The lactone ring of CPT is essential for the anti-tumor activity but is unstable in vivo. A pro-drug of CPT, CZ48, has been developed where CZ48 is enzymatically hydrolyzed in vivo to active CPT. For its anti-cancer activity, CPT levels need to be sustained and maintained over a period of time, which means the requirement for a continuous delivery of CZ48. It has been demonstrated that I.V. bolus injection of CZ48 provided higher concentrations of the active CPT compared to direct dosing of CPT at the same dosing level in Sprague-Dawley rats. Also, it was shown that CZ48 dosing resulted in sustained plasma levels of CPT for five hours in this animal model. Therefore, if we extrapolate these observations to man, we would expect to see this pattern of sustained CPT levels upon administration of the pro-drug, CZ48. This means delivery of CZ48 can achieve a continuous, therapeutically relevant drug concentration of CPT for anti-neoplastic indications. Transdermal delivery satisfies the requirement for continuous CZ48 delivery since it allows for predictable and controlled drug permeation through the skin. A promising approach to deliver drug molecules transdermally is through a microemulsion formulation. Our in vitro permeation data through excised rat skin as well as in vivo data upon topical application in Swiss-nude mice demonstrate the feasibility of achieving sustained plasma levels of CPT, required for anti-tumor activity, upon transdermal application of the pro-drug, CZ48.

    Time:

    Title: Scaffolds based on electrospun nanofibers for wound healing

    Giuseppina Sandri
    University of Pavia, Italy.

    Biography
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    Biography

    Giuseppina Sandri
    University of Pavia, Italy.

    Sandri Giuseppina (PhD in Chemistry and Pharmaceutical Technology) is Associate Professor at Drug Science Department (Faculty of Pharmacy) at University of Pavia, Italy. Current research concerns the development medical devices and antimicrobials based formulations based on electrospun nanofibers for skin and hearth regeneration. Her research activities were awarded with national and international awards and were founded with public and private grants. She takes part in joint research projects with Universities and pharmaceutical companies. She serves as reviewer for several scientific journals. Total number of referred publications: 86 contributions published on scientific journals, 12 book chapters, 3 patents; Hindex: 25 (Scopus).



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    Abstract

    Giuseppina Sandri
    University of Pavia, Italy.

    Electrospinning is a one-step and simple method to manufacture membranes based on (nano)fibers having diameters ranging from 20 nm up to 1m and more. Scaffolds based on nanofibrous membranes could allow protecting chronic skin lesion them from microbial contamination and optimal wound hydration and gas exchanges, crucial for healing, and should be a substrate able to induce cell adhesion and growth, speeding up the healing process. Given this premises the aim was the development of electrospun nanofibers based membranes as scaffolds to enhance cutaneous wound healing of chronic lesions and burns. The nanofibers were prepared starting from aqueous polymeric solutions to obtain insoluble membranes in aqueous fluids able to act as a support for cell growth, migration and proliferation. Nanofibers were loaded with either silver nanoparticles or norfloxacin, as antimicrobial agents. Chitosan and a glycosaminoglycan (hyaluronic acid or chondroitin sulfate) were selected as biomaterials. The developed scaffolds based on biopolymers are characterized by flexible area and shape with suitable elasticity and mechanical properties, are able to maintain optimal hydration and to absorb excess of fluids, to form barrier against microbial contaminations and to release antimicrobial agents. The presence of antimicrobials agents do not impair cell adhesion (fibroblasts and endothelial cells) and proliferation in an in vitro models. The in vivo evaluation in murine burn model suggests that scaffolds are effective to enhance wound closure without signs of inflammation and adverse effects.

    Time:

    Title: Development and efficacy evaluation of topical preparations for nail disorders

    Paola Perugini
    University of Pavia, Italy.

    Biography
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    Biography

    Paola Perugini
    University of Pavia, Italy.

    Prof. Perugini, graduated in Pharmaceutical Technology and Chemistry (CTF) and in Pharmacy, earned her PhD in Pharmaceutical Technology and Chemistry in 1998. Now she is an associate Professor at the University of Pavia in which she teaches in Pharmacy and in CTF degree courses. Furthermore she is the Coordination of the Master Degree in "Cosmetological Sciences" at the University of Pavia from 2009-2010. The main research activities of Prof. Perugini concerning pharmaceutical and cosmetic technology. The scientific work of Dr. Perugini has resulted in over 60 publications, 2 patents, more than 100 communications on topics of technology pharmaceutical and cosmetics.



    Abstract
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    Abstract

    Paola Perugini
    University of Pavia, Italy.

    This presentation proposes an overview of the development and testing of effective treatment for nail disorders, in particular onychodystrophy that is an alteration of the tropism of the nail, due to systemic and local causes. Topical treatment avoids adverse effects associated with systemic therapy. However, the effectiveness of topical therapies is limited because of the poor permeability of the lamina to the therapeutic agents applied. In fact, it must be borne in mind that the nail is basically a tortuous twist of crystalline and amorphous fibrous proteins in which only small molecules pass through. In recent years the research has mainly focused on improving transdermal permeability by chemical treatments, penetration enhancers, mechanical and physical methods. For this purpose, several "ex vivo" methods have been studied, including the use of bovine membranes as a human nail substitute for penetration studies. The aim of recent works was to evaluate whether and to what extent such in vitro tests can correctly predict the in vivo fate of nail lacquers. In the application of pharmaceutical lacquers used to treat nail alterations and fungal infections, the location of the film on the nail lamina and the transungueal passage of the active substance are the key factors. Hence the importance of developing topical products with appropriate formulation both in terms of medical treatment and associated cosmetic treatment. In fact, the constant use of an inappropriate cosmetic formulation can severely compromise the effectiveness of medical treatment.

    Time:

    Title: FORMULATION AND CHARACTERIZATION OF FLUCONAZOLE LOADED MCM-41 POWDER FOR TOPICAL DRUG DELIVERY

    Ankita Goswami
    saurashtra university, India.

    Biography
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    Biography

    Ankita Goswami
    saurashtra university, India.

    Myself, Ankita Goswami am currently pursuing PhD in pharmacy as a research scholar in Department of Pharmaceutical sciences, Saurashtra University, Rajkot, India. I have completed my Masters degree in pharmaceutics from the same institute in 2015 and Bachelors degree in Pharmacy in the year 2013 with distinction. I have been selected for a scholarship from the University grants commission of India called BSR Meritorious fellow for a period of 5 years during my PhD. Also, having passed the national level pharmacy aptitude test-GPAT in 2013, I had received a scholarship for 2 years for my Masters programme. I have 2 years of research and teaching experience till date. I have presented oral and poster presentations as well as served as a resource person in 10 national and 1 international conferences. As a researcher, I’m keenly interested developing patient compliant dosage forms. My areas of interest include, herbal anticancer formulations and formulation enhancement using nanotechnology.



    Abstract
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    Abstract

    Ankita Goswami
    saurashtra university, India.

    Background: The use of common carriers like talc for topical drug delivery leads to diminished efficacy as a result of poor aqueous solubility and low dissolution rate. Objective: The objective of this study was to improve the efficiency of Fluconazole topical dosage form using MCM-41 as a carrier material. Method: Fluconazole complex was prepared using MCM-41 and ß-CD as carriers in different proportions by melt, solvent evaporation and kneading method. The complex was converted into powder formulation. These formulations were subjected to anti-fungal activity tests. Results: The inclusion compound was characterized by X-ray powder diffraction (XRPD), FTIR and differential scanning calorimetry (DSC). The optimized method of preparation determined by in-vitro dissolution was melt method, and was later subjected to anti-fungal activity test. Out of all, formulation B containing MCM-41 as the bulk excipient had better performance; it showed 92.95±0.33% CDR at the end of 60 minutes and higher moisture adsorption. Conclusion: Thus, a Fluconazole topical formulation with improved drug dissolution and moisture adsorption was designed. From in-vitro tests, formulation B had better performances than the commercial formulation toward skin mycotic infections. Key Words: Fluconazole, mesoporous materials; skin mycotic infections; inclusion compound; X-ray diffractometry; drug dissolution; adsorption

    Time:

    Title: Brusatol Nanoparticles to Facilitate Clinical Translation for Chemotherapy

    Simeon K. Adesina
    Howard University, USA

    Biography
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    Biography

    Simeon K. Adesina
    Howard University, USA

    Dr. Simeon K. Adesina completed his Ph.D. at the Department of Pharmaceutical Sciences, Howard University. He has published several articles in peer reviewed journals and several abstracts and conference proceedings. He has a patent and another patent submission. He joined Howard University as Assistant Professor in 2012 and is currently an Associate Professor in the Department of Pharmaceutical Sciences, College of Pharmacy at Howard University.



    Abstract
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    Abstract

    Simeon K. Adesina
    Howard University, USA

    Brusatol has been reported to possess a unique potential to target two distinct pathways that are important in the progression of cancers by its specific action on nuclear factor erythroid 2-related factor 2 (Nrf2) and as a global protein synthesis inhibitor. Due to its non-selective mechanisms of action, it is unlikely that brusatol can be administered as a therapeutic agent without substantial host toxicity. The oil-in-water emulsification solvent diffusion method was modified to prepare brusatol-loaded, polyethylene glycol-coated polylactide-co-glycolide nanoparticles. Scanning electron microscopy revealed the formation of nanoparticles and average size was determined by dynamic light scattering. Drug content of the nanoparticle formulation was determined by high performance liquid chromatography. The in vitro release isotherm showed a biphasic and sustained release of the encapsulated drug. Toxicity of the brusatol-loaded nanoparticles in prostate cancer cell lines was evaluated over 120 hours using the Cell Titer 96® Non-Radioactive Cell Proliferation Assay. In vitro cytotoxicity data revealed that both brusatol-loaded nanoparticles and the control brusatol solutions at the same concentrations exhibited dose-dependent toxicity to PC-3 and LNCaP prostate cancer cell lines at the concentrations used in this study with the nanoparticle formulation showing more toxicity to both cell lines. Cellular uptake of nanoparticles was evaluated using confocal microscopy after a 6-hour incubation of rhodamine-123-loaded nanoparticles with PC-3 cells. Data showed internalization of the nanoparticles. This study shows that the nanoparticle formulation of brusatol can overcome delivery challenges and facilitate the clinical translation of the compound for the treatment of cancers.

    Time:

    Title: Fast Dissolving Drug Delivery Systems

    Gulay Yelken Demirel
    Yeditepe University, Turkey

    Biography
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    Biography

    Gulay Yelken Demirel
    Yeditepe University, Turkey

    Gulay Yelken Demirel has a degree in Department of Chemistry from University of Gazi (Ankara, Turkey) followed by a master degree in Medicinal and Pharmaceutical Chemistry (faculty of pharmacy) from the same university and studied Executive Master of Business Administration at Istanbul University. Currently, She has an ongoing PhD in Medicinal and Pharmaceutical Chemistry(faculty of pharmacy) at Yeditepe University. She is also a Turkish Patent Attorney. She has eleven years experience in R&D department of generic pharmaceutical companies. She worked as formulation scientist at the Pharmaceutical Technology Department of Nobel Pharmaceuticals. Presently, She owns the R&D Project Group Executive position at Sanovel Pharmaceuticals. She has several published papers in the academic areas and over 100 patents&patent applications in the industrial areas on pharmaceutical dosage forms.



    Abstract
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    Abstract

    Gulay Yelken Demirel
    Yeditepe University, Turkey

    Fast-dissolving formulations represent excellent opportunities for life cycle management to the pharmaceutical companies. Fast dissolving technologies have many advantages like ease of swallowing, administration without water, quick onset of action for improving both patient convenience and compliance as benefits for the patient; extended life cycle, product differentiation, patent protection as benefits for pharmaceutical companies... But there are some challenges for formulation development studies like taste -masking, disintegration time, moisture sensitivity, friability, packaging and intellectual property issues, especially for the generic companies. The technologies are under patent protection like Zydis ® , Flashtab®, OraSolv® and DuraSolv™, WOWTAB®.... One of the major issues is a taste-masking problem may be overcome with using cyclodextrins, polymer coating, flavoring&sweetening agent, microencapsulation techniques... There are some modified excipients for providing both taste-masking and productability properties in the formulation like Ludiflash®, Pharmaburst®... From the analytical development point of view, there are a number of different methods from conventional dosage forms which are determined in the Pharmacopoeias. And for comparison and assessment of taste masking, electronic tongue may be a good opportunity which was developed by Alpha M.O.S. In the sense of generic companies, developing a fast dissolving tablets version of an existing immediate-release product means that the two formulations must be bioequivalent and this can be challenging for in-vivo studies especially if the method of taste masking retards the dissolution rate of the active ingredient after disintegration. What about the future of fast dissolving technologies? Orally disintegrating extended Release (ODT-ER) dosage forms are providing all of the benefits of these two drug delivery technologies in a single pharmaceutical product. And oral rapid films also may be a good alternative, especially for the OTC market.

    Sessions:
    Biopharmaceutics and Pharmaceutics

    Time:

    Title: Mechanisms of Drug-Herb Interactions and Clinical Implications

    Arkene Levy
    Nova Southeastern University, USA.

    Biography
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    Biography

    Arkene Levy
    Nova Southeastern University, USA.

    Dr. Arkene Levy is an Associate professor of Pharmacology and Pharmacognosy at the Nova Southeastern University in Florida USA. Dr. Levy completed her PhD in Pharmacology at the University of the West Indies in Jamaica, and postdoctoral studies as a Fulbright Scholar at Moore’s Cancer Centre, UCSD California. Dr. Levy has extensive research experience on the effects of natural products in cancer and inflammatory disease models. Dr. Levy is an editorial consultant for the Current Topics in Nutraceutical Research journal and a member of both the American Society for Pharmacology and Experimental Therapeutics and the American Association for Cancer Research.



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    Abstract

    Arkene Levy
    Nova Southeastern University, USA.

    The clinical evidence for interactions between conventional drugs and herbal medicines is steadily increasing globally. This can be attributed to many factors including increasing trends towards consumption of herbal supplements for various health benefits, loose regulation of the herbal drug industry, and the need for proper training of healthcare professionals on the potential hazards of herbal supplements. Herbs contain potent chemical constituents that can affect both the pharmacodynamic and pharmacokinetic profiles of conventional drugs when simultaneously administered. The mechanistic basis for these interactions include antagonism or potentiation of therapeutic effects, and altered absorption, metabolism or elimination of drugs. Many herbs have been implicated in these regards. Garlic (Allium sativum) has been shown to increase risk for bleeding in patients on warfarin; Licorice (Glycyrrhiza glabra) antagonizes the effects of anti-hypertensives; Green tea (Camellia sinensis) impairs the absorption of nadolol; and St John’s Wort (Hypericum perforatum) induces the metabolism of cyclosporine and numerous other drugs that are dependent of the cytochrome P450 enzymes for biotransformation. Based on these lines of evidence, there is a still a greater need for education of health care providers and patients about the risks of herbal medicines and for integration of evidenced-based herbal medicine knowledge into curriculums of medical schools globally . Additionally, further clinical research and pharmacovigilance is needed to gather more evidence on the incidence of drug-herb interactions and patient outcomes in this unique setting. This data would have significant implications towards guiding the development of stricter regulations for the herbal drug industry.

    Time:

    Title: How can the chemical-physical properties of the solution influence the formation and the morphology of amyloid proteins?

    Carlotta Marasini
    University of Copenhagen, Denmark.

    Biography
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    Biography

    Carlotta Marasini
    University of Copenhagen, Denmark.

    I am a physicist with a specialization in biophysics. My specialization focuses on the study of different proteins involved in several diseases combining biophysical and biochemical techniques. During my master, I spent six months at the ILL in Grenoble studying the aggregation of insulin by neutron scattering. I received my Ph.D. in bioengineering from the University of Genoa in 2013, performing a structural study of a protein responsible for Cystic Fibrosis disease. Since then, I have worked as postdoc at the University of Copenhagen, where I am studying analysis the structure and kinetics of different proteins involved in neurodegenerative diseases.



    Abstract
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    Abstract

    Carlotta Marasini
    University of Copenhagen, Denmark.

    Protein amyloid-like aggregation is related to devastating pathologies as Alzheimer´s and Parkinson´s diseases. During this process, the protein native state converts into a partially unfolded non-native state and then associates into smaller, soluble oligomers of different sizes. Subsequently, elongated fibrils are formed (i.e. fibrillation). In some protein system, also amyloids-like superstructures (spherulites) have also been found in vitro during the aggregation (1,2) and in vivo (3,4). Importantly, monomers, oligomers, fibrils, and spherulites coexist in an equilibrium that is influenced by the environment (5,6). It is known that water, co-solutes, small molecules, concentrations, temperature, etc., play a crucial role in the native state of proteins, changing the structure and influencing inter-protein interactions. In this study, we aim to demonstrate how the chemical-physical composition of the solutions as well as the temperature, not only affect the protein initial states but also the dynamic of the fibrillation, the equilibrium between the species formed and the morphology of the mature fibrils (7). Also, our results emphasize that the final state of the fibrillation is not a stationary state but still a dynamic system that also after have been formed it can be highly influenced by incubation at different temperatures, pH and times. 1. PNAS. 2004, 101,14420–14424. 2. Biointerfaces. 2012, 89, 216– 222. 3. J Alzheimers Dis. 2010, 20, 1159–1165. 4. Vet Pathol. 2000, 37, 104–107. 5. Biophys J. 2010, 99, 3801-10. 6. Nature communications. 2013, 4, 1891. 7. RSC Adv. 2017, 17, 10487-10493.

    Time:

    Title: Therapeutic drug plasma monitoring of antimicrobials focusing PK/PD approach in critically ill septic patients

    Silvia Regina Cavani Jorge Santos
    University of Sao Paulo, Brazil.

    Biography
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    Biography

    Silvia Regina Cavani Jorge Santos
    University of Sao Paulo, Brazil.

    Professor Silvia Regina Cavani Jorge Santos University of São Paulo, SP, Brazil • Academic life: School of Pharmaceutical Sciences, University of Sao Paulo (1988-2018). Chair - Clinical Pharmacokinetics, School of Pharmaceutical Sciences, University of Sao Paulo since 2001, Lecturer/undergraduate and graduated students: disciplines Pharmacotherapy – Pharmacokinetics (basic, applied, clinical and advanced). • Academical studies: University of Sao Paulo Pharmacist (1972), MS.Pharm. (1976) doctoral sandwich grant UK, Bradford University, Pharm. D. (1982). Post doctoral studies in Medical Schools, Essen Universitat (1986) Germany, Kiel Universitat (1987) Germany, LACDR – Leiden University (1994) The Netherlands. • Research Group Lieder "National Council of Brazilian Research" Clinical Pharmacokinetics Group", 1990-2018.



    Abstract
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    Abstract

    Silvia Regina Cavani Jorge Santos
    University of Sao Paulo, Brazil.

    Mortality and morbidity associated with sepsis remain unacceptably high. Detection of patients in their early stages of the infection is crucial to diminish the high mortality rate associated with sepsis. Further studies are warranted to determine the optimal targets for resuscitation and the best antimicrobial management especially when MDR pathogens are involved. On the other hand, the hospital acquired pneumonia (HAP) is one of the most common infections acquired among hospitalized patients. associated with increased mortality and high hospital costs. The rise in the ICU‑ acquired infections and ventilator-associated pneumonia (VAP) remains the most common infection in ICU due to antibiotic-resistant bacteria also causes an increase in the incidence of inappropriate empirical antibiotic therapy, with an associated increased risk of hospital mortality. It is very important to know the most common pathogens responsible for these infections in each hospital and each Intensive Care Unit, and the antimicrobial susceptibility patterns of isolated strains, in order to reduce the incidence of inappropriate antibiotic therapy and improve the prognosis of patients. Metabolic and pharmacokinetics changes are expected in critically ill ICU-patients adults or pediatrics in septic shock. In general, renal insufficiency occurs as a consequence of hypovolemia during the systemic inflammatory response syndrome (SIRS); then, the reduction of daily dose by 50% is recommended in these patients to avoid adverse effects and toxicity. Changes in pharmacokinetics result in antimicrobials (AM) plasma concentration lower than those expected and therapeutic fail occurs after the empiric dose regimen in ICU-patients. The control of nosocomial infection is a challenge, once antimicrobial effectiveness depends on drug levels during the antimicrobial therapy that must start as soon as possible after patient admission in ICU at the empiric dose regimen recommended. Therapeutic drug plasma monitoring (TDM) applied to vancomycin and -lactams agents as piperacillin, meropenem and imipenem plasma measurements is quite useful combined to pharmacokinetic-pharmacodynamic approach based on several predictor index recommended to those antimicrobials to support the necessity of dose adjustment by the Clinical Team. Biomarkers-TDM-PK/PD approach guided antimicrobial therapy is the new pack of tools available to the ICU-Clinical Team to guarantee drug effectiveness. Finally, these tools must be applied to appropriate monotherapy or combination of two agents focusing the cure of infection by eradication of nosocomial strains isolated from hemocultures of each ICU-patient.

    Sessions:
    Nutraceuticals

    Time:

    Title: Specific Omega-3, Omega-6 Polyunsaturated Fatty Acids and γ-Tocopherol in the Therapy of Relapsing Multiple Sclerosis (MS): the Paradigm of NEUROASPIS PLP10 Intervention Efficacy

    Ioannis Patrikios
    European University Cyprus, Europe.

    Biography
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    Biography

    Ioannis Patrikios
    European University Cyprus, Europe.

    Professor Ioannis S. Patrikios completed his PhD studies in Biochemistry in 1994, from the City University of New York, USA. Recently he was offered and now serves as a Fellow of the Academy of Forensic Medical Science, UK and member of the Advisory Board. His 2002 research findings on the effect of frying oils as human hemagglutinins got an international interest. He has been appointed as a reviewer of international scientific journals in the field of Medicine, Med-Biochemistry, Pharmaceuticals-Therapeutics and Neurology and as a reviewer for research grants. Professor Patrikios is the founder and solid organizer of the annual event “International Multithematic Medical Congress, Biomedical Scientific Cyprus (BSC)” an International annual scientifically prestigious congress. He is a member of several International associations and bodies including Sigma Xi. He is the chief scientific investigator of the team that lately invented and patented the nutraceutical formula Neuroaspis® PLP10 as a new therapeutic intervention for multiple sclerosis. At present, he serves as the Acting Chairman of the School of Medicine, European University Cyprus and affiliated as a research collaborator at the Cyprus Institute of Neurology and Genetics, Cyprus.



    Abstract
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    Abstract

    Ioannis Patrikios
    European University Cyprus, Europe.

    Introduction: MS treatments are products of reductionism, partially effective with no (re)myelinating/neuroprotective abilities associated with significant side-effects. We aimed to assess whether our novel interventions, formulated based on systems medicine (SM), comprising specific polyunsaturated fatty acids (PUFA) and vitamins reduce disease activity in patients with relapsing remitting (RR) MS who were either treated with disease modifying treatment (DMT) or untreated. Methods: We contacted a 30-month randomized, double-blind, placebo-controlled, proof-of-concept clinical study at the CING. Of a total of 80 patients, 20 were randomly assigned to receive intervention A (docosahexaenoic acid (DHA)/eicosapentaenoic acid (EPA) (3:1w/w) omega-3, linoleic acid (LA)/gamma(g)-linolenic acid (GLA) (2:1w/w) omega-6 fatty acids, omega-3/omega-6 (1:1w/w), other specific PUFA, monounsaturated fatty acids (MUFA), minor quantity of specific saturated fatty acids (SFA), vitamin A and vitamin E), 20 to receive γ-tocopherol, intervention C, 20 to receive the combination of A and C, intervention B (PLP10) and 20 to receive placebo, as an oral solution, once daily. The primary end point was the annualized relapse rate (ARR) and the key secondary end point was the time to disability progression. ISRCTN87818535. Results: PLP10 reduced the ARR by 70% (p=0.003), in relation to the baseline ARR and the placebo increased by 46% (p=0.354). For the primary end point, PLP10 reduced the ARR by 58% (p=0.016) and for the secondary end point, significantly reduced the risk of sustained progression of disability by 86% over the 2-year period (p=0.047) vs. placebo. More patients in the PLP10 group (72%) vs. placebo group (20%) were free from new or enlarging T2-weighted lesions on brain MRI scans over the 2-year study. No adverse events were reported. Interventions A and C showed no significant efficacy. Discussion: PLP10 treatment significantly reduced the ARR, and the risk of sustained disability progression without any adverse or significant side effects. This is the first clinical study of SM approach medical nutrient formula that holds strong promise as an effective treatment for RRMS.

    Time:

    Title: The effect of nutrition program on health status among dialysis patients

    Mazen El-Sakka
    Al Azhar University-Gaza, Egypt.

    Biography
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    Biography

    Mazen El-Sakka
    Al Azhar University-Gaza, Egypt.

    o PHD in Pharmacy – Pharmacognosy o Clinical Nutritionist Consultant o 3 Books & 10 Academic courses o 3 Patents o More than 30 publications o More than 55 international congress & workshop participations



    Abstract
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    Abstract

    Mazen El-Sakka
    Al Azhar University-Gaza, Egypt.

    Poor access to food among low-income adults has been recognized as a risk factor for chronic kidney disease (CKD), but there are no data for the impact of food insecurity on progression to end-stage renal disease. Diet and anthropometrics measurements are associated with increased morbidity and mortality and a rapid deterioration of kidney function in patients with chronic kidney disease. However, there is little information regarding the effect of nutrition intervention. The aims of this study were to investigate whether of diet patterns and changes in biochemical parameters as well as eGFR among adults with CKD on dialysis, as well as, to evaluate the efficacy and safety of a nutrition education program in patients with dialysis, based on the diagnostic criteria for Protein–energy wasting. The design of the study was a 2-month Control experimental design, prospective, and interventional study. The study was started from May 2017 and expected to finish on December 2011 in the Dialysis Department of Al Shifa Hospital in Gaza, Palestine. Subjects A total of 133 patients with ESRD started the research study, 102 finished it and 51 subjected on very well controlled diet program. Intervention The 2-month nutrition education program consisted of designing an individualized diet plan based on the patient's initial nutritional status, and 4 nutrition education sessions. Results and Conclusion Promising results reveal the effect of diet program on anthropometric, biochemical, and eGFR. Further research is needed to investigate the reliability and utility of this tool in a larger population group.

    Time:

    Title: Anti-cancer activity of the phytochemical Indicaxanthin: in vitro and in vivo studies against melanoma

    Alessandro Attanzio
    University of Palermo, Italy.

    Biography
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    Biography

    Alessandro Attanzio
    University of Palermo, Italy.

    Researcher - BIO/10 Biochemistry PhD in Pharmaceutical Sciences Graduate degree in Biomedicine with a vote of 110/110 and praise Patent Inventor Guest Editor - Journal of Food Quality- Author of 25 publications on international biochemical and nutraceutical journals



    Abstract
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    Abstract

    Alessandro Attanzio
    University of Palermo, Italy.

    Epidemiologic studies show promising results supporting the role of natural compounds in the chemoprevention of melanoma. Indicaxanthin is a betalain pigment from cactus pear fruit, capable of modulating specific redox-driven pathways involved in the inflammatory reaction in vitro (1). Interestingly, indicaxanthin is bioavailable and exerts strong anti-inflammatory effects when orally administered at nutritionally-relevant doses in rats (2). A causative link between inflammation and melanoma has recently been explored. In line with this, we investigated the antitumor potential of indicaxanthin vs melanoma both in vitro and in in vivo mouse model. Indicaxanthin inhibited proliferation of human A375 melanoma cells. The inhibition measured at 24h was concentration-dependent in the range between 50 and 200 μM, with a maximum of 52% at the highest concentration. Indicaxanthin induced cell apoptosis as cytofluorimetrically revealed by double AnnexinV/PI staining. Moreover indicaxanthin time-dependently inhibited the activation of NF-kB, a transcriptional factor conferring tumor survival capacity and escape from apoptosis. In addition, the expression of Bcl-2 and c-FLIP, two inhibitors of apoptosis the expression of which is modulated by NF-kB, was decreased. More importantly, indicaxanthin (3.2 mg/kg) orally-administered for 15 days to mice when the injected tumor had reached an average 3-4 mm diameter, induced a reduction of tumor volume (86%) and weight (83%). 1. Allegra, M et al. (2014) Redox Biol 2:892-900 2. Allegra M et al. (2014) J Nutr 144:185-92

  • Sessions:
    Pharmaceutical Management

    Time:

    Title: Medical Specialty Management is Key to Managing Drug Spend and Trend

    Saira Jan
    Rutgers State University of New Jersey, USA.

    Biography
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    Biography

    Saira Jan
    Rutgers State University of New Jersey, USA.

    Dr. Jan is the clinical professor at Ernest Mario School of pharmacy at Rutgers University of NJ and leads the Horizon Blue Cross Blue Shield of New Jersey pharmacy clinical management program as a Clinical Director with over 19 years of experience in health care management and research and academics. She has implemented many clinical programs such as Opioid management, Rheumatoid arthritis, Star measures, MTM, adherence and childhood obesity initiatives in the state of New Jersey in addition to serving as a consultant to many industry foundations and committees. Dr. Jan has a Masters in Pharmacology from St John’s University and a doctorate in Pharmacy from Rutgers State University of New Jersey.



    Abstract
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    Abstract

    Saira Jan
    Rutgers State University of New Jersey, USA.

    In 2015, approximately half of all specialty drug spend was billed under the medical benefit.1 In that same year, the FDA approved a record-setting 45 novel medications, including 6 biologic agents, besting its ten year average approval rate of 28 novel drugs per year.2 In 2016, the FDA approved 13 new drugs that fell under the medical benefit. These approvals included four drugs in the oncology space, three drugs for bleeding disorders, and two for rare pediatric neuromuscular disorders. With the flood of specialty biologics to the market, drugs billed to the medical benefit continue to be cost drivers for the overall drug trend. Since 2011, drug spend on the medical benefit has increased by 55%.3 The overall per member per month spend for commercial members for medical benefit drugs was $23.68 in 2015, with oncology agents making up approximately half of that spend.3 Other specialty categories such at biologic drugs for autoimmune disorders, immune globulins, antihemophilic factor products, multiple sclerosis, and rare and orphan disease agents also make up a large chunk of the drug spend on the medical benefit, representing about 76% of the total medical drug spend in 2015.3 Because of these factors, effective management of specialty drugs on the medical benefit is critical to controlling drug spend and trend. A variety of strategies can be used to manage specialty spend on the medical benefit including appropriate utilization management through prior authorization and claim editing, site of care management, and reimbursement strategies that drive utilization towards generics and low cost alternatives. Identification of high cost members and outlier management can be another crucial component to effectively management cost. Overall, effective management strategies can yield a 10%-20% savings on medical benefit drug spend.

    Time:

    Title: Study of learning directions changes in Pharmacy specialization

    Meliksetyan Aghasi Armenakovich
    Saint-Petersburg Chemical-Pharmaceutical Academy, Russia.

    Biography
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    Biography

    Meliksetyan Aghasi Armenakovich
    Saint-Petersburg Chemical-Pharmaceutical Academy, Russia.

    Meliksetyan Agasi – lecturer in Saint-Petersburg Chemical-Pharmaceutical Academy, Russia. Ph.D in Pharmacy. Date of Birth: 03 March 1991. Residency: Saint-Petersburg, Russia.



    Abstract
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    Abstract

    Meliksetyan Aghasi Armenakovich
    Saint-Petersburg Chemical-Pharmaceutical Academy, Russia.

    The article represents an analysis of change in the structure of educational programs in the discipline "Pharmacy" based on example of Saint-Petersburg Chemical-Pharmaceutical Academy (SPCPA). The main historical phases of development of higher pharmaceutical education in Saint-Petersburg Chemical-Pharmaceutical Academy are also considered.

    Sessions:
    Oral Pharmacognosy & Phytochemistry

    Time:

    Title: Anti-Alopecia activity of Hantap (Serculia coccinea Jack.) leaves ethanol extract

    Resmi Mustarichie
    Padjadjaran University, Indonesia.

    Biography
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    Biography

    Resmi Mustarichie
    Padjadjaran University, Indonesia.

    Resmi Mustarichie (male) is Professor of Pharmaceutical Chemistry . He was graduated as PhD. Graduate supervised by Prof. Allan F.M Barton from Murdoch University, Western Australia. He is currently active as lecturer and researcher at Faculty of Pharmacy, Universitas Padjadjaran, Indonesia, 45363. His interest in Analysis and determination of bioactive compounds from natural materials, Herbal anti-alopecia, and molecular computing



    Abstract
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    Abstract

    Resmi Mustarichie
    Padjadjaran University, Indonesia.

    Objective: This study aims to determine and verify the use of ethanol extract of Sterculia coccinea leaves ethanol extract as stimulant of hair growth or anti-alopecia. Methods: The S. coccinea leaves was collected from Salawu tribe, Tasikmalaya, West Java. The extraction was done by maceration which was based on standard method of Indonesian Herbal Pharmacopeae. The viscous concentrated extract was fractionated by liquid-liquid extraction. Phytochemical screening according to the Farnsworth method. Method of hair growth on Angora type rabbit was modified of Tanaka method. Results:The results of phytochemical screening using Farnsworth method showed the ethanol extract containing secondary metabolite of tannin compound, polyphenol, steroid, triterpenoid, quinone, monoterpenoid and sesquiterpenoid. The results of hair fertilizer testing using Tanaka method showed that ethanol extract and water extract with 20%, 15%, 10% concentration significantly could fertilize hair with test for 18 days. Extracts of ethanol with levels greater than 10% showed better results. Water fraction of 10% appeared to show the best result for rabbit hair growth overcome minoxidil. Conclusions: This work found that the concentration of 10% ethanol extract of S. coccinea and its water fractions were affective for hair growth on male rabbits. It was suggested, however, for further study to determine the compound which was responsible by using elucidation methods and being tested to volunteers. Keywords: Hair fertilization, ethanol extract, Sterculia coccinea, hair grower, Alopecia, phytochemical screening

    Time:

    Title: Antigenotoxic Properties of Paliurus Spina Mil Fruits and their Active Compounds

    Murat Zor
    Bülent Ecevit University, Turkey

    Biography
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    Biography

    Murat Zor
    Bülent Ecevit University, Turkey

    I was born in Ankara-Turkey. Completed my primary,secondary and high school education in Ankara. I receieved my BS degree from Hacettepe University Faculty of Pharmacy in 1985. I got my BA and phD degree from Hacettepe University Institute of Health Sciences Pharmacognosy program in 1989. I am the founding member of faculty of pharmacy at Bülent Ecevit University (BEUN). I have been teaching both theoretical and laboratory courses. Currently, I am the head of Department of Pharmacognosy at Faculty of Pharmacy, and the director of professional Sciences at BEUN. My research interests include the isolation and structural elucidation of natural products which can be leading compounds in the drug discovery studies .



    Abstract
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    Abstract

    Murat Zor
    Bülent Ecevit University, Turkey

    Paliurus spina-christi Mill. (PS) fruits are widely used for different medical purposes in Turkey. Like in many medicinal herbs the studies concerning their activity, the activities of PS are also not well clarified. The aim of this study is to evaluate the antigenotoxicity of the compounds isolated and identified from the extracts of PS fruits. PS fruits were collected from Kastamonu region of Turkey and kept in air-dried room temperature (20 ± 2 °C). Air dried fruits (300 g) were powdered using a mechanical grinder and extracted with 1000 mL of MeOH:H2O (70:30 v/v) in 40 °C in a continuous extractor for 48 h. The extract was then concentrated at 40 °C using a rotary evaporator (Büchi RE 111, Büchi EL 131, Flawil, Switzerland) and used for the phytochemical investigations. The extract was pre-fractionated with n-hexane, EA, and n-butanol, respectively. The active compounds were separated, isolated, and determined by chromatographic methods and their structural elucidation was performed by Nuclear Magnetic Resonance (NMR) methods. The isolated major compounds were identified as (+/−) catechins and gallocatechin from EA fraction and rutin from n-butanol fraction of PS fruits. Their chemical structures were identified by 1H-NMR, 13C-NMR, HMBC, and HMQC techniques. It has been suggested that PS fruits and their compounds catechins, gallocatechin and rutin may have beneficial effects in oxidative DNA damage. It seems that PS fruits may be used in protection of the disorders related to DNA damage

    Sessions:
    Pharmacy Practice

    Time:

    Title: Impact of Diabetes Care by Pharmacists as Part of Health Care Team in Ambulatory Settings: A Systematic Review and Meta-analysis

    Alaa Bagalagel
    King Abdulaziz University, Saudi Arabia.

    Biography
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    Biography

    Alaa Bagalagel
    King Abdulaziz University, Saudi Arabia.

    Dr. Alaa Bagalagel, Pharm.D., CDE, is assistant professor of clinical pharmacy at the Faculty of Pharmacy, King Abdulaziz University. Dr. Bagalagel earned his Doctor of Pharmacy degree from King Abdulaziz University after which time he completed Pharmacy Practice Residency and Ambulatory Care Specialty Residency training at The University of Arizona. Dr. Bagalagel completed a Postdoctoral Fellowship at The University of Arizona. His area of research includes: clinical safety and efficacy of biosimilars, diabetes care, and drug reactions. Dr. Bagalagel is Certified Diabetes Educator. Dr. Bagalagel provides a service as Ambulatory care consultant at King Abdulaziz University Hospital. He is an active member of the biosimilar working group at The University of Arizona Health Outcomes and PharmacoEconomic Center.



    Abstract
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    Abstract

    Alaa Bagalagel
    King Abdulaziz University, Saudi Arabia.

    Objective: A comprehensive systematic review and meta-analyses examining the impact of pharmacist interventions as part of health care teams on diabetes therapeutic outcomes in ambulatory care settings. Data Sources: PubMed/MEDLINE, EMBASE, Cochrane Library, International Pharmaceutical Abstracts, Web of Science, Scopus, WHO’s Global Health Library, ClinicalTrials.gov, and Google Scholar were searched (1995 to February 2017). Search terms included pharmacist, team, and diabetes. Study Selection: Full-text articles published in English with comparative designs, including randomized controlled trials, nonrandomized controlled trials, and pretest-posttest studies evaluating hemoglobin A1C (A1C), were assessed. Data Extraction and Synthesis: Two reviewers independently screened for study inclusion and extracted data. Quality of the studies was assessed using tools developed based on the framework of the Cochrane Collaboration’s recommendations. Data Synthesis: A total of 1908 studies were identified from the literature and reference searches; 42 studies were included in the systematic review (n = 10 860) and 35 in the meta-analyses (n = 7417). Mean age ranged from 42 to 73 years, and 8% to 100% were male. The overall standardized mean difference (SMD) for A1C for pharmacist care versus comparison was 0.57 (P < 0.01), a moderate effect representing a mean difference of 1.1% (95% CI = 0.88-1.27). The effects for systolic blood pressure and low-density lipoprotein cholesterol were between small and moderate (SMD = 0.31 and 0.32; P < 0.01). The heterogeneity was high for all outcomes (>83%), indicating functional differences among the studies. No publication bias was detected. Conclusion: Pharmacists’ interventions as part of the patient’s health care team improved diabetes therapeutic outcomes, substantiating the important role of pharmacists in team-based diabetes management.

    Time:

    Title: Strategic Role & Interdisciplinary Cooperation of Pharmacists in the Optimisation of Pharmacotherapy in Ageing Population

    Nitish Chauhan
    Kiet School of Pharmacy, India

    Biography
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    Biography

    Nitish Chauhan
    Kiet School of Pharmacy, India

    Mr Nitesh Chauhan is working in the capacity of an associate professor in KIET School of Pharmacy, KIET, Ghaziabad. He is having over 10 years of experience in teaching, academics, learning and training. He is a certified corporate trainer by McGraw-Hill Education. He is also a well known consultant for pharmaceutical industry and guiding, inspiring and motivating many individuals in the very demanding field of entrepreneurship. Mr Nitesh Chauhan is an editor of one of the renowned International Journal which is published by Imed Publications, USA. Mr Nitesh Chauhan represented Indian pharmacy & scientific community at various International Forum like FIP, Switzerland, London UK, UAE etc. He has many scientific papers to his credit.



    Abstract
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    Abstract

    Nitish Chauhan
    Kiet School of Pharmacy, India

    One of the world's striking trends that have been widely recognized is the ageing of its population, which is constantly increasing. The pharmaceutical care of the elderly population is of utmost concern in the westernized countries. Even in India, where the average life expectancy is 62 years, the ageing population is growing. The number of elderly women at any point of time is reported to be more than the number of elderly men. As reported with time there will be around 20% increment growth of elderly population, which is huge percentage as ACGR is concern. The world's elderly population people 60 years of age and older - is 650 million. By 2050, the “ageing" population is forecast to reach 2 billion. With the advancement of science and medicine, life span and productive life of an individual has greatly increased. Added to this is the advances made by the pharma industry in providing affordable modern drugs to control the different diseases. All these factors have contributed to the rise in the number and percentage of elderly population all over the world and even in India. Elderly are more likely to experience drug related problems than any other age groups and also the biggest prescription users (40%) than any other group in the community. Hence, it is necessary and essential to elucidate the role of community pharmacist in the pharmaceutical care of elderly. Community pharmacist should possess certain basic skills, relearn basic pharmacy practice skills, apply the skills for elderly care and carry out the preventive care, and also develop a rational and feasible pharmaceutical care plan (PCP) for the elderly care. As such, community pharmacists therefore can implement quite a few aspects of PCP in India and give benefit of their knowledge to the patients at large and to maximise the functional ability of elderly population.

    Sessions:
    Poster Presentations

    Time:

    Title: Comparing The Pharmacological Activity Of Moringa Oliefera Lam(Moringaceae) Seed With The Leave In Reduction Of Blood Metal Load Using Albino Rats

    Patience Ugwu
    University of Nigeria, Nigeria

    Biography
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    Biography

    Patience Ugwu
    University of Nigeria, Nigeria

    Pharm Patience is a pharmacist that currently lectures at University of Nigeria Nsukka under the Faculty of Pharmaceutical Sciences, Department of Pharmacognosy and Environmental Medicine. Her research areas includes medicinal plants and Environmental pollutants, Phythochemistry and Natural products. Have OND in computer education (2001), B. pharm in pharmacy (2008) and M. Pharm in Environmental medicine in the year 2015, all from University of Nigeria Nsukka.



    Abstract
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    Abstract

    Patience Ugwu
    University of Nigeria, Nigeria

    Moringa oleifera has been found useful in treating water and a good chelating agent. On this account assessing its ability in treating heavy metal induced diseases and reduction of blood metal load might be positive. This was carried out using both seed and leave of M. oleifera in other to evaluate the part of the medicinal plant containing more activity. 1kg quantity of the powders of the leaves and seeds were exhaustively extracted with methanol and concentrated with rotator evaporator. Phytochemical analysis of the leaves and seeds extracts showed the presence of Alkaloids, flavonoids, saponins, resins, tannins, steroids, terpenoids, carbohydrate, proteins, oils, and reducing sugars. The result of the seed at 250mg/kg after induction of heavy metals gave 41.10ng/ml and 55ng/ml after treatment but gave 35.60ng/ml after induction and 26.63ng/ml after treatment at 500mg/kg dose. The leaves at 250mg/kg showed 35.73ng/ml after induction and 27.63ng/ml after treatment but 500mg/kg gave 36.36ng/ml and 27.10ng/ml after induction and treatment respectively. This gave the percentage different after administration of the seed extract at 250mg/kg, 500mg/kg as 6.2%, 25.77% and the same dose of leaves extract at as 22.67%, 25.47% respectively. Leaves of Moringa oleifera is more potent in reduction of heavy metal in the blood of white albino rats than the seed. KEY WORDS: Moringa oleifera , Heavy metals, Phythochemicals, Medicinal plants.

    Time:

    Title: Validation of ethnopharmacological uses of Piper sylvaticum (Roxb.) for its antimicrobial, anthelmintic and antidiarrheal properties

    Md. Nazim Uddin Chy
    International Islamic University Chittagong, Bangladesh.

    Biography
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    Biography

    Md. Nazim Uddin Chy
    International Islamic University Chittagong, Bangladesh.

    Md. Nazim Uddin Chy received his Bachelor’s degree in Pharmacy from International Islamic University Chittagong, Bangladesh in 2015. He is a Senior Researcher and co-founder of GUSTO A Research Group. His major field of interest is Phytotherapy research in the area of pain, neurobiology of pain, mental disorders including depression and anxiety and also neurodegenerative diseases such as Alzheimer’s disease, dementia, Parkinson’s disease and Huntington’s disease in animal models. He has a profound interest in learning new skills in the field of pharmacology, complementary and alternative medicine, natural medicine and pharmacotherapy as well.



    Abstract
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    Abstract

    Md. Nazim Uddin Chy
    International Islamic University Chittagong, Bangladesh.

    Piper sylvaticum (Roxb.) belongs to the family Piperaceae, commonly known as “Paan pata” in Bangladesh. It is widely used as traditional medicine for the management of asthma, chronic cough, cold, headache, diarrhea, wounds in lungs and tuberculosis. The present study is designed to scientifically verify the antimicrobial, anthelmintic and antidiarrheal activities of methanol extract of Piper sylvaticum leaves (MEPS). Fresh leaves of Piper sylvaticum were collected, dried in the shade, powdered and extracted with methanol. Then, the MEPS investigated for antimicrobial activity by disc diffusion technique and the anthelmintic activity was tested against aquarium worm (Tubifex tubifex) where the antidiarrheal activity evaluated by using a castor oil induced diarrhea model in mice. In the antimicrobial screening, the extract showed an average zone of inhibition 7-15 mm and highest effect noticed against Bacillus cereus with the zones of inhibition 15 mm at 1000µg/disc. For anthelmintic activity, the extract showed a moderate effect (paralyzed and death time 4.18 & 25.42 min respectively for MEPS at 10mg/ml) in comparison with standard drug levamisole. For castor oil-induced diarrhea, the MEPS at doses of 200 and 400 mg/kg produced a significant reduction in the frequency of diarrhea compare to loperamide (5mg/kg). Our current study has shown that the MEPS has antimicrobial and anthelmintic activities as well as possesses strong antidiarrheal actions. And the results justify its ethnomedicinal use through further bioactivity-guided isolation is necessary to obtain pharmacologically active secondary metabolites.

    Time:

    Title: Phytochemical and pharmacological study of volatile fractions from Eucalyptus camaldulensis leaves and their potent effects on isolated aortic and tracheal rings

    Dlzar Kheder
    University of Pavia, Italy.

    Biography
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    Biography

    Dlzar Kheder
    University of Pavia, Italy.

    Dlzar A. Kheder has expertise in teaching experience and research for more than 14 years as a staff of the universities, with general management skills in administration and leadership experience. His extensive background in Molecular Physiology, and he worked as a Postdoc in University of Pavia, Italy. He has experience in many techniques, including cell culturing (human endothelial progenitor cells, mouse brain endothelial cells, patients-derived colorectal cancer cells, and HeLa cells,hCMEC) and epifluorescence Ca2+ imaging during his postdoc in physiology department. Further expertise he has in heart perfusion system, Organ bath systems, during his research work as a staff of university of Zakho. Moreover he gain expertise in natural product extraction and Gaschromatography during his work as a researcher in organic chemistry in university of Pavia, in the field of natural products with pharmacological effects.



    Abstract
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    Abstract

    Dlzar Kheder
    University of Pavia, Italy.

    The current study represents the first complete report on constituents of different volatile fractions (VF) from Eucalyptus camaldulensis leaves, as well as their effects on isolated aortic and tracheal rings. Four different volatile fractions obtained by hydrodistillation (VFS, VFW, VFA and VFAr) showed different qualitative and quantitative compositions, though the major constituents in all the four samples were monoterpenes. The comparison of VFA and VFAr compositions with VFW clearly shows that, as expected, the aqueous phase collected in the condenser was more enriched in polar oxygenated monoterpenoids and sesquiterpenoids than in non-polar hydrocarbons. In conclusion, an important general consideration can be drawn about essential oil distillation from plants, namely, that significant amounts of oxygenated components can be dispersed in the aqueous phase collected in the condenser. Therefore, a more realistic analysis of plant volatile fraction must take into account not only the composition of the oily phase separated from the hydrosol, but also the mixture of polar compounds dispersed in water. The different VF induced vasorelaxation in concentration-dependent manner on isolated aortic rings. Both 1,8-cineol and VF produced a potent relaxation in rat tracheal rings precontracted with ACh, whereas the relaxant effect of VFs on tracheal rings pretreated with nifedipine was significantly inhibited. Pretreatment of tracheal rings with TEA and L-NAME did not alter dose-dependent relaxation. These results suggested that KCa channel and NO pathway were not involved in the relaxation on tracheal rings induced by VFs. Further, there was no relaxant effect induced by cumulative additions of VFs samples to tracheal rings preincubated with the Ca2+ channel blocker nifedipine, indicating as a conclusion to the participation of Ca2+ channels. Keywords: Eucalyptus camaldulensis, volatile fractions, GC/GC-MS, K+ channels, Ca2+ channels, tracheal ring relaxation

    Time:

    Title: The effect of ethanolic extraction of fresh Zingiber officinale against some microorganisms activities

    Hisham Osman
    AlJouf University, Saudi Arabia.

    Biography
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    Biography

    Hisham Osman
    AlJouf University, Saudi Arabia.



    Abstract
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    Abstract

    Hisham Osman
    AlJouf University, Saudi Arabia.

    All cultures and societies in Arabic countries has go strong knowledge about folk medicine, one example the using of Zingiber officinale (Ginger) to treat many diseases such as nausea, stomach ache, diarrhea and bleeding, inflammation and also used in sexual dysfunction. In this is study the extraction of fresh Ginger was done by ethanol 70%. The effect of extraction of Ginger was examined on many types of bacteria E. coli, Staphylococcus aureus, Bacillous and Pseudomonas, beside two types of fungal; Candida albican and Aspergillus niger. Moreover, antimicrobial susceptibility test was done by using cup plate assay method. The results showed that the inhibition zone of isolated bacteria as following: in Bacillus subtilus was found (14.5 mm), Pseudomonas aerigonosa was found (13 mm). Moreover, the study found the effect of Ginger on antifungal activity as following: in Candida albicans was found (13.5 mm) and Asperogillus niger was found (16.5 mm). Our data revealed that, the ethanolic extract of fresh Ginger has highly effectiveness against many types of bacterial activities and also against fungal activities. Key words: Medicinal plant, bacteria, fungal.

    Time:

    Title: Production Purification And Characterization Of Thrombolytic Protein Derived From Microorganisms

    Sharav Desai
    Pioneer Pharmacy Degree College, India.

    Biography
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    Biography

    Sharav Desai
    Pioneer Pharmacy Degree College, India.



    Abstract
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    Abstract

    Sharav Desai
    Pioneer Pharmacy Degree College, India.

    A non- toxic, direct acting enzyme with fibrin-specific activity produced and purified from newly isolated Streptococcus species. Fibrin plate method and In vitro blood coagulation assay were used to isolate the species producing thrombolytic protein. Identification and characterization of the strain were done using Bergey's Manual of Systemic Bacteriology and Advance Bacterial Identification System. Media and conditions for the production were designed and optimized. Time profile study was conducted to find optimum time for production. Three-stage purification system including salting out, dialysis and chromatography used to purify the protein. SDS-PAGE was performed to determine the homogeneity of the purified protein. Bands of concern in SDS –PAGE gel were excised and indigested with trypsin to perform Peptide Mass Fingerprinting. Agilent Infinity LC system coupled with an Agilent 6530 Accurate–Mass Quadrupole Time–of–Flight (Q–TOF) detector was used to identify and to study the protein. Fragment spectra were searched and compared to NCBI Non–redundant protein database using Agilent spectrum mill search engine. Multiple sequence alignment, Profile scan and statistical analysis of the sequence was conducted using various online proteomic tools available. Homology modeling of protein was carried out using Phyre2 and refined with the Galaxy WEB server. Five models were generated and evaluated by ERRAT, ANOLEA, QMEAN6, and Procheck. Key Words: Thrombolysis, Streptokinase, In-Silico, LCMS/MS-Q-Tof

    Time:

    Title: Nanosized Soy Phytosome-Based Thermogel Formulation for Treatment of Obesity, Characterization and In Vivo Evaluation

    Nermeen khalil
    Nahda University Beni-suef , Egypt.

    Biography
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    Biography

    Nermeen khalil
    Nahda University Beni-suef , Egypt.

    Nermeen Magdy is a demonstrator at pharmaceutics department Pharmacy College Nahda University Beni-suef Egypt that has an experience in formulation and evaluation of different dosage forms besides ability of teaching different courses. Foundation is based on phytosome as a nanotechnology advanced preparation that is perfect in interaction with natural active constituents to yield a high entrapment efficiency and high ability of transdermal drug delivery. Presenting author details Email: nermeen.magdy@nub.edu.eg Contact no:02-01200554113 Full name: Nermeen Magdy Abd El-Sater Khalil. Contact number: 02-01200554113 Twitter account: nermo christ@dr_nermochrist Linked In account: https://twitter.com/dr_nermochrist Facebook account: DR-Nermo magdy Linked in account: https://www.facebook.com/drnermomagdy



    Abstract
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    Abstract

    Nermeen khalil
    Nahda University Beni-suef , Egypt.

    Obesity has become an increasing problem over recent years. Nano lipo-vesicles hydrogels of soy saponin were formulated and evaluated in an attempt to reduce the size of adipose tissue cells through percutaneous absorption. Phytosome formulations were prepared with four different techniques: solvent evaporation, anti solvent precipitation, co-solvency and mechanical dispersion. Best formulae was selected by the means of the highest entrapment efficiency, minimum particle size and maximum drug release and then evaluated for successfull complex formation by means of FTIR. Particles zeta potential was detected and particles shape was evaluated using TEM to insure particles spherical shape. Selected phytosome formulae was involved into selected hydrogel formulae after evaluation of different plain hydrogel formulations for its calrity.homogenity ,PH ,gel transforming temperature and viscosity study Obtained phytosomal hydrogel formulae was then re-evaluated for its clarity, homogeneity, PH and gel transforming temperature and for its rheology behavior and permeation study. In vivo study was done to ensure anti -obesity effect of soy phytosomal hydrogel. Concisely, soy phytosomal hydrogel was found to have the ability to reduce the size of adipose tissue cells in male albino rats.

    Time:

    Title: Interleukin-28B plasma levels; a possible novel biomarker for Schistosoma mansoni/ hepatitis C virus co-infection prognosis

    Samuel Tanas Melek
    Delta University for science and technology, Egypt.

    Biography
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    Biography

    Samuel Tanas Melek
    Delta University for science and technology, Egypt.



    Abstract
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    Abstract

    Samuel Tanas Melek
    Delta University for science and technology, Egypt.

    Objectives: We aimed to studythe effect of Schsitosoma mansoni co-infection with hepatitis C virus (HCV) on IL-28B levels.Design:We collected plasma from107outpatients(range30–81 years old) from six governates of Delta, Egypt attending Kasr Al-Aini Hospital, Cairo, Egyptin 2012–2014. Subjects were divided to three groups, 35 healthy controls, 50naïve chronic HCV patientsand 22S. mansoni/HCVco-infected patients.For all participants,anti-schistosomal antibodies levels, hepatitis B surface antigens (HBsAg), HCV viral loadand routine liver function tests were measured. We assayed IL-28B and IFN-γ plasma levels for all participants.Results:We found that IL-28B levelsweresignificantly higher in S. mansoni/HCV co-infected patients than in HCV mono-infection. IFN-γ and IL-28B levels showed positive correlation in both infected groups. Patients with high HCV viral load had significantly higher IFN-γ and IL-28B levelswhether suffering from mono- or co-infection. Conclusions: A strong link between IFN-γ and IL-28B in naïve chronic HCV patients whether mono- or co-infected with S. mansoni. This suggeststhat co-infection with S. mansoni might not affect IFN-γ levels, however, significantly increases IL-28B levels.Therefore, IL-28B plasma levels might be a useful novel biomarker forprognosis and therapy ofS. mansoni/HCV co-infection. Keywords Hepatitis C Virus, Schistosoma mansoni, IFN-γ, IL-28B, chronic liver diseases

    Time:

    Title: Antioxidant and anti-inflammatory effects of lycopene on L-arginine induced acute pancreatitis

    Naglaa Khedr
    Tanta University, Egypt.

    Biography
    χ

    Biography

    Naglaa Khedr
    Tanta University, Egypt.



    Abstract
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    Abstract

    Naglaa Khedr
    Tanta University, Egypt.

    Background: Oxidative stress and subsequent activation of inflammatory mediators may play an important role in the development of acute pancreatitis (AP). Lycopene (LYC) a natural carotenoid has antioxidant scavenger capacity and inhibits inflammation in many experimental models. Objective: The study was designed to investigate whether lycopene can ameliorate L-arginine induced pancreatitis in rats and to elucidate the underlying mechanisms of these effects. Methods: Forty-eight adult male Wistar rats were divided into four equal groups: control group (rats received vehicle orally for 10 days), AP group (3 g/kg L-arginine, single i.p. injection on 10th day of experiment), LYC group (50 mg/kg lycopene, orally, once daily) and methylprednisolone (MP) group (30 mg/kg, orally, once daily) for 10 days prior to L-arginine injection. Then rats were sacrificed 24 h after L-arginine injection. Inflammation was assessed by measuring pancreatic tumor necrosis factor-alpha (TNF-α) concentration, myeloperoxidase activity (MPO) and gene expression of inducible nitric oxide synthase (iNOS). Oxidative stress was quantified by measuring pancreatic content of nitric oxide (NO) and reduced glutathione (GSH). Serum α-amylase and lipase activities were measured and histopathological studies of pancreas were done. Results: LYC group showed significant reduction in TNF-α and MPO activity and down-regulated iNOS gene expression. Pancreatic NO concentration and edema were reduced in LYC treated group. LYC also increased GSH content. Serum α-amylase and lipase activities were reduced by LYC treatment. The histological features of AP were all improved in LYC treated group. Conclusion: Lycopene treatment ameliorated inflammation and reduced oxidative stress in AP induced rat model. Keywords: Acute pancreatitis, iNOS, Lycopene, Myeloperoxidase, Nitric oxide, TNF-α

    Time:

    Title: Dopaminergic Agonists Improve Obesity by Virtue of its Action on Lipid Profiles and Leptin

    Ghada Al-Ashmawy
    Tanta University, Egypt.

    Biography
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    Biography

    Ghada Al-Ashmawy
    Tanta University, Egypt.



    Abstract
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    Abstract

    Ghada Al-Ashmawy
    Tanta University, Egypt.

    BACKGROUND: The efficacy of a non-prescription drug to support weight loss programs has yet to be compared. This clinical trial investigates the comparability of the lipase inhibitor orlistat and the dopaminergic agonist bromocriptine. METHODS: Seventy five obese females were randomized into three groups according to treatment received; obese control group (OC; n = 25), orlistat group (OR, n = 25, 120 mg capsules, three times a day) and bromocriptine group (OB, n= 25, 20 mg tablet, once a day). This prospective observational study was conducted with normocaloric diet for eight weeks. Serum concentration of leptin and lipid profile were measured, along with body Mass Index (BMI) at baseline and after the study. RESULTS: Bromocriptine treatment (OB) caused an increase in serum leptin concentration compared to OC and OR groups (ANOVA, p < 0.01). Beneficial changes in anthropometric and BMI values were observed following orlistat and bromocriptine administration with the greatest advantage seen in the OB group. CONCLUSIONS: Beneficial effects were observed on weight loss, and body composition in all examined groups, with the greatest advantage on serum leptin being associated with the bromocriptine treatment. We find these strategies more promising for the treatment of obesity and its related complications in obese women. KEYWORDS: Obesity, Bromocriptine, Orlistat, Weight loss, Leptin

    Time:

    Title: CYP3A5 genotyping by allele-specific PCR and melting curve analysis using three primers in single tube

    Geon Park
    Chosun University, South Korea.

    Biography
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    Biography

    Geon Park
    Chosun University, South Korea.



    Abstract
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    Abstract

    Geon Park
    Chosun University, South Korea.

    Introduction CYP3A5 is a liver enzyme that metabolized about 50% of medications, including tacrolimus. CYP3A5*3/*3 genotype is nonexpressors of CYP3A5 and metabolize some CYP3A substarates more slowly than CYP3A5 expressor genotypes (CYP3A5*1/*1 and *1/*3). To predict a metabolic ability of tacrolimus, we developed rapid CYP3A5 genotyping of CYP3A5 by allele-specific PCR and melting curve analysis using three primers in single tube. Methods We used one common forward primer and two allele-specific reverse primers that were G-specific primer with GC tail and A-specific primer without GC tail. The accuracy of CYP3A5 genotyping using the analysis was assessed in 100 DNA samples from normal persons, with Sanger sequencing as the reference method. Results Frequencies of CYP3A*1/*1, CYP3A5*1/*3, and CYP3A5*3/*3 by allele-specific melting curve analysis were 3, 44, and 53 in 100 samples, respectively. The concordance rate between the analysis and Sanger sequencing was 100%. Conclusion Genotyping of CYP3A5 by allele-specific PCR and melting curve analysis was fast and reliable for routine laboratory testing for prediction of tacrolimus metabolism.

    Time:

    Title: Small molecule discovery for the treatment of Alzheimer's disease by virtual screening and pharmacological evaluation

    Nerlis Pajaro Castro
    University of Sucre, USA.

    Biography
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    Biography

    Nerlis Pajaro Castro
    University of Sucre, USA.

    I am a pharmaceutical chemist, with a masters degree in pharmaceutical sciences and PhD candidate in environmental toxicology. At the present time I am associate professor at the faculty of health sciences of the University of Sucre. My work in research is focused on the area of famacology, toxicology, in silico drug design and chemistry medicine. Therefore, since several years I have been working on several research projects related to discovering molecules for the treatment of Alzheimer through in silico search and pharmacological evaluation, and evaluation of the toxicity of organic compounds using the insect Tribolium castaneum as in vivo model.



    Abstract
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    Abstract

    Nerlis Pajaro Castro
    University of Sucre, USA.

    Current treatments for Alzheimer's disease are focused on symptomatic threrapy, since not targets have been identified. The aim of this study was to discover small molecules acting on β-Secretase, γ-Secretase, Pin1 and Cdk5 proteins, and its pharmacological evaluation. The proteins 3D structures were downloaded from the PDB database and were docked, using AutoDock Vina, with molecules having drug-like properties. Initial analysis results reveal four potential compounds capable of binding to evaluated proteins which have affinity values ranging between -6.8 and -9.1 Kcal/mol. The interaction of ligands 84554447, 84577234, 84577855 and 84378305 with 4U84 protein (PDB ID), allowed the identification of residues SER32, ALA31 and LYS97 as the main amino acids involved in the protein-ligand docking. In the case of the 4UPC protein, the residues identified were CYS230, VAL51, ALA172, PHE287, ARG285, TYR173 and THR227. With 3VF3 protein, the amino acids were GLN73, THR218 and GLY217 and with 3O0G, the residues were ALA31, ASN144 and VAL64. Of the compounds having the highest affinity value, one was selected to perform the pharmacological evaluation. Thus, the compound 84378305 (Pubchem ID) was synthesized by chemical synthesis, to subsequently evaluate its neuroprotective effect, by the MTT and DHL release assays. The pharmacological evaluation showed that the synthesized compound has low cytotoxicity and is an effective neuroprotect. In conclusion, it was possible to find by in silico and experimental evaluation a ligand capable of acting against proteins related to Alzheimer's disease and which possess neuroprotective activity.

    Time:

    Title: In-Silico studies and anticancer evaluation of some newly synthesized indolin-2-one derivatives as potential VEGFR-2 inhibitors

    Nishtha
    Jamia Hamdard, India.

    Biography
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    Biography

    Nishtha
    Jamia Hamdard, India.

    Nishtha Shalmali is presently working on development of new tyrosine kinase inhibitor compounds as a PhD Scholar in the Department of Pharmaceutical Chemistry, School of Pharmaceutical Education & Research, Jamia Hamdard. She has recently qualified CSIR-SRF and secured 2nd Best Poster Award for her group’s research work on thiazole-5-carboxylate derivatives as selective MAGL inhibitors at ITS, Ghaziabad (India). She has emerged as an effective presenter during various conferences. Nishtha has attained hands on specific skills in the field of Green Chemistry, Scale up techniques and Bulk drugs during her Post Graduation & has achieved impressive hold on medicinal, pharmaceutical & analytical laboratory techniques during her doctoral research.



    Abstract
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    Abstract

    Nishtha
    Jamia Hamdard, India.

    Statement of Research: Tyrosine kinase inhibitors and their potential in clinical application are well documented by dramatic examples like, Gleevec, Iressa and Nexavar etc. Several tyrosine kinase inhibitors are undergoing human trials and several are in the pipeline of drug discovery. Quick selection of epidemiologically relevant, drugable tyrosine kinase targets coupled to efficient lead finding and optimization needs more intervention in the area of high throughput cancer genome based molecular therapeutics. All these concerted effort may pave the silver lining to tailor made personalized cancer therapeutics. Experimental & Theoretical Orientation: Keeping in view their importance, twenty new substituted indolin-2-one containing imine derivatives (2a-2t) were synthesized and docked with eight different tyrosine kinase enzymes (Aurora A Kinase PDB: 3FDN, Aurora B Kinase PDB: 2VRX, human Abl kinase PDB: 3CS9, human CDK6-VCYCLIN PDB: 2EUF, C-MET PDB: 4XMO, EGFR PDB: 1M17, Focal Adhesion Kinase PDB: 2JKK, human VEGFR-2 PDB: 3VHE). On the basis of docking results, VEGFR-2 target was selected out of all kinase targets for the in-vitro enzyme assay. Enzymatic inhibition assay was performed for all twenty compounds using VEGFR-2 enzyme inhibition assay kit and IC50 was obtained for all compounds. Simultaneously, all compounds were sent to NCI, USA for sixty-cell line based anticancer screening, out of which fifteen compounds were selected for one dose anticancer assay. Findings: Compounds 2a (NSC: D-795068/1) and 2g (NSC: D-795071/1) were found potent during one dose anticancer screening and fulfilled the specified threshold for growth inhibition criteria of NCI and further selected for full panel five dose assay at 10-fold dilutions of five different concentrations. Both compounds 2a and 2g displayed Mid GI50 values of 1.69 µM & 1.54 µM respectively against the cell lines of Leukemia, Non-Small Cell Lung Cancer, Colon Cancer, CNS Cancer, Melanoma, Ovarian Cancer, Renal Cancer, Prostate Cancer and Breast Cancer. Conclusion: The results were found even better than the standard used (Fluorouracil) by NCI. In silico studies and ADME prediction also supported the potential of these compounds as tyrosine kinase inhibitors. It is expected that Tyrosine Kinase inhibition by the said compounds may deliberate a substantial therapeutic benefit over existing treatments for cancer.

    Time:

    Title: Database of biologically active peptides and proteins

    Ma Gorzata Darewicz
    Warmia and Mazury University in Olsztyn, Poland.

    Biography
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    Biography

    Ma Gorzata Darewicz
    Warmia and Mazury University in Olsztyn, Poland.

    Prof. M. Darewicz received PhD in agricultural sciences in 1992. In 1997 she was visiting scientist in Danish Technological Institut, in 1998 at Wageningen University (Holland). From 2008 she has been full professor. She was visiting professor at Universities in Spain, Greece, France, Germany. Research Interests: physicochemical, functional, technological and structural properties of proteins, modifications combined with structure and function relationships; effect of technology on the molecular and functional properties; identification of proteins and peptides by use of HPLC, MS, and UV spectroscopy; in silico, ex vivo and in vitro study of proteins and peptides in aspect of diet-related diseases.



    Abstract
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    Abstract

    Ma Gorzata Darewicz
    Warmia and Mazury University in Olsztyn, Poland.

    Peptides derived from food proteins affect biological, functional, immunological and sensory properties of food products. Proteins apart from their function as the nutrients act as the precursors of nutraceuticals with variety of functions. BIOPEP database of protein and peptide sequences has been designed mainly for scientists working in the area of food and nutrition (http://www.uwm.edu.pl/biochemia). BIOPEP database consists of sequence databases: proteins, bioactive peptides, allergenic peptides with their epitopes and sensory peptides. The information concerning peptide or protein covers its sequence; data about activity or taste; references or in the case of allergenic protein database reference, sequence of experimental and theoretically predicted linear epitopes. Sequence analysis options include the construction of profiles of the potential biological activity, epitopes or sensory activity, calculation of the quantitative parameters A and B useful for evaluation and classification of proteins as precursors of bioactive or sensory peptides as well as immunogenic fragments. The options available include also the simulation and design of proteolysis as well as data mining. BIOPEP contains also the collection of links to other databases and programs. Proposed workflows for use of database of bioactive peptide sequences cover among others: use sequences of peptides as queries for database screening or identification of peptides from BIOPEP among products of protein hydrolysis. Selected examples of applications of the database, described by other authors will be presented. To date, apart from the data concerning different biological properties of peptides, BIOPEP may serve as a tool supporting the experimental and theoretical studies on food-derived biopeptides.

    Time:

    Title: Inhibition of Nuclear Factor- B Reduces Brain Inflammation in Rats

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Biography
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    Biography

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Prof. Kaplanski completed his Ph.D. in pharmacology in the Department of Pharmacology in the Hebrew University in Jerusalem (Israel). Thereafter, he completed his post-doctoral fellowship in the Department of Pharmacology in the Free University of Amsterdam (The Netherland). He mentored numerous M.Sc. and Ph.D. students in the fields of Pharmacology and exercise physiology. He has authored more than 100 peer-reviewed research papers.



    Abstract
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    Abstract

    Jacob Kaplanski
    Ben-Gurion University of the Negev, Israel

    Introduction. A large body of evidence suggests that inflammation is involved in the pathophysiology of several neurological disorders, including psychiatric illnesses, neurodegenerative diseases and stroke. Nuclear Factor- B (NF-B) is a cellular pathway that plays a prominent role in numerous immune and inflammatory responses in mammals. Aims. This study was undertaken to examine the effects of a selective NF-B inhibitor, JSH-23, on lipopolysaccharide (LPS)-induced inflammation in rats. Materials and Methods. Rats were treated with JSH-23 (10 mg/kg) through a single intraperitoneal (ip) injection. JSH-23 was administered at (i) 2.5 h before or at (ii) 1.5 h after LPS (1 mg/kg, ip) injection. At 4 h (i) and 2.5 h (ii) after JSH-23 administration, rats were sacrificed, blood was collected and different brain regions were excised. Levels of the inflammatory constituents interleukin (IL)-6, prostaglandin (PG) E2, tumor necrosis factor (TNF)- and nuclear phosphorylated p65 (P-p65) in plasma and brain were examined by specific ELISA kits. Results. The effects of JSH-23 on plasma and brain inflammatory mediators' levels differed between the pre- and - post-LPS administration schedule and between the various brain regions. Mostly, JSH-23 treatment reversed the changes in IL-6, PGE2, TNF- and P-p65 levels in plasma, frontal cortex, hippocampus and hypothalamus of LPS-treated rats. Conclusions. These results suggest that inhibition of NF-B may have a therapeutic potential for the treatment of inflammation-associated brain disorders.

    Time:

    Title: Community pharmacists’ roles in promoting asthma self-management: a cross-sectional study in Enugu state, South-East Nigeria

    Kosisochi Chinwendu Amorha
    University of Nigeria, Nigeria

    Biography
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    Biography

    Kosisochi Chinwendu Amorha
    University of Nigeria, Nigeria



    Abstract
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    Abstract

    Kosisochi Chinwendu Amorha
    University of Nigeria, Nigeria

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